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InvisibleWWorker
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Registered: 09/09/07
Posts: 395
making filling syringes from prints....
    #7393339 - 09/10/07 07:20 PM (16 years, 4 months ago)

Out of the methods described in the "mushroom info" area of this site, what method do y'all like best for filling syringes from prints?

Thanks,


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Edited by mbmcewen (09/15/07 09:56 AM)


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Invisibleorchidfanatic
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Registered: 08/12/07
Posts: 832
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Re: making filling syringes from prints.... [Re: WWorker]
    #7393386 - 09/10/07 07:28 PM (16 years, 4 months ago)

I dont know of the info area you speak of but I would follow RR's
video .. using PC shot glass wrapped in foil , PC 1/2 pint jar of distilled water.. , PC syringe wrapped in foil. umwrap the sterile syringe .flame the needle. fill it with sterile water from jar. squirt water into shot glass. scrape spores into shot glass. flame the needle. draw the spore/water from shot glass into syringe and cap
syringe.. done..


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InvisibleWWorker
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Registered: 09/09/07
Posts: 395
Re: making filling syringes from prints.... *DELETED* [Re: orchidfanatic]
    #7393445 - 09/10/07 07:41 PM (16 years, 4 months ago)

Post deleted by mbmcewen

Reason for deletion: The post is redundant



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InvisibleWWorker
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Registered: 09/09/07
Posts: 395
Re: making filling syringes from prints.... [Re: WWorker]
    #7393467 - 09/10/07 07:45 PM (16 years, 4 months ago)

I just bought the DVDs. Thanks for the info.


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Invisibleorchidfanatic
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Re: making filling syringes from prints.... [Re: WWorker]
    #7393470 - 09/10/07 07:46 PM (16 years, 4 months ago)

yeah the videos are nice... some claim everything is posted on you tube .. but I liked the videos... only thing they dont cover in the videos is fruiting methods except for straw logs and straw laundry basket ..but i liked the vids.. I liked the isolation and agar parts. . good luck


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Invisibleorchidfanatic
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Re: making filling syringes from prints.... [Re: orchidfanatic]
    #7393477 - 09/10/07 07:47 PM (16 years, 4 months ago)

oh I think a new video is coming out some time next year .. should be good .. i will get it when it comes out cool stuff like growing edible mushrooms on phone books ... wild !


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Offlinecbezzy01
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Registered: 09/06/07
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Re: making filling syringes from prints.... [Re: orchidfanatic]
    #7393756 - 09/10/07 08:46 PM (16 years, 4 months ago)

Here's the method I use

First completely take the syringe apart and boil them for about 20-30 minutes, then reassemble and suck up water and fill the syringe.
I then take my print, that is on foil and inside a little plastic baggie, I then squirt the water from the syringe directly into the baggie and scrape the spores into the water, I then suck up the solution and end up with very very dark syringes.

Best of Luck to You! :smile:


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Offlinehighc
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Re: making filling syringes from prints.... [Re: cbezzy01]
    #7394054 - 09/10/07 09:40 PM (16 years, 4 months ago)

i took a freshly open cap placed it in the bottom of a sterilized jar tightly closed it and let it sit. 24 hours later i took the cap out ate it and the spore print was at the bottom of the jar then i put about a half inch distilled water in the jar shook like crazy then pulled it into a sterilized syring. my syringe is almost black. but i havent used it yet so ic ouldnt tell you if it works but should be ok.


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OfflinePhreakr
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Registered: 07/23/07
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Last seen: 5 months, 8 days
Re: making filling syringes from prints.... [Re: highc]
    #7394239 - 09/10/07 10:28 PM (16 years, 4 months ago)

I used the Shot-Glass method aswell, but i dont have a Pressure Cooker, so I boiled water with the shotglass in it floating around for about 20 minutes, got my (already sterile because i got it from a friend who works in hospitals hehe) syringe, sucked up 10ml's of the boiled water. Let things cool then scraped some of the print (about 1/5th) into the shotglass (using the syringe tip) and squirted about 3 to 4ml's of the water out of the syringe into the shotglass, then sucked it back up. result = nice dark and viable syringe which i've already successfully used. going onto LC's now though. Oh, and i also flame and alcohol sterilised the syringe, and alcohol sterilised the shotglass right before use. best of luck! :mushroom2:


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