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OfflineWorkmanV
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Mexicana A in Philospher's Bag*completed*
    #736237 - 07/10/02 09:12 AM (21 years, 4 months ago)

Log of sclerotia formation of Psilocybe mexicana (Strain A) in a Spore Works' Philospher's Bag.

Day 0 - 7/1/02


Uninoculated Philospher's Bag - Consistancy is like modeling clay and appears to consist mostly of rice. Syringe passed through the injection port and easily into the substrate. When the inoculate was added it flowed out of the substrate and into the space between the substrate block and the bag. I used a full 10 cc syringe since I had it available and I wanted to speed things up. A few spots of growth on the surface of the substrate were visible in 4 days at 85F and quickly spread. It reminds me of mycelium growing on the surface of an agar plate.

Day 9 - 7/10/02


Growth is looking good but is showing in only the areas where the inoculate flowed around in the bag and on top. Existing growth is healthy but no sclerotia is forming. It looks like a good time to mix the substrate.



This stuff is thick and it feels exactly like modeling clay. Its a bit difficult to mix in the bag which hasn't inflated much since inoculation. With a bit of effort I was able to get it mixed up and formed back into a mass. I didn't compact it as much as was before.

Day 14 - 7/15/02



Completely white with tiny sclerotia just starting to appear. I am moving it to room temperature inside a paper grocery sack.

Day 38 - 8/9/02





Stones looking good so far.


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Edited by Anno (10/09/02 01:04 AM)


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OfflinemrMagik
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Re: Mexicana A in Philospher's Bag [Re: Workman]
    #736611 - 07/10/02 11:17 AM (21 years, 4 months ago)

Looking good Workman, im looking forward to seeing how this turns out


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InvisibleJoshua
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Re: Mexicana A in Philospher's Bag [Re: Workman]
    #736676 - 07/10/02 11:50 AM (21 years, 4 months ago)

As I understand it the Mexicana strain was gathered by Gordon Wasson. Do you know the differences between the Mexicana strains and their ID's? I have wondered about this for a while now.

Joshua


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OfflineWorkmanV
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Re: Mexicana A in Philospher's Bag [Re: Joshua]
    #736708 - 07/10/02 12:01 PM (21 years, 4 months ago)

Psilocybe mexicana is a species. Any of the original P. mexicana strains that may have been collected by Wasson are likely lost or degenerated beyond use. The currently circulating strains are newly isolated from a wild collection. P. mexicana shouldn't be confused with mexican strains of cubensis or other species.


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InvisibleJoshua
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Re: Mexicana A in Philospher's Bag [Re: Workman]
    #736768 - 07/10/02 12:38 PM (21 years, 4 months ago)

I used the wrong word..oops, I really did know what I was talking about..I just wixed up some mords.

What is the significance of the "A" in Mexicana A?

Joshua


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OfflineWorkmanV
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Re: Mexicana A in Philospher's Bag [Re: Joshua]
    #737694 - 07/10/02 07:04 PM (21 years, 4 months ago)

The "A" is just a laboratory designation to keep it distinguished from other strains and has no other significance. The A and B strains were isolated from dried mushroom caps collected in Mexico by AIRDOG. The collection is pictured below and labeled to show the origin of each strain. The remaining caps are in cold storage for later work.



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InvisibleJoshua
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Re: Mexicana A in Philospher's Bag [Re: Workman]
    #737716 - 07/10/02 07:14 PM (21 years, 4 months ago)

I have noticed that the "A" strain is worked w/ more than the "B" strain, is there a reason for this?

Joshua


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The Shroomery Bookstore

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OfflineCaliChronic
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Re: Mexicana A in Philospher's Bag [Re: Joshua]
    #737779 - 07/10/02 07:45 PM (21 years, 4 months ago)

In reply to:

I have noticed that the "A" strain is worked w/ more than the "B" strain, is there a reason for this?



i believe the "A" strain has better sclerotia forming ability and the "B" strain has better fruiting.

Workman: Cool..we need more sclerotia grow logs...Im going to follow this post, also I'm very interested in the philosophers bags, thinking about purchasing a couple myself.


peace


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InvisibleCompiler
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Post deleted by Administrator [Re: Workman]
    #738076 - 07/11/02 01:22 AM (21 years, 4 months ago)



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InvisibleRoadkillM
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Re: Mexicana A in Philospher's Bag [Re: Compiler]
    #738886 - 07/11/02 08:54 AM (21 years, 4 months ago)

Verm ?

great job guys!!

always nice to see Workman at it!!!

I'll be watching this grow!!!


--------------------
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Invisiblemalibu76
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Post deleted by users_request [Re: Workman]
    #739106 - 07/11/02 10:11 AM (21 years, 4 months ago)



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InvisibleCompiler
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Post deleted by Administrator [Re: Roadkill]
    #739353 - 07/11/02 12:04 PM (21 years, 4 months ago)



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InvisibleGumby
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Re: Mexicana A in Philospher's Bag [Re: Compiler]
    #740170 - 07/11/02 06:51 PM (21 years, 4 months ago)

Millet?


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OfflineCaliChronic
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Re: Mexicana A in Philospher's Bag [Re: Gumby]
    #741452 - 07/12/02 10:17 AM (21 years, 4 months ago)

i think they use rye grass seed as a substrate for producing sclerotia.


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OfflineWorkmanV
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Re: Mexicana A in Philospher's Bag [Re: malibu76]
    #745387 - 07/14/02 12:00 PM (21 years, 4 months ago)

I don't know about the mixing. Maybe you shouldn't. But you are incubating at too high of temperature for quality sclerotia. I am incubating at 85F just until I see the start of sclerotia (to speed full colonization) and then lower the temperature to room temps 68-72F by bringing it into the kitchen. I just put them in a brown paper bag on a shelf and leave them alone. I have noticed that overly high temps causes excessive buildup of metabolites (yellow mycelium piss) and that can't be good.


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OfflineWorkmanV
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Re: Mexicana A in Philospher's Bag [Re: Workman]
    #751196 - 07/16/02 02:23 PM (21 years, 4 months ago)

Here is a recipe that takes advantage of the nutlike texture and taste of fresh sclerotia. I recommend weighing the amount of sclerotia used in the recipe for dosage estimation per bar.


Workman?s No Bake Chewy Chocolate Sclerotia Bars

Makes 4 dozen
Prep Time: 20 Minutes Ready in: 16 Hours 40 Minutes

Ingredients
1 cup corn syrup
3/4 cup peanut butter
1 1/2 cups semisweet chocolate chips
1 teaspoon vanilla extract
2 1/4 cups quick-cooking oats
1 3/4 cups coarsely chopped fresh sclerotia

Directions
1. In a medium saucepan over medium heat, combine the corn syrup, peanut butter and chocolate chips. Bring to a boil, stirring constantly. Continue to boil for 5 minutes.

2. Remove from heat and stir in the vanilla, oats and sclerotia. Turn onto a well greased 10x15 inch jellyroll pan or any cookie sheet with 1 inch sides. When cool enough to touch, grease your hands and pat the mixture down flat in the pan.

3. Refrigerate overnight. Let stand for 20 minutes before cutting into squares.


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InvisibleGumby
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Re: Mexicana A in Philospher's Bag [Re: Workman]
    #751897 - 07/16/02 06:50 PM (21 years, 4 months ago)

That wouldn't happen to be the recipe for the "Chocolate Starfish" bars of shrooms that are going around this area would it? A couple of friends of mine have bought these chocolate bars that have some type of psilocybin in them(shrooms or scleoita, I don't know)... 1 bar is around a level 2-3 trip and they are 25 bucks a pop.


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OfflineChromeCrow
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Re: Mexicana A in Philospher's Bag [Re: Workman]
    #752562 - 07/17/02 05:08 AM (21 years, 4 months ago)

i was wonder the potency/ quantity of sclerotia , comparing the mexicana/tamps?


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Offlinecomario
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Re: Mexicana A in Philospher's Bag [Re: Workman]
    #803925 - 08/08/02 05:09 AM (21 years, 3 months ago)

any updates? btw, i wanted to thank you for the azures spores, they already fruited, see my post in adv cult.


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OfflineWorkmanV
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Re: Mexicana A in Philospher's Bag [Re: comario]
    #807923 - 08/09/02 07:38 PM (21 years, 3 months ago)

Updated above. I thought it would be tighter if I just edited my original post to keep all the pictures together. The stones don't show up that well in the pictures but they are there in abundance. None are very big yet, most are like small peas. You can see them best from the bottom where the plastic is pressed tightly to the substrate.


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OfflineLilDon697
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Re: Mexicana A in Philospher's Bag [Re: Workman]
    #808336 - 08/10/02 05:09 AM (21 years, 3 months ago)

what are more potent . .the shrooms are the scholetera


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OfflineAddict
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Re: Mexicana A in Philospher's Bag [Re: Workman]
    #812163 - 08/12/02 05:29 AM (21 years, 3 months ago)

Hola - Workman,


I have 4 bags colonizing/fruiting of your lovely philosopher bags with mex strain a.
Three out of the four bags are fully white and forming stones heavily. One bag is 60% white and forming little sclerotia.

These bags were all innoculated on 6/28 - I was wondering would it be better to wait 3 months for harvest instead of 2?

Also why is one bag not as far along as the others - All I did was innoculate each bag with 2.5-3 ccs of spore solution and I placed them back into the shipping box. Taped it up put it in the corner of my room and left it there for 2 months, the temp in my room is around 75-78 degrees so they should be ok but sometimes when its hot it cna reach 80-85 for around 6 hours max I dunno its fucky temps in here but the crops grow fine, regardless.

Also I stole your little recipe (thank you so much for that by the way:) I was wondering do sclerotia need to be dried out first for the bars? And how much do you think 1 3/4 cups is, maybe I'll get that much from 1 bag?

And lastly whats the potency will one sclerotia bar be a good trip, how potent are these?

Thank you amigo and sorry for the excess in questions as I am very anxious at this and excited. I'd like to thank sporeworks most of all you guys are great I won these bags from your phil bag contest awhile ago....


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Offlinecomario
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Re: Mexicana A in Philospher's Bag [Re: Workman]
    #813306 - 08/12/02 03:44 PM (21 years, 3 months ago)

thanks for the update. i started four phi bags on jul1 (mexA). of the four, two did not show colonization, two are doing extremely well, one loks with large (nut size) sclerotia protruding. when to harvest? when there is no more white myc growth, only sclerotia? how early could i harvest?


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OfflineCaliChronic
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Re: Mexicana A in Philospher's Bag [Re: Workman]
    #815274 - 08/13/02 01:50 PM (21 years, 3 months ago)

wow its gettin there! cant wait to harvest my own..........mmmmmmmm....sclerotia......


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OfflineWorkmanV
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Re: Mexicana A in Philospher's Bag [Re: Addict]
    #816730 - 08/14/02 04:17 AM (21 years, 3 months ago)

I have no idea why one out of 4 of your bags is lagging.

You can harvest anytime you see enough sclerotia to satisfy yourself, but longer is better.

The no-bake cookie recipe uses fresh or rehydrated sclerotia. I don't know exactly how much sclerotia is likely from a bag, but I doubt you will get over a cup. You can 1/2 the recipe to compensate. You will need to weigh the sclerotia to estimate potency per bar. There is too much variation in weight when measuring by volume to get a good estimate. Try to get 5-10 grams fresh per bar. You also need to mix the dough/batter well to evenly spread the sclerotia and cut each bar at the same size and count the bars carefully. Good luck.

One last note: Save some of the tiny sclerotia to seed fresh jars of grain. Cleanly harvest, rinse in H2O2 and drop into sterilized jars of grass seed to perpetuate your crop at little additional cost.


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OfflineAddict
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Re: Mexicana A in Philospher's Bag [Re: Workman]
    #816806 - 08/14/02 05:15 AM (21 years, 3 months ago)

Thank you!!!!!!!

For all the info - I had no idea you could use tiny sclerotia to spawn more thats awesome!


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OfflineWorkmanV
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Re: Mexicana A in Philospher's Bag [Re: Workman]
    #922105 - 10/01/02 09:13 AM (21 years, 2 months ago)

Editing time has expired so I'll have to post the final results at the bottom of the thread.

Day 92 - 10/1/02



The bag is getting distorted with large sclerotia pushing out on all sides. Recent growth seems to be fairly rapid but I am tired of waiting. I want to see what's inside after 3 months.



A naked Philosopher's Bag. I was as careful as possible not to break off the sclerotia but some weren't even attached to the main mass. A few sclerotia were just hanging on the surface of the bag. I put the loose ones in front.



167 grams of fairly clean fresh sclerotia from the above bag. The large protruding sclerotia were easy to harvest, but the smaller ones were more troublesome. The substrate is very sticky inside and small sclerotia was time consuming to harvest. Most of the sclerotia formed on the outside edge of the substrate with very a very few small ones inside the substrate block.

Sclerotia is about 30% dry mass so the 167 grams should yield about 50 dry grams or 1.7 dry ounces.

GrowLog complete!


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Research funded by the patrons of
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Invisiblemickey_rourke
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Re: Mexicana A in Philospher's Bag [Re: Workman]
    #924098 - 10/02/02 02:22 AM (21 years, 2 months ago)

Nice! Thanks for the log Workman...


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OfflineSnobrdr311
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Re: Mexicana A in Philospher's Bag [Re: mickey_rourke]
    #924170 - 10/02/02 03:05 AM (21 years, 2 months ago)

wow, it looks awesome! any idea on how potent these sucka's are? say for example as compared to dry P. Cubensis fruits?


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Offlinecomario2
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Re: Mexicana A in Philospher's Bag [Re: mickey_rourke]
    #924942 - 10/02/02 12:42 PM (21 years, 2 months ago)

beautiful log + great results! i inoculated 2 philo bags with mex a (sporeworks!) on jul 1 and they are looking very good. i'll post yields when i open them


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Offlinegeko127
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Re: Mexicana A in Philospher's Bag [Re: Workman]
    #936204 - 10/06/02 09:54 AM (21 years, 1 month ago)

I dont know about that, I got a Ps. Mixicana print from him/her. :grin:


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Anonymous

Post deleted by Anno [Re: Workman]
    #942347 - 10/08/02 09:05 AM (21 years, 1 month ago)



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OfflinePenguin
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Re: Mexicana A in Philospher's Bag [Re: ]
    #1227449 - 01/17/03 06:00 PM (20 years, 10 months ago)

now i know what to look foward to. :laugh:


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Offlinesocratesmind
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Re: Mexicana A in Philospher's Bag [Re: ]
    #1227640 - 01/17/03 07:16 PM (20 years, 10 months ago)

It's only Ps. Mexicana and i think Ps. Tamapensis? The scletoria loving species love rye grass seed from what i hear.


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Offlineicculus84
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Re: Mexicana A in Philospher's Bag [Re: ]
    #1228201 - 01/18/03 05:14 AM (20 years, 10 months ago)

to answer a few people questioning the potency of the philosopher stones, i have heard many times they they are less potent than a cubensis fruit. how much? not sure.. but ive heard a rumored 2/3 as potent..

so.. yea.. basically im just telling yall what i've heard.
but im sure someone at sporeworks could tell yuo more precisely.


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InvisibleSixCee
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Re: Mexicana A in Philospher's Bag [Re: icculus84]
    #1228527 - 01/18/03 08:16 AM (20 years, 10 months ago)

How do you collect spores from this fruit? Was it an isolate you innoculated with?


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Offlinehappygrins
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Re: Mexicana A in Philospher's Bag [Re: SixCee]
    #1228828 - 01/18/03 10:44 AM (20 years, 10 months ago)


  >How do you collect spores from this fruit?

You would fruit them and print them the same as any other mushroom.

--happygrins :grin:


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Offlineiloveraving
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Re: Mexicana A in Philospher's Bag [Re: happygrins]
    #1230298 - 01/19/03 02:57 AM (20 years, 10 months ago)

I wish you guys still had that deal for them, I was broke cause of christmas.


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