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OfflineNalim
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A new ergot thread: A few pics both macro and micro
    #7391770 - 09/10/07 12:29 PM (16 years, 4 months ago)

So I thought we could try this again.
I have shot some pictures of ergot both macro and some micro








There was a thread about cultivation of ergot earlier but it got locked because of the constant sidetracking.
Don't let that happen again people!

There is some good information in there though for those interested in cultivating this species:
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6607718/page/0/fpart/1/vc/1

Well I have some sclerotia and I was wondering if I could get suggestions about a procedure to revive the mycelium.
I have done a search and I've read the posts I could find on the subject(none about this species in particular but I figure it is pretty much the same for most fruit body producing fungi).
I'd love it if I could get some good links or if one of you experienced mycologists could give me your 2c about how I could succeed in this.


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Offlineabsinthoman
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Re: A new ergot thread: A few pics both macro and micro [Re: Nalim]
    #7392850 - 09/10/07 05:24 PM (16 years, 4 months ago)

Personnally,I would first put these sclerotia in distilled sterile water and blend everything in a sterile blender. Then I would try to inoculate a malt agar petri dish and see what happens. For instance, this petri dish could be made out of a special media who can reinforce the preselection of the producing strains. High sucrose, glycine and NaCl concentrations have been reported to favor the growth of colonies containing cells of the sclerotia type capable of synthesising alkaloids. That could be a first preselection.

Then I would isolate the producers by doing the Keller test on small part of some colonies and isolate these one if the result to the Keller test is positive. Since difference have been reported between producer and non-producer I would take note on the morphology of the producer I just isolated after the Keller test for my next isolation.

Then after that, you could play with liquid culture and stuff...


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OfflineWorkmanV
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Re: A new ergot thread: A few pics both macro and micro [Re: Nalim]
    #7393241 - 09/10/07 06:57 PM (16 years, 4 months ago)

Ergot sclerotia needs a cold wet treatment to germinate and form carpophores. Sclerotia is placed in moist sand in the refrigerator for at least a month (2 is better) and then brought to room temperature for around 5 weeks. The cold treated sclerotia can fruit directly in the moist sand or can be moved to moist filter paper in petri dishes. Good luck!


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OfflineNalim
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Re: A new ergot thread: A few pics both macro and micro [Re: Workman]
    #7393309 - 09/10/07 07:12 PM (16 years, 4 months ago)

Thanks I'll try that. I hope it's not too old, but I imagine it can take rather long resting periods.


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OfflineeVenom
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Re: A new ergot thread: A few pics both macro and micro [Re: Nalim]
    #7393648 - 09/10/07 08:28 PM (16 years, 4 months ago)

Mycelium is Mycelium don't matter if its from a fruit or sclerotia

you should read this Agar with died tissue

and mostly what RR has to say on that tread!

good luck!!!


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OfflineRogerRabbitM
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Re: A new ergot thread: A few pics both macro and micro [Re: eVenom]
    #7395300 - 09/11/07 06:46 AM (16 years, 4 months ago)

Quote:

Mycelium is Mycelium don't matter if its from a fruit or sclerotia





Not exactly. In addition, ergot is different from other sclerotia producing species such as mexicana or morel. I'd try what WM suggested. When I placed ergot sclerotia directly on agar, nothing happened.
RR


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OfflineNalim
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Re: A new ergot thread: A few pics both macro and micro [Re: RogerRabbit]
    #7395857 - 09/11/07 11:12 AM (16 years, 4 months ago)

Yeah, workmans advice seemed like a easy way to do it and it seems probable that it'll work..
I'll try that.


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Rodney Brooks on Robots
Nalim said: "Quoting yourself is retarded."


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Offlineabsinthoman
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Re: A new ergot thread: A few pics both macro and micro [Re: Nalim]
    #7396773 - 09/11/07 02:57 PM (16 years, 4 months ago)

Can we inoculate the agar plate from a biopsy of the fruitbody obtained from the sclerotia???


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OfflineNalim
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Re: A new ergot thread: A few pics both macro and micro [Re: absinthoman]
    #7396799 - 09/11/07 03:04 PM (16 years, 4 months ago)

That was my first thought when I started this thread but it seems like much more work than what Workman suggested.
I would bet that Ergot scletoria isn't naturally inclined to go back into a mycelium producing state unless it gets a coldchock first.


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Rodney Brooks on Robots
Nalim said: "Quoting yourself is retarded."


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Offlinecosmoline
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Re: A new ergot thread: A few pics both macro and micro [Re: Nalim]
    #7697307 - 11/30/07 12:07 AM (16 years, 2 months ago)

Any luck?


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OfflineNalim
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Re: A new ergot thread: A few pics both macro and micro [Re: cosmoline]
    #7698297 - 11/30/07 10:35 AM (16 years, 2 months ago)

Nope not yet.


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Rodney Brooks on Robots
Nalim said: "Quoting yourself is retarded."


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Offlinecosmoline
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Re: A new ergot thread: A few pics both macro and micro [Re: Nalim]
    #7698585 - 11/30/07 11:52 AM (16 years, 2 months ago)

What are you doing with it at this point?


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OfflineNalim
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Re: A new ergot thread: A few pics both macro and micro [Re: cosmoline]
    #7698648 - 11/30/07 12:05 PM (16 years, 2 months ago)

Followed WMs advice, put it on wet sand a week back, it's in the fridge.


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Nalim said: "Quoting yourself is retarded."


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Invisibleem_bre_O
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Re: A new ergot thread: A few pics both macro and micro [Re: Nalim]
    #7763726 - 12/15/07 09:58 PM (16 years, 1 month ago)

what ever come of this?


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InvisibleCheezit
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Re: A new ergot thread: A few pics both macro and micro [Re: em_bre_O]
    #7764603 - 12/16/07 02:05 AM (16 years, 1 month ago)

I used to see bags of rye with probably a couple quarts of ergot per 50 pounds. Now I only get market quality clean rye. Would be neat to check out.


Embryo It's probably still in the fridge.

Workman I like the idea of stratifying the sclerotia.


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Invisibledysphoria
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Re: A new ergot thread: A few pics both macro and micro [Re: Cheezit]
    #7776296 - 12/19/07 01:37 AM (16 years, 1 month ago)

i attempted this some months back nalim, to no avail.
hopefully your experiment will fare better than mine.

was the same principle followed as closely as possible.


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InvisibleBUDDHA_702
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Re: A new ergot thread: A few pics both macro and micro [Re: dysphoria]
    #7780291 - 12/20/07 02:18 AM (16 years, 1 month ago)

Why Cultivate ergot?
To make LSD?


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Offlinecanid
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Re: A new ergot thread: A few pics both macro and micro [Re: BUDDHA_702]
    #7780312 - 12/20/07 02:38 AM (16 years, 1 month ago)

NO. nobody is thinking of doing that, at all.


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InvisibleBUDDHA_702
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Re: A new ergot thread: A few pics both macro and micro [Re: canid]
    #7780329 - 12/20/07 02:56 AM (16 years, 1 month ago)

mod edit: off topic


Edited by Nalim (12/20/07 05:38 AM)


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OfflineNalim
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Re: A new ergot thread: A few pics both macro and micro [Re: BUDDHA_702]
    #7780489 - 12/20/07 05:39 AM (16 years, 1 month ago)

That is off topic. Don't turn this into a LSD discussion.
No further posts on that topic in this thread.


--------------------

Rodney Brooks on Robots
Nalim said: "Quoting yourself is retarded."


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