Home | Community | Message Board

MagicBag Grow Bags
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   Bridgetown Botanicals CBD Concentrates   PhytoExtractum Kratom Powder for Sale   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisible2012shaman
Stranger

Registered: 08/22/07
Posts: 338
Fuck its happening again!
    #7397563 - 09/11/07 05:56 PM (16 years, 4 months ago)

Dammit dammit dammit ,i tosed out 4 jars last week ,inoculated 4 more 2 days ago and i can already see cobweb in one of them and this time i made sure to be extremely sterile i even wore a garbage bag over my clean shirt im using 1 pints so i decided to do a little research on them and it turns out they are more prone to contams because they take longer to colonize so thats more bad news but this cant be why i see signs of contams on the second day ,is there something im forgeting or is it just bad luck?

BTW everyone says they take longer to colonize some say 4 weeks ,6 ,7 even 8 so on average how long should i expect to wait before birthing?


Extras: Filter Print Post Top
InvisiblePremedman1
Assistant to the insistent
 User Gallery


Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity
Trusted Cultivator
Re: Fuck its happening again! [Re: 2012shaman]
    #7397588 - 09/11/07 06:00 PM (16 years, 4 months ago)

Your syringe could be contaminated.

My grain jars always fully colonize in 1.5-3 weeks from multispore.
BRF takes a little longer due to it's dense consistency.

Are you pressure cooking your jars?


--------------------
Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.


Extras: Filter Print Post Top
Invisible2012shaman
Stranger

Registered: 08/22/07
Posts: 338
Re: Fuck its happening again! [Re: Premedman1]
    #7397639 - 09/11/07 06:12 PM (16 years, 4 months ago)

Could be but i got em from ralph and he seems to be pretty reliable and generous with replacements so im not going to go complaining about syringes when its probably my fault and no im not pressure cooking but i dont think thats it cause the last 4 jars were left to sit for a few days after i boiled them to make sure i did it properly anyway next time i innoculete some jars im gonna use quarts.


Extras: Filter Print Post Top
InvisiblePremedman1
Assistant to the insistent
 User Gallery


Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity
Trusted Cultivator
Re: Fuck its happening again! [Re: 2012shaman]
    #7397676 - 09/11/07 06:20 PM (16 years, 4 months ago)

No, you certainly don't want to use quart jars if you're using BRF as substrate. The jars you were using were already too big. Since your jars were twice as big, you should have at least doubled your boil time.

The PF tek was originally designed for use with 1/2 pint jars for a reason. It allows heat from the boiling water to completely penetrate the substrate. The bigger the jar, the lower the temperature your jars will reach.

Do yourself a favor and buy a PC, or stick religiously to a proven tek.


--------------------
Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.


Extras: Filter Print Post Top
Invisible2012shaman
Stranger

Registered: 08/22/07
Posts: 338
Re: Fuck its happening again! [Re: Premedman1]
    #7397726 - 09/11/07 06:31 PM (16 years, 4 months ago)

Wait i think i was confused when i said quarts cause what i meant was 1/4 pints.


Extras: Filter Print Post Top
OfflineDr_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 06/03/07
Posts: 1,839
Loc: Colorado
Last seen: 16 years, 4 months
Re: Fuck its happening again! [Re: 2012shaman]
    #7398440 - 09/11/07 08:54 PM (16 years, 4 months ago)

I think you are confused. How big a jar are you using, in cups? Like get a measuring cup, and pour water into an empty jar.


--------------------
Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.


Extras: Filter Print Post Top
Invisible2012shaman
Stranger

Registered: 08/22/07
Posts: 338
Re: Fuck its happening again! [Re: Dr_T]
    #7410959 - 09/14/07 09:03 PM (16 years, 4 months ago)

Why do you want it in cups? I know i am currently using 1 pints and instead of moving to half pints like i probably should i thought i would try quarter pints so they would colonize quicker seeing as my 1pints are going to take forever.Did i clear everything up or do we still have a miscommunication.


Extras: Filter Print Post Top
OfflineRastaimposta
Apothecary


Registered: 02/21/07
Posts: 227
Last seen: 4 years, 2 months
Re: Fuck its happening again! [Re: 2012shaman]
    #7410989 - 09/14/07 09:15 PM (16 years, 4 months ago)

You obviously havent told everything youve done. Did you use a glovebox/still air box? Where exactly did you inoculate (bathroom, kitchen, room, ect..) the jars? Did you flame/alcohol sterilize? Ralph has sterile syringes, so what happened must be on your end. I want to help you, as most of my shit fucked up early on because I was being a dumbass and using non-sterile techniques.


Extras: Filter Print Post Top
Invisible2012shaman
Stranger

Registered: 08/22/07
Posts: 338
Re: Fuck its happening again! [Re: Rastaimposta]
    #7411172 - 09/14/07 10:03 PM (16 years, 4 months ago)

The first time i was definetly a little sloppy but the second time i made sure i did it right ;i inoculated in my room made sure i had everything i needed ready i sprayed 50/50 bleach and water everywhere went to have a shower put on clean clothes went back to my room put a garbage bag over my shirt put two holes in the jar flamed the needle and injected the spores 4x made sure to breathe away from the jars and even sprayed a little during the whole process ,no glove box or air filter.The more i think about it the more it looks like a contaminated syringe ,out of three syringes(B+,PR,EQ) only the PRs havent been contaminated and everytime the B+ gets cobweb and red mold right down the side i injected the spore solution and the EQ gets green mold down the side as well.


Extras: Filter Print Post Top
Offlineheadieherbs
originalgangster (oflove)
 User Gallery


Registered: 09/27/06
Posts: 523
Loc: around the bend
Last seen: 16 years, 1 month
Re: Fuck its happening again! [Re: 2012shaman]
    #7411502 - 09/14/07 11:28 PM (16 years, 4 months ago)

dude its probably your syringes how old are they and where did you get them?

and it sounds like you poked holes in your lids after you PC'd and right before you inoculated?? thats not right.

and it should take like 2 weeks for your jars to colonize, any longer and somethings wrong


--------------------
please don't take away my highway shoes..

Write In Your Vote.com - US Politics. For the People, by the People.


Everything posted by the user(s) Headieherbs is either an outright lie or a work of complete fiction


Extras: Filter Print Post Top
Offlinexhooliganx
Munky
Male User Gallery

Registered: 03/09/06
Posts: 900
Loc: reno, nevada
Last seen: 2 years, 8 months
Re: Fuck its happening again! [Re: headieherbs]
    #7411537 - 09/14/07 11:42 PM (16 years, 4 months ago)

well its probably because you're inoculating in open air. with all that you're doing to be clean the air is still filthy as fuck. even an old syring shouldn't have contams in them if they where made sterile. i'd say that your boiling times is another big factor.


--------------------


Extras: Filter Print Post Top
OfflineThe shroomy 1
Luminous beings surround me
Male User Gallery


Folding@home Statistics
Registered: 03/27/07
Posts: 5,543
Loc: The Aether
Last seen: 5 months, 5 days
Trusted Cultivator
Re: Fuck its happening again! [Re: 2012shaman]
    #7411903 - 09/15/07 02:55 AM (16 years, 4 months ago)

I know this may be a far stretch, but where do you store you garbage bags? I store my inside trash bags next to the trash can in the pantry, (obviously a very contaminated area!), and I store my outside "yard" bags in the garage, (an area that has probably been exposed to every mold spore known to man!). You see where I'm going with this? I have personally never gotten a contaminated syringe from Ralphsters. That's not to say that it can't happen, but it's unlikely. I suggest rethinking your process and watch this video.http://video.google.ca/videoplay?docid=3286686859329905674


--------------------


AMU Q&A thread.


Extras: Filter Print Post Top
Invisible2012shaman
Stranger

Registered: 08/22/07
Posts: 338
Re: Fuck its happening again! [Re: The shroomy 1]
    #7412495 - 09/15/07 09:11 AM (16 years, 4 months ago)

My garbage bags are in my pantry and my garbage is in a cuboard under the sink ,but anyway i didnt use the same place for both innoculations ,first time i was at my friends house second time at my house and both times the exact same thing happened PRs come out fine ,B+ gets cobweb and red mold ,EQ gets green mold.Even if it a syringe i will still always order from ralph hes got the best selection and can ship to me faster than anyone else.


Extras: Filter Print Post Top
OfflineanarchOi
Ellenalien is fat.
Male User Gallery


Registered: 08/06/07
Posts: 2,293
Loc: ASE Flag
Last seen: 12 years, 11 months
Re: Fuck its happening again! [Re: 2012shaman]
    #7412526 - 09/15/07 09:23 AM (16 years, 4 months ago)

it sounds like the contam prolly came from either your jars, or your needle to me

wash out your jars really well with a 1-10 bleach/water solution
remember to flame your needle well! don't melt the plastic thingy :-P


i don't see anything wrong with using a pint jar
i've ONLY used pint jars for my cakes, and never had a contam
i highly doubt the size of your jar is the reason for your contams
are you remembering to cover with foil?
you should try for a 100% sterile technique
use 100% while innoc'n, then transport them only in their sterile incubator (the one you shoulda sprayed your watered down bleach solution in<very fine mist> and let dry) to the pre-sprayed closet or something, spray the closet regularly, check your jars only after spraying well

bleach is your friend (as long as you keep your mycelia safe from it), PERFECT your sterile technique, over look nothing and keep on trying

i have an EQ cake growing in a pint jar if you guys seriously think the size of the jar matters


--------------------


Extras: Filter Print Post Top
Invisible2012shaman
Stranger

Registered: 08/22/07
Posts: 338
Re: Fuck its happening again! [Re: anarchOi]
    #7412550 - 09/15/07 09:33 AM (16 years, 4 months ago)

Wow so youve only ever used pints i guess you can tell me how long on average im gonna half to wait before i can birth these cakes ,i can be kinda impatient sometimes so im gonna try and knock up a couple of 1/4pints i figure they should only take about two weeks to colonize


Extras: Filter Print Post Top
OfflineanarchOi
Ellenalien is fat.
Male User Gallery


Registered: 08/06/07
Posts: 2,293
Loc: ASE Flag
Last seen: 12 years, 11 months
Re: Fuck its happening again! [Re: 2012shaman]
    #7412618 - 09/15/07 10:05 AM (16 years, 4 months ago)

with multispore it all depends on how many precious CC's you shoot up your cake with

my personal fav way to do it, is to use 2 innoc points and squirt 1 cc to the left and right of each point, using about a third of the syringe
if done that way, i've had them colonize in under 3 weeks

if done with less, which i've also done, i've seen them not grow for almost 2-3 weeks, then start and colonize in under 3 (odd but happened with one batch, i don't think it was the jar, probably the syringe)

but with less i've also seen them colonize in under 3 weeks

less being like 2 cc's total in the jar


one thing i will say against the pint jars is that they seem to stall when they get to the bottom pretty consistently
this is easily solved by flipping the jar and mocking birth inside the jar

the mycelia usually takes that little bit of FAE and finishes colonizing in a matter of days

though i don't advise it for risk of contams
i usually flip mine when they are about 75% done and the other 25% is colonized in like 3-4 days with mycelia bursting through the center of the uncolonized area and helping finish the job


i like the pint jars mostly because i break the cakes up and case them so i can take prints and such and it provides alot of substrate

but also the only cakes i've ever fruited were pint jars like i said
and after about 3-4 flushes of remarkably healthy mushrooms the cake had shrank so much that it would easily fit in a half pint jar and it still fruited weakly before being retired

i just think that the bigger the cake, the longer it'll last (i've fruited a single cake for almost 2 months, maybe more)


--------------------


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   Bridgetown Botanicals CBD Concentrates   PhytoExtractum Kratom Powder for Sale   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* Re: Are there some common signs of contamination a beginner should know? Anonymous 1,954 3 12/15/99 07:38 PM
by JoHnNySc
* Contaminated syringes? Too old?? Visigoth 516 3 08/06/02 02:30 PM
by mickey_rourke
* When Contamination Strikes! phrozendata 699 5 07/11/02 04:50 AM
by Frog31337
* Major "?" about differences: multispore vs. clonin mrdasani 2,991 13 04/27/02 06:59 PM
by delysid_1
* Contamination? Scarface 1,028 3 12/19/01 06:38 PM
by flaw
* rancid CrabbyAss 3,389 17 06/27/02 11:58 PM
by bluefaced
* Nothing Has Happened! sillvyr 1,887 18 05/31/18 09:01 AM
by Tormato
* Best way to get rid of contaminated jars? Chemical_Smile 1,741 7 04/14/02 02:06 AM
by Roadkill

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,019 topic views. 21 members, 218 guests and 62 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.007 seconds on 14 queries.