Found a Maggot in my Sclerotia Jar - Mushroom Cultivation

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javtfs
Olé!

Registered: 03/24/07
Posts: 149
Loc: Spain
Last seen: 15 years, 4 months

Re: Found a slug in my Sclerotia Jar

[Re: CureCat]
#7331852 - 08/25/07 02:08 AM (16 years, 7 months ago)

Well WTF ... I will prepare new dishes and transfer again , don´t want to risk my precious stones. I will keep this dishes anyway to see what happens. Its been two days since the slerotia is there , so I suppose that when I transfer it again it will be keep on growing in the old dish as well , toghether with the watery thing ...

The funny thing is that I have started from spores ... and now I have around 25 dishes of this.I would never have thought that i would use such a large amount ...

Well thanks for all the help and have a good weekend

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CureCat
Strangest

Registered: 04/19/06
Posts: 14,058
Loc: clawing your furniture

Re: Found a slug in my Sclerotia Jar

[Re: javtfs]
#7331884 - 08/25/07 02:28 AM (16 years, 7 months ago)

Quote:

javtfs said:
Its been two days since the slerotia is there , so I suppose that when I transfer it again it will be keep on growing in the old dish as well , toghether with the watery thing ...

Yes, it will.

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javtfs
Olé!

Registered: 03/24/07
Posts: 149
Loc: Spain
Last seen: 15 years, 4 months

Re: Found a slug in my Sclerotia Jar

[Re: CureCat]
#7336151 - 08/26/07 12:17 PM (16 years, 7 months ago)

after two days .... very poor success ... most of them present the watery / milky stain again ... so it has been a waste of time and dishes.

I might collect them from the plates , let them dry for one or two weeks and clean again and transfer again to see what happens...

Do you think it is a good idea ??

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CureCat
Strangest

Registered: 04/19/06
Posts: 14,058
Loc: clawing your furniture

Re: Found a slug in my Sclerotia Jar

[Re: javtfs]
#7342927 - 08/28/07 12:59 AM (16 years, 7 months ago)

If you are really eager to save this strain, I would take the largest sclerotia (hopefully large enough for the following procedure) and pat it dry with the paper towel. I would then drop it into a solution of 1/4 isopropyl alcohol (70%) and 3/4 water, and let it sit for 2-3 minutes, then remove the sclerotia, and in a sterile environment, pat it dry with another clean paper towel, keep it wrapped until you're ready to use it, then cross section the sclerotia, and section out a bit of tissue from the center with a scalpel or similar tool, and swiftly transfer to a new agar plate.

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javtfs
Olé!

Registered: 03/24/07
Posts: 149
Loc: Spain
Last seen: 15 years, 4 months

Re: Found a slug in my Sclerotia Jar

[Re: CureCat]
#7344055 - 08/28/07 10:14 AM (16 years, 7 months ago)

Quote:

CureCat said:
If you are really eager to save this strain, I would take the largest sclerotia (hopefully large enough for the following procedure) and pat it dry with the paper towel. I would then drop it into a solution of 1/4 isopropyl alcohol (70%) and 3/4 water, and let it sit for 2-3 minutes, then remove the sclerotia, and in a sterile environment, pat it dry with another clean paper towel, keep it wrapped until you're ready to use it, then cross section the sclerotia, and section out a bit of tissue from the center with a scalpel or similar tool, and swiftly transfer to a new agar plate.

Thanks for the answer , I will do that with the remaining pieces.

Some dishes are ok but most of them were contaminated.

I will post some pics in a couple of days when it will show a bit more of growing.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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