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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
alternitive to a innoc. loop
    #7332297 - 08/25/07 08:44 AM (16 years, 6 months ago)

jus wondering what you guys have used instead of an innoc. loop to get spores loose for making a syringe. thanks


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OfflineNibin
Getting there
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Registered: 11/29/05
Posts: 4,480
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Re: alternitive to a innoc. loop [Re: blood4blood]
    #7332324 - 08/25/07 08:53 AM (16 years, 6 months ago)

Scalpel all the way.


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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: alternitive to a innoc. loop [Re: Nibin]
    #7332341 - 08/25/07 09:02 AM (16 years, 6 months ago)

seriously? wont that damage the spores? or wreck the material the spores are on? ive thought about using this but thought that itwould be too sharp.


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OfflineRogerRabbitM
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Registered: 03/26/03
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Re: alternitive to a innoc. loop [Re: blood4blood]
    #7332351 - 08/25/07 09:08 AM (16 years, 6 months ago)

Scalpels and needles will destroy 3/4 or more of the spores. I've got a microscope picture of a slide I made by scraping the spores onto the slide with a scalpel. Almost all have major damage from the sharp edge.

If you don't have an inoculating loop, you can easily make one with a piece of wire. The idea is to gently transfer the spores with the round part of the wire so you don't cut them to shreds.
RR


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OfflineWronguy

Registered: 03/05/05
Posts: 4,450
Last seen: 12 years, 7 months
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Re: alternitive to a innoc. loop [Re: RogerRabbit]
    #7332362 - 08/25/07 09:16 AM (16 years, 6 months ago)

Don't want to use wire? You can by disposable plastic inoculation loops for about $5.00 per 10. Pretty cheap. Just make sure you get the 10UL size. I've used the smaller 1UL and it's was a pain in the ass to use it without piercing the foil.

I guess it all depends on what your preference is though.


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Invisiblemycocurious
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Registered: 02/09/07
Posts: 1,265
Re: alternitive to a innoc. loop [Re: RogerRabbit]
    #7332374 - 08/25/07 09:23 AM (16 years, 6 months ago)

I've found that cosmetic "blackhead removers" make good inoculation loops (no sharp edges) but since they cost about as much a decent inoculation loop it's more about local convenience... especially since my wife bought herself another one after her old one went missing :laugh:

best _example_ link I could find, not a product example.
http://www.zamberg.com/zb/item_281.ashx?k=GOOGLE_BASE_LIST


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:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

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figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.


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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: alternitive to a innoc. loop [Re: mycocurious]
    #7332429 - 08/25/07 09:45 AM (16 years, 5 months ago)

hey thanks guys this was all i needed for info, yea i jus read an achives thread about a scapel destroying spores from ya RR. i got sum stainless steel wiire ill giv a shot. once again thanks guys for the info


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InvisibleBlutjager
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Male

Registered: 06/11/06
Posts: 9,220
Re: alternitive to a innoc. loop [Re: blood4blood]
    #7332485 - 08/25/07 10:04 AM (16 years, 5 months ago)

I have always used a sterile scalpel blade


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OfflineNibin
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Re: alternitive to a innoc. loop [Re: Blutjager]
    #7332562 - 08/25/07 10:35 AM (16 years, 5 months ago)

I use a scalpel but I don't drag it along, I try and lift up bits of the print. Depending on the material it works better or worse though.


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Offlinexaxphaanes
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Registered: 08/08/05
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Re: alternitive to a innoc. loop [Re: Nibin]
    #7332568 - 08/25/07 10:37 AM (16 years, 5 months ago)

take a long paper clip and put tape on the end of it like electrical tape so you dont burn your hand when you heat it up.That is what i use and have had good results.


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"Anything i say is fictional"
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Offlinedeformedreality
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Registered: 06/16/07
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Re: alternitive to a innoc. loop [Re: blood4blood]
    #7332571 - 08/25/07 10:38 AM (16 years, 5 months ago)

i use my tongue then spit the spores into the jar.
>_>
<_<
no contams yet.
jk...


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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: alternitive to a innoc. loop [Re: deformedreality]
    #7332619 - 08/25/07 10:55 AM (16 years, 5 months ago)

the paper clip is a hellofa good idea. thanks xax. spitting them into the jar comes in a close 2nd though.


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InvisibleBlutjager
Inhuman
Male

Registered: 06/11/06
Posts: 9,220
Re: alternitive to a innoc. loop [Re: blood4blood]
    #7332651 - 08/25/07 11:07 AM (16 years, 5 months ago)

Quote:

blood4blood said:
Spitting them into the jar comes in a close 2nd though.





You do realize that at this very moment some poor noob is swishing his mouth out with Lysol:dielaughing:


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Offlinexaxphaanes
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Registered: 08/08/05
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Re: alternitive to a innoc. loop [Re: Blutjager]
    #7332660 - 08/25/07 11:10 AM (16 years, 5 months ago)

hahaha


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"Anything i say is fictional"
  what you should look for in manure


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InvisibleBrainiac
Rogue Scientist
Male


Registered: 04/29/06
Posts: 13,259
Loc: 與您的女朋 Flag
Re: alternitive to a innoc. loop [Re: xaxphaanes]
    #7332816 - 08/25/07 12:15 PM (16 years, 5 months ago)



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:Awesketch:

:cool: Fair is Fair :devil:


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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: alternitive to a innoc. loop [Re: Brainiac]
    #7332834 - 08/25/07 12:22 PM (16 years, 5 months ago)

Quote:

Brainiac said:
You can get 20 for 1.40 from

http://www.cynmar.com/item_detail.aspx?ItemCode=13225125




so you'd jus toss them after every use right? being as that they are plastic, you really wouldnt be able to sterlize them. its still a damn good deal though...


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