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Offlineshroomeydan
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LC? RR, looking for you on this one...or anyone else of course!
    #7397578 - 09/11/07 05:59 PM (16 years, 4 months ago)

OK. I'm asking about the way RR does LC's with a grain jar and pure water. I was curious since you can get an extremely thick solution that can be diluted into apx. 100 syringes...I was curious to know 2 things.

1. Can I shake, extract, let recolonize and then store this grain jar in the fridge for months like I can with a honey lc to use as a master?

2. Instead of diluting, can I just use the initial highly concentrated solution to inject more grain like a standard LC inoculation, in hopes of getting more inoculation points than with a normal LC for optimal colonization speeds? (Logic thinking tells me I can, but with this hobby...I am never surprised that what you think will work is actually off by a hair and you end up with nothing.)


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OfflineDr_T
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Re: LC? RR, looking for you on this one...or anyone else of course! [Re: shroomeydan]
    #7398453 - 09/11/07 08:57 PM (16 years, 4 months ago)

I'm not RR, but I have done this.

1) Jars I've done this with come back strong. I spawn or case at that point, I don't save them- but they are perfectly fine jars. Be sure to suck up all the extra water, you don't want them to get funky.

2) I use that stuff full strength to inoc more jars and/or some real LC.
It's like g2g, only you don't have to open the jars.


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Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.


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OfflineRogerRabbitM
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Re: LC? RR, looking for you on this one...or anyone else of course! [Re: Dr_T]
    #7398613 - 09/11/07 09:32 PM (16 years, 4 months ago)

You can harvest liquid mycelium from the rye or wbs several times. After shaking the jar of grains to break them up, you can pour nearly a full pint of sterilized water into the jar, then shake again. Since there are nutrients in the grain juice/mycelium, you only need to pull a small amount of this solution into each syringe. Finish filling the syringe with sterilized water, and the mycelium will grow into it, expanding by several factors within a week.
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Invisibleshroomerite
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Registered: 06/09/06
Posts: 513
Re: LC? RR, looking for you on this one...or anyone else of course! [Re: RogerRabbit]
    #7399788 - 09/12/07 08:00 AM (16 years, 4 months ago)

That clears that up for me also. I am going to start do LCs this way as soon as I can get some Morel and portabelo cultures. I am going to make a huge outdoor morel patch for my grandfather. The soils is being preped now and in spring it will be inoculated.


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  "For best results, learn to work with nature rather than against it. Mycelium has an amazing ability to cope with less than optimal conditions, and will often fruit when a grower does everything wrong. However, do everything right and watch your performance go through the roof." RR



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