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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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How BlutJager does LC
#7320516 - 08/22/07 02:59 AM (17 years, 4 months ago) |
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BlackMach1ne
Stranger
Registered: 07/28/07
Posts: 27
Last seen: 16 years, 6 months
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Edited by BlackMach1ne (07/12/08 01:08 AM)
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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is there any safe sterile way of doing this with a piece of a print? instead of using a syringe? i got prints no syringes, i cn make a syringe but if its one less step to an lc the better.
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: How BlutJager does LC [Re: blood4blood]
#7320625 - 08/22/07 04:48 AM (17 years, 4 months ago) |
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Yes,I have done it with a print,as you said you could make a syringe and use that or you could open the bottle(In a clean glovebox of coarse)and than use something sterile to scrape some spores into it and than tighten the lid back up
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uncle_rico
my own worst enemy


Registered: 03/28/06
Posts: 2,664
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Re: How BlutJager does LC [Re: Blutjager]
#7320997 - 08/22/07 09:25 AM (17 years, 4 months ago) |
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Dear BlutJager,
Nice write-up, nice photos. Thank you.
I have bookmarked it. Between your's and Agar's ..... can't fail, bombproof instructions and images. Thanks.
Question: The baby bottle teks have nice self healing nipples but no filter port for gas exchange. I imagine the air space in the bottle provides enough? How about for a master LC that will be refriderated and used for, say, 6 months?
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nw_shroomy
NoN-stranger


Registered: 01/02/06
Posts: 1,332
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Re: How BlutJager does LC [Re: uncle_rico]
#7321034 - 08/22/07 09:40 AM (17 years, 4 months ago) |
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Edited by nw_shroomy (04/01/09 12:48 AM)
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aspore
myconaut



Registered: 07/10/07
Posts: 663
Last seen: 12 years, 4 months
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Re: How BlutJager does LC [Re: nw_shroomy]
#7321143 - 08/22/07 10:27 AM (17 years, 4 months ago) |
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Nice job! I used your(?) bottle tek for my first LC, then I went on a bottle rampage hittin up all the goodwills for their 20c bottles. I have about 25 dead baby bottles now. It's a great tek for begginers.
But now I have gallons of karo LC in jars, cause I need that damn much~! (not really)
~!aseroproe
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c l i c k m e
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Mankey


Registered: 07/30/05
Posts: 2,203
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Re: How BlutJager does LC [Re: nw_shroomy]
#7321161 - 08/22/07 10:34 AM (17 years, 4 months ago) |
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No FAE although LCs require gas exchange. Awesome job blutjager! Great to see you posting a tek! I wish I could rate you again.
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DiabloSmurf
Shake it.. Shakeit.. sugaree!



Registered: 06/25/04
Posts: 1,177
Loc: Ohio
Last seen: 16 years, 7 months
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Re: How BlutJager does LC [Re: Mankey]
#7321196 - 08/22/07 10:46 AM (17 years, 4 months ago) |
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Dude you fucking rock!!!!!
thank you so much
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ThePyschonaut52
Stranger


Registered: 04/24/07
Posts: 982
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Re: How BlutJager does LC [Re: DiabloSmurf]
#7321300 - 08/22/07 11:11 AM (17 years, 4 months ago) |
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niiice blut
-------------------- "In god we trust..."
-I guess we're screwed.
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dragnan
Curandero


Registered: 08/10/07
Posts: 11
Last seen: 4 years, 9 months
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How long roughly does it take to get the growth that I see in your bottles?
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DiabloSmurf
Shake it.. Shakeit.. sugaree!



Registered: 06/25/04
Posts: 1,177
Loc: Ohio
Last seen: 16 years, 7 months
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Re: How BlutJager does LC [Re: dragnan]
#7321356 - 08/22/07 11:25 AM (17 years, 4 months ago) |
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No more then 2 weeks if you do it right.
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Subbedhunter420
Solitary Hunter



Registered: 12/30/06
Posts: 1,501
Loc: LA/Ventura County
Last seen: 9 years, 7 months
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Re: How BlutJager does LC [Re: DiabloSmurf]
#7321393 - 08/22/07 11:41 AM (17 years, 4 months ago) |
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I just picked up some of the Turbinado cane sugar to use for LC's.
I read a while back that it is awesome for Lc's and have now knocked up 2 LC's with it but I dont like the speed of growth or the strength of the mycelium it gives. In the end, I'd stay with honey.
But have you ever used cane sugar before Blutjager? It was supposed to be a hot new LC material.
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freemynd
HyperspaceAstronaut



Registered: 06/14/07
Posts: 291
Last seen: 2 years, 5 months
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I'm assuming Karo can be used in place of the Honey...right?
-------------------- Everything I say is either hypothetical or a lie. Assume that nothing I say is true, except what I am saying right now.
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Lost Nebula
Humanaut


Registered: 05/27/07
Posts: 129
Last seen: 13 years, 10 months
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Re: How BlutJager does LC [Re: freemynd]
#7321923 - 08/22/07 02:17 PM (17 years, 4 months ago) |
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Yeah Karo can be substituted but some of us just love our honey LC's, like me. Great job Blutjager, keep it up.
Peace
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: How BlutJager does LC [Re: Lost Nebula]
#7322066 - 08/22/07 03:00 PM (17 years, 4 months ago) |
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Thanks guys,glad you like it and I hope it helps people out with their lc endeavors
Yes you can use karo and that is what was used by Agar in the 1st baby bottle tek,I have made lc out of honey,karo,malt/Dex...even maple syrup once just for a try,I have never seen anything beat honey so that is what I use and recommend but by all means if you would rather use Karo than go for it
And yea about 2 weeks in and it should be thickening up nicely,it usually goes from"Aww thats just sediment" to "WOW this lc kicks ass" in a matter of days.its stashed and I don't feel like looking for it to take a pic but somewhere around here is a bottle that is actually labeled Kick Ass lc
Edited by Blutjager (08/22/07 03:06 PM)
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uncle_rico
my own worst enemy


Registered: 03/28/06
Posts: 2,664
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Re: How BlutJager does LC [Re: Blutjager]
#7322589 - 08/22/07 05:16 PM (17 years, 4 months ago) |
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Dear BlutJager,
Thanks again.
Can someone who knows ..... describe to us the relevance of gas exchange in colonizing LC ?
--------------------
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: How BlutJager does LC [Re: uncle_rico]
#7322867 - 08/22/07 06:52 PM (17 years, 4 months ago) |
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I have seen people here make provisions for gas exchange with tubing and polyfill and all sorts of unneeded madness.
I am proof that this is unnecessary,all the air they need to grow is already in the bottle.
I'm a big fan of (Keep it simple stupid) and I look forward to making more teks in the future that spell out the easiest way to get the amount you want for the work you put in.
I'm not sure when I will get around to this cause I don't even have most of my cultivation things here and have no clue when I will be back in the game,I'm always dismantling and stashing things out of paranoia,once I do a grow again I am going to do a few more teks that make it easier on the cultivator and worthwhile their effort so that the investment of their time and money isn't wasted on just a few lonely shrooms
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nw_shroomy
NoN-stranger


Registered: 01/02/06
Posts: 1,332
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Re: How BlutJager does LC [Re: Blutjager]
#7322901 - 08/22/07 07:03 PM (17 years, 4 months ago) |
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Edited by nw_shroomy (04/01/09 12:49 AM)
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: How BlutJager does LC [Re: nw_shroomy]
#7323008 - 08/22/07 07:41 PM (17 years, 4 months ago) |
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so i got some prints on the way. as you stated earlier about just using a piece of a print for the lc that it would have to be done in a glove box (which i dont have) if i were to make a syringe out of a chunk of a print does this to have to be done in a glove box? thanks guys.
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: How BlutJager does LC [Re: blood4blood]
#7323139 - 08/22/07 08:05 PM (17 years, 4 months ago) |
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No need for fancy glovebox,A "Glove box" is for keeping the air still so contaminate spores dont get in your project,cant get much simpler than my "Upside down" aquarium still air box >>
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blood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: How BlutJager does LC [Re: Blutjager]
#7323169 - 08/22/07 08:10 PM (17 years, 4 months ago) |
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so you just do all the work from the top? and something like this would be okay for making your lc with a portion of a print? thanks for your help blut...
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: How BlutJager does LC [Re: blood4blood]
#7323330 - 08/22/07 08:40 PM (17 years, 4 months ago) |
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No you pull it to the end of the table and reach up inside of it to do your still air work
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btb103
Stranger



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Re: How BlutJager does LC [Re: Blutjager]
#7325439 - 08/23/07 11:45 AM (17 years, 4 months ago) |
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This looks like a really simple, useful tek. I'm still learning about LC (advanced mushroom cultivation in general, really) and am not sure exactly what LC is used for. If I understand correctly, using LC to inoculate is faster/better than plain spores because the colonization process has already begun and you don't have to wait for the spores to germinate.
- After the culture has been colonized (~2 weeks), you simply extract the solution and put it straight into your prepped substrate jars?
- How much LC is required to inoculate a pint or quart jar?
- After extracting the solution from the baby bottle, the silicone seal "self-heals" and the bottle can be used again, correct?
- Where can one get empty syringes? Upon purchasing them, must you PC for sterilization?
Thanks!
-------------------- In bewilderment, I realized I am the endless sea.
Edited by btb103 (08/23/07 11:47 AM)
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Neobean
Adept Mycologist


Registered: 10/07/01
Posts: 975
Loc: Canada
Last seen: 4 years, 7 months
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Re: How BlutJager does LC [Re: btb103]
#7325479 - 08/23/07 11:54 AM (17 years, 4 months ago) |
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Quote:
btb103 said: This looks like a really simple, useful tek. I'm still learning about LC (advanced mushroom cultivation in general, really) and am not sure exactly what LC is used for. If I understand correctly, using LC to inoculate is faster/better than plain spores because the colonization process has already begun and you don't have to wait for the spores to germinate.
- After the culture has been colonized (~2 weeks), you simply extract the solution and put it straight into your prepped substrate jars?
- How much LC is required to inoculate a pint or quart jar?
- After extracting the solution from the baby bottle, the silicone seal "self-heals" and the bottle can be used again, correct?
- Where can one get empty syringes? Upon purchasing them, must you PC for sterilization?
Thanks!
1- Yes 2- Very little, 1cc per jar is enough...you can put as much as you like though. 3- correct 4- You can fuck a nurse or something....or go to the local pharmacy. If they are in the package, you need not sterilize them..
-------------------- If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!
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Psilocin Dreams
PerpetuallyContaminated


Registered: 08/03/07
Posts: 187
Last seen: 6 years, 6 months
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Re: How BlutJager does LC [Re: Neobean]
#7325484 - 08/23/07 11:56 AM (17 years, 4 months ago) |
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10cc syringe blanks can be purchased at Mycopath
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: How BlutJager does LC [Re: Neobean]
#7330532 - 08/24/07 05:10 PM (17 years, 4 months ago) |
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Quote:
Neobean said:
Quote:
btb103 said: This looks like a really simple, useful tek. I'm still learning about LC (advanced mushroom cultivation in general, really) and am not sure exactly what LC is used for. If I understand correctly, using LC to inoculate is faster/better than plain spores because the colonization process has already begun and you don't have to wait for the spores to germinate.
- After the culture has been colonized (~2 weeks), you simply extract the solution and put it straight into your prepped substrate jars?
- How much LC is required to inoculate a pint or quart jar?
- After extracting the solution from the baby bottle, the silicone seal "self-heals" and the bottle can be used again, correct?
- Where can one get empty syringes? Upon purchasing them, must you PC for sterilization?
Thanks!
1- Yes 2- Very little, 1cc per jar is enough...you can put as much as you like though. 3- correct 4- You can fuck a nurse or something....or go to the local pharmacy. If they are in the package, you need not sterilize them..
If you are having trouble fucking a nurse go for pharmacy tek,they are much more readily available and usually younger and cuter as well they just lack the sexxie uniform,hell hang out at the hospital cafeteria till ya find a candy striper while your at it 
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strengthener
Stranger



Registered: 02/28/06
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Re: How BlutJager does LC [Re: Blutjager]
#7332113 - 08/25/07 06:04 AM (17 years, 4 months ago) |
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nice write up
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nw_shroomy
NoN-stranger


Registered: 01/02/06
Posts: 1,332
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Edited by nw_shroomy (04/01/09 12:53 AM)
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Wronguy

Registered: 03/05/05
Posts: 4,450
Last seen: 13 years, 6 months
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Re: How BlutJager does LC [Re: nw_shroomy]
#7332400 - 08/25/07 09:34 AM (17 years, 4 months ago) |
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A couple of observations. First of all, thank you for taking the time to put together your method of preparing liquid cultures. This will definitely come in handy for our newer folks trying this or others looking for new teks.
I have done baby bottle teks before and they all had one thing in common, their growth stalled. The reason for this was lack of gas exchange. I can see that you're combating this by using a lot of LC/spores and keeping the amount of liquid substrate down to about -250ml.
For those of us who grow liquid cultures in quart jars or larger quantities, we typically produce anywhere from 500-700ml and only have to use 1cc of spore solution and less than 1cc of LC to get things started and growth to explode. The reason for the success with smaller amounts of solution is gas exchange.
I think it's important to tell our other members the distinction between the two, so we can avoid any confusion.
Again, thank you for sharing.
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Mycofriend
Stranger
Registered: 10/23/01
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Re: How BlutJager does LC [Re: Wronguy]
#7339147 - 08/27/07 08:23 AM (17 years, 4 months ago) |
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What is your gas exchange method that doesn't allow bad guys into the LC?
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CaptainLinger
A Fungus Amongus


Registered: 05/25/07
Posts: 1,756
Last seen: 4 years, 6 months
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Re: How BlutJager does LC [Re: Mycofriend]
#7339265 - 08/27/07 09:21 AM (17 years, 4 months ago) |
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Quote:
I have done baby bottle teks before and they all had one thing in common, their growth stalled. The reason for this was lack of gas exchange.
What does it really matter if an LC "stalls"? Minuscule amounts of mycelium any way you cut it.
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Wronguy

Registered: 03/05/05
Posts: 4,450
Last seen: 13 years, 6 months
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Mycofriend, The cheapest source to provide gas exchange is poly-fill or micropore tape. I prefer to use poly-fill myself. Probably the best out there is the Whatman filters; for the simple fact that you can invert the jars when you aspirate without the fear of spilling.
CaptainLinger, The answer to your question is a simple one. If the mycelium growth stalls you have less liquid culture to work with. People grow liquid cultures to have a live culture to work with. My liquid cultures are ready for use when the mycelium constitutes 40-50% of the volume. If my growth stalled and I only had say 30% volume, my colonization times would slow down.
Having more mycelia per ml of solution means faster colonization times, which directly translates to faster harvest times. Aside from speed and efficiency is the contaminate factor. If you happen to have a bacterial infection in your substrate you want to give the mycelium the opportunity to take hold of the substrate before your competing bacteria does. Having more mycelium per ml of liquid substrate accomplishes this feat.
For those of us using liquid cultures to inoculate grains for spawn or directly inoculating substrates, the mass of mycelia is absolutely essential.
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Seengs
NACHOOOOoooo....



Registered: 11/29/05
Posts: 21
Last seen: 12 years, 1 month
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Re: How BlutJager does LC [Re: Wronguy]
#7365526 - 09/03/07 07:48 PM (17 years, 4 months ago) |
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So can you spawn bulk substrates from LC's directly? Also, it it possible to create an endless supply of LC by transferring small amounts of old LC to new bottles of LC?
-------------------- Whatcha doin?
-Chewin Chocolate.
Where'd ya get it?
-Doggie dropped it.
...Carry on...
Edited by Seengs (09/04/07 02:00 PM)
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cbiegel
A Dude

Registered: 02/19/07
Posts: 284
Last seen: 16 years, 8 months
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Re: How BlutJager does LC [Re: Seengs]
#7383281 - 09/08/07 10:52 AM (17 years, 4 months ago) |
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People absolutely knock up new LC with older ones.
My question is: Could you open the top of the bottle in a glove box to replace the stale air inside? I know it would be a risk but I'd thin the FAE would be worth it.
-------------------- Relax, don't worry, have a homebrew.
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btb103
Stranger


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Re: How BlutJager does LC [Re: blood4blood]
#7468739 - 09/29/07 11:02 PM (17 years, 3 months ago) |
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Are those plastic baby bottles? Are they okay to PC? When I first read this I assumed they were glass but I went to the store (Target) and was able to find only plastic bottles...
-------------------- In bewilderment, I realized I am the endless sea.
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MajorDick
notbeingadick


Registered: 10/14/06
Posts: 1,202
Loc: Westchestertonfieldville,...
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Re: How BlutJager does LC [Re: btb103]
#7470123 - 09/30/07 11:48 AM (17 years, 3 months ago) |
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Whudup Blut? Just thought I'd say Hi to ya.
So I've found myself wondering about G/E in baby bottles before. And I realize that there is a trade-off betwixt Gas Exchange, and easy, clean extraction. Either you can make a LC that wont spill or leak when stirred. Or a LC that can get all the Oxygen it wants as long as it stays upright.
I've only used Evenflow bottles (agars tek), and I've discovered that you can place the nipple upside down UNDER (as opposed to through) the ring lid. I'm not sure which one is better, but if you place the nipple THROUGH the lid then one can easily pull back a small area of the nipple and insert (in a clean GB) a little bit of polyfill to boost G/E if the LC stalls.
But still one must always be careful in the design of ones LC lids. Any type of filter on a LC can easily get wet with LC solution inviting in contams. Also the solution can get between parts of the lid again harboring unwanted spores or mold. I believe the "polyfill in the brake hose" trick is to avoid just those scenarios.
I have a few LCs going with old 4-hole PF lids. Solid tape on three holes, and part of a band-aid over the inoculation hole. It's not the cotton part of the band-aid just the side. Not sure if it's a great micro-filter or not. Sometimes liquid comes through when I stir/shake (no agitator), but an alcohol swab takes care of that.
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Eat The Rich
Stranger
Registered: 10/01/07
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Re: How BlutJager does LC [Re: Blutjager]
#7473653 - 10/01/07 11:49 AM (17 years, 3 months ago) |
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What is the purpose of the broken glass? I cant understand what its use would be for, and it appears more so as a danger than a benefit. Thanks so much, -Luke and Nabih.
Edited by Eat The Rich (10/01/07 11:52 AM)
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Mankey


Registered: 07/30/05
Posts: 2,203
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It is used to break up the mycelium so it is easier to get into the syringe. Quite handy.
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: How BlutJager does LC [Re: Mankey]
#7476635 - 10/02/07 08:09 AM (17 years, 3 months ago) |
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I have only used a few different kinds of baby bottles and none of them have had any problem with being pressure cooked for 20 min as the tek recommends,and yes the broken glass is to rip the mycelium into a usable size to suck into a syringe as far as letting air into the bottle in a clean glovebox I can honestly say I have never found the need to do it and most of the time have lc more than thicker than I want it but it doesn't seem like it would cause a problem as long as its under sterile conditions
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grandesign
Mushroom Madness



Registered: 08/13/07
Posts: 488
Loc:
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Re: How BlutJager does LC [Re: Blutjager]
#7476659 - 10/02/07 08:25 AM (17 years, 3 months ago) |
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thats a great tek gonna follow it soon ,, changing the subject a little hope you dont mind but im wondering if you can put mycelium from a cake into honey water to make a lc ?? is there a reason this would not work ?
-------------------- who are you ? who am i ? This shit aint real !!!
Edited by grandesign (10/02/07 08:25 AM)
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Sillicybin
Registered: 02/14/05
Posts: 2,134
Loc:
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Re: How BlutJager does LC [Re: grandesign]
#7476891 - 10/02/07 10:12 AM (17 years, 3 months ago) |
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You CAN transfer myc from a cake, but you'd have to do it G2G style, hopefully in a very sterile glovebox.
If you get ONE little nasty that transfers in with the mycelium, your LC is done.
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grandesign
Mushroom Madness



Registered: 08/13/07
Posts: 488
Loc:
Last seen: 2 years, 2 months
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Re: How BlutJager does LC [Re: Sillicybin]
#7476932 - 10/02/07 10:26 AM (17 years, 3 months ago) |
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cool thanks for that ive been wondering and read it somewhere never to see it again good job ive started bookmarking so if you could manage this with no contams thats got to be easier than collecting spores for a begginer
-------------------- who are you ? who am i ? This shit aint real !!!
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Sillicybin
Registered: 02/14/05
Posts: 2,134
Loc:
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Re: How BlutJager does LC [Re: grandesign]
#7476967 - 10/02/07 10:35 AM (17 years, 3 months ago) |
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Sure.
It requires about the same amount of skill to make your own contam-free syringes from a mushroom cap.
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grandesign
Mushroom Madness



Registered: 08/13/07
Posts: 488
Loc:
Last seen: 2 years, 2 months
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Re: How BlutJager does LC [Re: Sillicybin]
#7477008 - 10/02/07 10:46 AM (17 years, 3 months ago) |
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ok its just i have not really looked into getting spores yet but have been looking into lc and thought of killing two birds withone stone as using lc is better and stronger and if i can take a little mycelium when i birth my cakes i can be straight into lc again
-------------------- who are you ? who am i ? This shit aint real !!!
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MajorDick
notbeingadick


Registered: 10/14/06
Posts: 1,202
Loc: Westchestertonfieldville,...
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Re: How BlutJager does LC [Re: grandesign]
#7477043 - 10/02/07 10:56 AM (17 years, 3 months ago) |
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Sounds like you'd be in interested in agar work and cloning. I know I am. Best I can find on new dishes.
Strong genetic isolates are awesome.
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grandesign
Mushroom Madness



Registered: 08/13/07
Posts: 488
Loc:
Last seen: 2 years, 2 months
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Re: How BlutJager does LC [Re: MajorDick]
#7477063 - 10/02/07 11:02 AM (17 years, 3 months ago) |
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Ha im not even got a grow under my belt yet so ill look into that stuff when it comes the time to do so and that aint now lol  i am just thinking simple mindedly which keeps me out of confusion of course i do want to learn how to spore print ect this other method using myc seems easier to me cool, cheerz
-------------------- who are you ? who am i ? This shit aint real !!!
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MajorDick
notbeingadick


Registered: 10/14/06
Posts: 1,202
Loc: Westchestertonfieldville,...
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Re: How BlutJager does LC [Re: grandesign]
#7477074 - 10/02/07 11:06 AM (17 years, 3 months ago) |
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Well build a glove box and start picking out strong mycelial growths in those jars. And start collecting jars for DOZENS of LCs.
In the mean time throw some of those big words in the "search posts" page. Research is the second most fun part.
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Eat The Rich
Stranger
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Re: How BlutJager does LC [Re: Blutjager]
#7558880 - 10/25/07 11:39 AM (17 years, 2 months ago) |
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DOes anyone know the actualy proportion of honey to water to use when making an LC? I deducted from the tek that I should use 30ml Honey to 600ml Water? or rather, 1honey to 20water? is that right? Thanks So much guys!
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Quote:
Eat The Rich said:I should use 30ml Honey to 600ml Water?
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ozzyozzyozzy
Australian



Registered: 10/23/06
Posts: 545
Last seen: 4 years, 8 months
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Re: How BlutJager does LC [Re: Blutjager]
#7582789 - 11/01/07 01:12 AM (17 years, 2 months ago) |
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This style is what I used to make my honey LC. Although I did it simpler.. a few bits of honey, water, boil, shake, inoculate etc. I didn't put enough honey and I should have PC'd but there you have it. This is a good tek but you could go simpler - a doritoes salsa dip jar works perfectly as a container, just use aluminium foil over the lid for added sterility.
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Before I got a PC,I used the "doritoes jar" tek many times,sometimes it worked great,sometimes not.I love those baby bottles with sharp shards of broken glass,shake the bejezus out of it and your good to go
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stucco
Stranger
Registered: 04/24/08
Posts: 3
Last seen: 13 years, 5 months
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Re: How BlutJager does LC [Re: Blutjager]
#8882936 - 09/05/08 03:19 PM (16 years, 4 months ago) |
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what is the purpose of the shard of glass?
sorry, i'm new here.
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Hattori Hanzo
Are You Experienced?



Registered: 06/16/08
Posts: 473
Loc: South of the Mason Dixie ...
Last seen: 14 years, 1 month
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Re: How BlutJager does LC [Re: stucco]
#8883628 - 09/05/08 06:24 PM (16 years, 4 months ago) |
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to cut up the growing myc...also so you can suck it up an 18 gauge needle.
I use a marble, but me thinks, the shard of glass would be better!
H.H.
-------------------- First Grow Log
GT&TX DoubleTubs
Malabars and Hawaiians
Keep your ideals high enough to inspire you and low enough to encourage you. --unknown
 
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large_dose
Melonhead



Registered: 05/05/06
Posts: 1,346
Loc: Right in the Middle
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alright personal question for blut.
alright man, i just did the water LC tek on 4 grain jars of different strains. i injected sterile water in all the jars and shook em up a bit. when i got the myc water up into the syringes it was BLUE as hell. i always use this tek and its usually cream colored from all the myc, but this time the water in the syringes is straight up BLUE. i saw an old post of yours of how this happened to you, was the myc still just as good?
thanks L_D
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Insane Preacher
The Strange

Registered: 09/18/08
Posts: 17
Loc: The Frozen North
Last seen: 16 years, 3 months
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Re: How BlutJager does LC [Re: grandesign]
#8982263 - 09/25/08 12:48 AM (16 years, 3 months ago) |
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Quote:
grandesign said: ok its just i have not really looked into getting spores yet but have been looking into lc and thought of killing two birds withone stone as using lc is better and stronger and if i can take a little mycelium when i birth my cakes i can be straight into lc again
You could shoot sterile water into the colonized cake from a syringe, swish is a little, and suck it back up. Then use the syringe to innoc some LC bottles.
As long as its done under sterile conditions and you take care to get the needle under the dry verm before shooting or sucking fluid in your PF jar, you should do well.
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kandymews
Ms. Littols


Registered: 01/16/09 
Posts: 22
Loc: inner space
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read on the nook that using: polyfil stuffed into the nipple 3 marbles in the jar vs cutting hazard glass
works well?
squeeze the nipple with the fill in it and shake, squeezing keeps it dry. going to try this tonight and ill post some of my results as they progress.
going to be experimenting with a home mag stirrer soon, thinking of mounting a magnet to an elongated shaft of a electric motor. should do the trick!
--------------------
<i am a spiritual squitten>
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milkman59
milkman

Registered: 02/01/09
Posts: 267
Last seen: 15 years, 2 months
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Re: How BlutJager does LC [Re: Blutjager]
#11035562 - 09/10/09 05:27 PM (15 years, 4 months ago) |
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sorry for bringing up and old thread but this tek is kick ass. i got a case of these going and havent had problems at all with em. these lc's was done with agave nectar and have lasted 10 months. this is just in case any other noobs havent seen this
-------------------- milkman does wikidzons... fresh start 10/26/09
prints sent out today sorry for delay.....10/27/09
Edited by milkman59 (09/10/09 05:34 PM)
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uberdooberdan
sum dood


Registered: 10/20/10
Posts: 54
Last seen: 10 years, 4 months
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Re: How BlutJager does LC [Re: milkman59]
#13970705 - 02/15/11 09:40 PM (13 years, 10 months ago) |
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I know this is an old thread so sorry bout bringin it up!
great tek, I like it very much and look forward to trying it out but i just have on question.
after pressuring cooking and injecting from the spore syringe, where do you keep the jars and at what temperature is best?
--------------------

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Javadog
Continuing along



Registered: 05/03/10
Posts: 7,385
Loc: USA
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This is a UTSE situation...
Room temperature is good for colonization (~ 72-78 F). Do not heat the jars!
Where you store them is easy. Anywhere. On a shelf. It is good for them to get indirect daylight while colonizing.
Good luck!
JD
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes
Myco-tek.org
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monmyui
Stranger

Registered: 07/27/10
Posts: 10
Last seen: 9 years, 8 months
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Re: How BlutJager does LC [Re: Javadog]
#16590742 - 07/26/12 05:13 AM (12 years, 5 months ago) |
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Good.
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IMBiff
Learning Sponge

Registered: 01/08/15
Posts: 152
Loc: The Rabbit Hole, Wonderland
Last seen: 1 month, 30 days
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Re: How BlutJager does LC [Re: Blutjager]
#21351986 - 03/02/15 01:04 PM (9 years, 10 months ago) |
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Thanks, Blutjager! This is a super easy to follow tek. I'm a noob (hobby of only 5 months) and I'm pretty new to the forum. I'm trying your tek as I write this, so I will keep you posted. I have some South Americans I'm going to try. Is there any problem with using more than 2 cc spore solution (i.e. 4 ccs)? Reason being, I have 3 bottles and 12 ccs of inoculate. It's like the hot dog vs. bun quantity quandry... I'm grateful for your tek and for your advice! Many thanks and cheers!
-------------------- I am here to be a sponge and to soak up as much wisdom as possible from the all knowing...I am grateful for you.
For noobs like me, click here:
http://www.shroomery.org/forums/showflat.php/Number/21938807
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IMBiff
Learning Sponge

Registered: 01/08/15
Posts: 152
Loc: The Rabbit Hole, Wonderland
Last seen: 1 month, 30 days
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Re: How BlutJager does LC [Re: Blutjager]
#21352031 - 03/02/15 01:16 PM (9 years, 10 months ago) |
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I noticed the bottles constrict as they cool...

Is that normal? Thank you!
-------------------- I am here to be a sponge and to soak up as much wisdom as possible from the all knowing...I am grateful for you.
For noobs like me, click here:
http://www.shroomery.org/forums/showflat.php/Number/21938807
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