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Offlineanthony034
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simple LC worth it.
    #7389043 - 09/09/07 05:48 PM (16 years, 4 months ago)

i came across the simple LC teks on here, and i was just wondering if it was worth my time to try and start an LC with nothing but colonized rye berries, quart jarts, surgical tape, glass shards, and distilled water.

i was going to get casing down to a science before attempting an LC. but i have an over-abundance of grain to work with so....


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YES WE CAN




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OfflineXeluc
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Re: simple LC worth it. [Re: anthony034]
    #7389051 - 09/09/07 05:50 PM (16 years, 4 months ago)

YES! My very first grow i made an lc out of first. its a small time investment but it makes everything easier and faster in the end. btw, either boil grains in 1/2 pints (personally never have had a jar contam) or step up to a pressure cooker for the quart jars you said you have. don't boil those. waste of time.


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Offlineanthony034
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Re: simple LC worth it. [Re: Xeluc]
    #7389060 - 09/09/07 05:53 PM (16 years, 4 months ago)

Quote:

Xeluc said:
YES! My very first grow i made an lc out of first. its a small time investment but it makes everything easier and faster in the end. btw, either boil grains in 1/2 pints (personally never have had a jar contam) or step up to a pressure cooker for the quart jars you said you have. don't boil those. waste of time.




i have a PC, and i have a 100% success ratio on all my cakes as of late.(why i decided to move to casings.)

i know how beneficial LC is. i just wanna know if its worth it to try the easy tek using only distilled sterile water for a substrate, or just wait until i can work with agar, or at least dextrose?


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YES WE CAN




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OfflineXeluc
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Re: simple LC worth it. [Re: anthony034]
    #7389066 - 09/09/07 05:55 PM (16 years, 4 months ago)

oh...
no man, a lc in straight tap water wont do anything. go to your store and get karo or honey. 2-3 dollars. 4% by volume to water, whichever you use. oh, and i never bothered with cakes.i say its great your going to casings


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Offlineanthony034
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Re: simple LC worth it. [Re: Xeluc]
    #7389466 - 09/09/07 07:40 PM (16 years, 4 months ago)

Quote:

Xeluc said:
oh...
no man, a lc in straight tap water wont do anything. go to your store and get karo or honey. 2-3 dollars. 4% by volume to water, whichever you use. oh, and i never bothered with cakes.i say its great your going to casings




is this tek a joke then?
http://www.shroomery.org/65/Uncle-Lazlos-EZ-Mycelium-Syringe-Tek


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YES WE CAN




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Offlinenai
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Registered: 08/29/07
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Re: simple LC worth it. [Re: anthony034]
    #7389614 - 09/09/07 08:28 PM (16 years, 4 months ago)

yeah your going to have to place like the other guy said some kind of nutes to the water it doesnt have to been karo alot of people have used other things too


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Offlinechucklehead
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Re: simple LC worth it. [Re: anthony034]
    #7389666 - 09/09/07 08:45 PM (16 years, 4 months ago)

That's a pretty interesting TEK.  I'd call it a little on the risky side for a couple reasons. 

First it sounds a little bit contamination prone though I've read several people report that this method works well for them.  It's basically a form of jar to jar technique.  The difference here being it doesn't sound like you are supposed to try and keep this stuff around for very long.  Especially since you aren't providing them any more nutrition than what was in the sliver of cake from which they came.  It would definitely be worth trying IF the cake came from a know reproductive clone.  That brings me to point number two.

If you are taking mycelium from a jar made from a multispore syringe you may be growing some lazy mycelium.  Not every spore leads to sexual hyphae.  Sometimes you just have an asexual biological eating machine.  Sort of like some women after you marry them.  Everything looks great to start with then after a while you realize nothings going to happen.  Now what you thought was going to be a productive relationship has turned out to be a parasitic one where in you feed, house, and care for the other organism and get nothing in return other than the opportunity to watch them grow old, ugly, and hopefully die soon.  Where in the latter case you can collect the life insurance :smile:  In the former case you wasted time energy and substrate.  To back petal for a second if you are working from a "known to fruit" clone in the BRF cake I'd say you've got a good chance of making some sweet syringes on the fly this way if you don't contaminate the jar in some way.

Since you are having such great success with your cakes, why not clone some of your best fruits?  You can do this without agar. **see below**  Many folks have reported decent cloning success by simply taking samples from fresh specimens and putting them in a traditional nutrient rich liquid culture like the above examples of sterilized 4% honey or Karo solution.

**Liquid cultures are a risk just about any time and any way you do them.  You never know what's in the jar until you grow it out on a medium/substrate that lets the organisms in the solution mature to a recognizable point.  Agar being one of the most convenient since you can just throw away the petri if it's bad and it doesn't cost much.  You can always make test jars of WBS and see how they turn out.  That'll tell you if your syringe is any good.  I like agar because you use less solution to test if your syringe is crap or not and because if you have contaminated test medium then it's cheaper to throw out the plastic petri than a jar.  Also it seems to take less time to grow out on agar than in a BRF jar.  That's just in my experience others may think differently.  Bacteria shows up on the plate in no time and other molds like trich usually are recognizable within six days or less. 

Keep in mind I've only been doing this for about eight months now so take what I've said with that in mind.  So there may be flaws in my thought process above but they come from my perception of the series of LCs I've done so far.

Finally no I don't think the above link is a joke.  Though this part I don't know about.
Quote:

I place the lid loosely on the jar (rubber seal in place) and heat it at 400F for an hour.


  Overall it is an interesting idea for a fast syringe creation.  A very noteworthy idea.  That said I don't think I'd use it.  I see my future LCs moving away from the multispore variety and moving toward being clones of my fastest fruiters.


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Offlineanthony034
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Re: simple LC worth it. [Re: chucklehead]
    #7389768 - 09/09/07 09:09 PM (16 years, 4 months ago)

wow, thank you for elaborating way more than i initially expected anyone to.

with that said i will test this method, just because it's very very simple.


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OfflineeVenom
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Re: simple LC worth it. [Re: anthony034]
    #7390149 - 09/09/07 10:54 PM (16 years, 4 months ago)

well i love this tek you might like it to

here :Clone LC


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eVenom

"Some of us like a nice buzz, while others want to climb a screaming volcano naked to meet god."
RR


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Invisibleblood4blood
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Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: simple LC worth it. [Re: eVenom]
    #7390181 - 09/09/07 11:03 PM (16 years, 4 months ago)

this is all you need for doing an lc. im making one right now but skipping the step of using a syringe and just dropping spores directlly into the liquid ( this is done in a glove box though)




http://www.shroomery.org/forums/showflat.php/Cat/0/Number/7320513/an/0/page/0


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OfflineeVenom
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Re: simple LC worth it. [Re: blood4blood]
    #7390944 - 09/10/07 07:23 AM (16 years, 4 months ago)

Yes! you can do that is really easy and works like a charm!!

and yes it should be done in a glove box but i had great success in a small room with very still air and some air sanitizer!


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eVenom

"Some of us like a nice buzz, while others want to climb a screaming volcano naked to meet god."
RR


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Invisiblecloudtripper
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Posts: 175
Re: simple LC worth it. [Re: eVenom]
    #7391055 - 09/10/07 08:27 AM (16 years, 4 months ago)

I use Ottos LC tek here,

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5238137/page/0/fpart/1/vc/1

Very easy no PC needed and I have never had a contam from it yet, even after using the same LC over and over.


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Always come back again. Never come back the same.


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OfflineDigital Reality
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Re: simple LC worth it. [Re: cloudtripper]
    #7391276 - 09/10/07 09:54 AM (16 years, 4 months ago)

Why not just use apple juice or some kinda real bottled fruit juice. You don't need to PC it or anything. Just take a bottle of it sand a a spot on the plastic and then apply silicone to the spot sanded so it sticks. Then once its dry just push through with the needle and knock up with a few cc's of spores or LC. I saw a post from RR a few weeks ago talking about this and was dumbfounded by the fact that we go through all this trouble to mix water and karo then PC it and so on. when really all you need is a damn bottle of Apple Juice. I mean think about it. If you leave a cup of that sitting out for a day or two it grows mold. So why cant it grow mycelium. I'm trying this out myself right now and it seems to be working.


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Invisibleblood4blood
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Registered: 04/25/07
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Loc: The Valley
Re: simple LC worth it. [Re: Digital Reality]
    #7391539 - 09/10/07 11:17 AM (16 years, 4 months ago)

yea i knocked up a bottle of applejuice last night. in a couple days ill post some pics. if it works its a damn easy way to get a 1/2 gal of lc fast.


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OfflineDigital Reality
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Re: simple LC worth it. [Re: blood4blood]
    #7391598 - 09/10/07 11:29 AM (16 years, 4 months ago)

When you did your bottle did you keep its sealed and puncture the bottle through silicone or did you open it to break the vacuum seal and then inject through the top in a glove box? I know LC doesn't really need that much air and can stay in a seal container but i wasn't sure since a vacuum sealed bottle is to the extreme with that.


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Invisibleblood4blood
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Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: simple LC worth it. [Re: Digital Reality]
    #7391677 - 09/10/07 11:59 AM (16 years, 4 months ago)

what i did is i just made a sweet glovebox yesterday so ive been using it all day. i put the bottle in the glovebox took the cap off and dumped out about 1/5 of the juice into a sepreate container. then i flame sterelized a nail and made to holes in the lid and covered them both with silocone. before i put the lid back on the juice (still in the glove box) i dropped my spores in and a sterile piece of broken glass. put the lid back on. as you know one of the holes will be used as a self healing exstraction point the other hole iam going to put a filter syringe in.



i just gave it a good shake and i already have stringy mycelium allover this thing already...  :smile:


Edited by blood4blood (09/10/07 12:05 PM)


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InvisibleBlutjager
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Re: simple LC worth it. [Re: blood4blood]
    #7391694 - 09/10/07 12:03 PM (16 years, 4 months ago)

:strokebeard:


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OfflineDigital Reality
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Re: simple LC worth it. [Re: Blutjager]
    #7391712 - 09/10/07 12:09 PM (16 years, 4 months ago)

Lol you let me down jager. I saw you posted in here and got a little excited since you normally have valuable input to contribute to the forum. Instead i see a bearded smiley face thats seems to be thinking to hard. lol


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Invisibleblood4blood
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Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: simple LC worth it. [Re: Digital Reality]
    #7391820 - 09/10/07 12:44 PM (16 years, 4 months ago)

yea what does a bearded smiley face mean?


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OfflineDigital Reality
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Re: simple LC worth it. [Re: blood4blood]
    #7391914 - 09/10/07 01:11 PM (16 years, 4 months ago)

It means hes with holding information. Doom on you.:ooo:


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