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gmuralid
Holy Cow


Registered: 08/05/07
Posts: 405
Loc: India
Last seen: 7 years, 3 months
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Honey LC Syringe growth parameters?
#7395015 - 09/11/07 03:29 AM (16 years, 4 months ago) |
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Ok, so Ive been reading a bit about the honey LC teks and such, but here is a question I couldnt find the answer to.
I had some used spore syringes (empty with a few spore clumps still hanging on) and so I microwaved some honey/water for 2/3 minutes (well past boiling point with my microwave), then simmered for a few minutes , let sit for about an hour till it was cool.
I then sucked up the honey water (70 ml) and divided the amount into the syringes (5 empties), so 10ml per syringe (which is the capacity of each syringe). Remaining 20 ml was used with a carboard tek in a petridish.
This was yesterday. I put the syringes with a colonizing ziplock bag I have inside a carboard cover in my extra room by the window (gets good temps).
Today, less than 24 hours later, I checked the syringes (was so bored I had to look) and found 2-3 mm clouds attached to the spore clumps in the syringes. I dont have pics right now (Could probably upload in 2-3 days), but from the pictures I have seen of the honey LC teks here, it looks like white clouds of mycelium after a few days of growth.
Is this normal? Can someone help me with growth parameters for mycelial growth in a honey/water syringe?
I dont know if this is super fast mycelium, super contam or super honey sediment clouds. Thing is, they are attached to the spore clumps, leading me to believe mycelial growth, but I cant be sure without help from you guys.
Help!!!
-------------------- Wilderness. It defines me.
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myCo_psyCo
a mycologist inthe making



Registered: 06/11/05
Posts: 969
Loc: own private hell
Last seen: 10 years, 8 months
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Re: Honey LC Syringe growth parameters? [Re: gmuralid]
#7395120 - 09/11/07 04:40 AM (16 years, 4 months ago) |
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IME i have had emptys and filled them back up with water and had enough to innoc 5-6 jars with no prob. your plan sounds fine but you dont want to suck boiling hot LC into your spores or it will kill them, i hope you let your solution cool first
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gmuralid
Holy Cow


Registered: 08/05/07
Posts: 405
Loc: India
Last seen: 7 years, 3 months
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Re: Honey LC Syringe growth parameters? [Re: gmuralid]
#7395229 - 09/11/07 06:07 AM (16 years, 4 months ago) |
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Quote:
gmuralid said:
..... then simmered for a few minutes , let sit for about an hour till it was cool.
I then sucked up the honey water...blah blah
Yup!
-------------------- Wilderness. It defines me.
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gmuralid
Holy Cow


Registered: 08/05/07
Posts: 405
Loc: India
Last seen: 7 years, 3 months
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Re: Honey LC Syringe growth parameters? [Re: gmuralid]
#7395244 - 09/11/07 06:17 AM (16 years, 4 months ago) |
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Question is though, if something got in while I was sucking up honey juice, then what will it look like? I mean, I dont know if this mycelium should be so strong in a day, no?
-------------------- Wilderness. It defines me.
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StriplessRED

Registered: 09/05/07
Posts: 562
Loc: Helena
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Re: Honey LC Syringe growth parameters? [Re: gmuralid]
#7395356 - 09/11/07 07:21 AM (16 years, 4 months ago) |
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Can you post pictures?
Sounds like myc to me, but could very well be a contam
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Vegan
using the searchbutton



Registered: 08/16/07
Posts: 352
Last seen: 14 years, 6 months
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Re: Honey LC Syringe growth parameters? [Re: StriplessRED]
#7395429 - 09/11/07 07:53 AM (16 years, 4 months ago) |
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I had a lc that turned to myc super fast ..I was like OMG thats quick!!! turned outto be contaminated lc..good luckand try it ona control jar
-------------------- I came, I saw , I came back
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gmuralid
Holy Cow


Registered: 08/05/07
Posts: 405
Loc: India
Last seen: 7 years, 3 months
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Re: Honey LC Syringe growth parameters? [Re: Vegan]
#7395596 - 09/11/07 09:29 AM (16 years, 4 months ago) |
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Damn! Doesnt sound like mycelial goodness then huh? 
Ok anyway, Ill post some pics in 1-2 days, I have to get a new cable, since one of my friends took mine and now no one knows where it is.
Ill post them in a daily sequence then, coz I already took pics, and Ill get tomm. and day after on there as well, for comparison.
-------------------- Wilderness. It defines me.
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