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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
mexicana cultivation, an idea (pics)
    #735647 - 07/10/02 07:00 AM (14 years, 4 months ago)

Autoclavable widemouth polypropylene jars are filled with 200 grams of grass seed.



350 ml of water is added.



Notice how the top layer remains dry.



When all jars have been filled with water and grass seed they are dumped in an empty jar one by one to get the the wet substrate layer on top.



The top of the substrate is levelled with the bottom of a jar.



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Edited by Una (07/10/02 07:36 AM)


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OfflineBlueBus
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Registered: 04/30/02
Posts: 141
Last seen: 14 years, 1 month
Re: mexicana cultivation, an idea (pics) [Re: Una]
    #735680 - 07/10/02 07:19 AM (14 years, 4 months ago)

I've never sen grass seed used as substrate. This could be interesting.

BlueBus


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: mexicana cultivation, an idea (pics) [Re: Una]
    #735715 - 07/10/02 07:33 AM (14 years, 4 months ago)

The jar is capped with a square piece of Tyvek hold in place tightly with two rubber bands.



The jar is covered with tin foil.



And the jars are put in the pressure cooker. The 925 model can accomodate ten of these jars.



After sterilisation (1 hour) the jars are left to cool down. When they have cooled down they are put in the flow cabinet, the tinfoil is removed and any residual water on top of the tyvek is absorbed with a clean paper tissue.



The tyvek is pierced and while rotating the jar liquid inoculant(*) is divided over the substrate. About 15 ml per jar should do it. The jars can not be shaken so it?s important to wet the whole substrate with liquid.



With a hot glue gun the hole in the tyvek is shut.



Jars are now ready for incubation. The top of the jars should be covered to prevent excessive dehydration.

(*) Liquid inoculant can be prepared easily by growing out a small amount mycelium on grass seed in a bottle. Sterile water is added and this is shaken. The resulting liquid can be diluted and used for inoculations.


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InvisibleFrog31337
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Registered: 06/17/02
Posts: 779
Loc: Midwest, US
Re: mexicana cultivation, an idea (pics) [Re: Una]
    #735831 - 07/10/02 08:35 AM (14 years, 4 months ago)

What is the consistancy of the grass seed after sterilization in the canner? Does all the water get absorbed evenly? I would assume so, but just wanting to make sure.


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: mexicana cultivation, an idea (pics) [Re: Frog31337]
    #736284 - 07/10/02 11:26 AM (14 years, 4 months ago)

The grass seed on top is still a little dryer than that on the bottom. Turning the substrate after filling the jars with seed and water helps to get even moisture absorbstion.
Since the jars are not shaken and a relatively large amount of inoculant is used this doesn't really seem to matter.

Please note that this is an experiment although some good results with these jars have already been established.


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OfflinefarmersTAN
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Registered: 04/28/02
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Re: mexicana cultivation, an idea (pics) [Re: Una]
    #737208 - 07/10/02 06:05 PM (14 years, 4 months ago)

i luv tyvek, what did you cover the jars with? are you planning on extracting, or casing ?


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: mexicana cultivation, an idea (pics) [Re: farmersTAN]
    #737884 - 07/10/02 11:03 PM (14 years, 4 months ago)

The object is not to get mushrooms but to get sclerotia. The substrate need not to be cased for this.


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Anonymous

Re: mexicana cultivation, an idea (pics) [Re: Una]
    #738744 - 07/11/02 10:11 AM (14 years, 4 months ago)

Very good IDEA. Shaking ryegrass seed is a PAIN in the ass, flipping it looks real easy!!!

Nice tip on the liquid mycelium as well!!


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Invisiblecontaman
journeyman
Registered: 05/27/02
Posts: 53
Re: mexicana cultivation, an idea (pics) [Re: Una]
    #740280 - 07/11/02 09:43 PM (14 years, 4 months ago)

"(*) Liquid inoculant can be prepared easily by growing out a small amount mycelium on grass seed in a bottle. Sterile water is added and this is shaken. The resulting liquid can be diluted and used for inoculations. "

What a wonderful Idea!!!!
Thanks!


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: mexicana cultivation, an idea (pics) [Re: Una]
    #741525 - 07/12/02 12:59 PM (14 years, 4 months ago)

and of course, don?t forget to lable with strain and date. Note the hole with a bead of glue on it.



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Offlineggordon
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Registered: 06/17/02
Posts: 17
Last seen: 11 years, 2 months
Re: mexicana cultivation, an idea (pics) [Re: Una]
    #746071 - 07/14/02 07:36 PM (14 years, 4 months ago)

Sorry, just had to bump this thread because it is interesting and I dont want to see it go the way of the dodo. Please post an update when you have a chance.


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InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: mexicana cultivation, an idea (pics) [Re: ggordon]
    #753040 - 07/17/02 10:36 AM (14 years, 4 months ago)

They are developing nicely, i can't post a pic right now. Only the bottom two cm are not uncolonised. I think the substrate is a little too compact there. Doesn't really matter that much i suppose. It will serve as a water storage and will colonise eventually once the substrate mass starts shrinking.


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Amazon Shop for: ½ Pint Jars, Pressure Cooker

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