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InvisibleBlutjager
Inhuman
Male

Registered: 06/11/06
Posts: 9,220
Re: How BlutJager does LC [Re: blood4blood]
    #7323139 - 08/22/07 08:05 PM (17 years, 4 months ago)

No need for fancy glovebox,A "Glove box" is for keeping the air still so contaminate spores dont get in your project,cant get much simpler than my "Upside down" aquarium still air box >>

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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: How BlutJager does LC [Re: Blutjager]
    #7323169 - 08/22/07 08:10 PM (17 years, 4 months ago)

so you just do all the work from the top? and something like this would be okay for making your lc with a portion of a print? thanks for your help blut...

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InvisibleBlutjager
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Registered: 06/11/06
Posts: 9,220
Re: How BlutJager does LC [Re: blood4blood]
    #7323330 - 08/22/07 08:40 PM (17 years, 4 months ago)

No you pull it to the end of the table and reach up inside of it to do your still air work

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Offlinebtb103
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Registered: 02/17/07
Posts: 229
Last seen: 11 years, 8 months
Re: How BlutJager does LC [Re: Blutjager]
    #7325439 - 08/23/07 11:45 AM (17 years, 4 months ago)

This looks like a really simple, useful tek. I'm still learning about LC (advanced mushroom cultivation in general, really) and am not sure exactly what LC is used for. If I understand correctly, using LC to inoculate is faster/better than plain spores because the colonization process has already begun and you don't have to wait for the spores to germinate.

  • After the culture has been colonized (~2 weeks), you simply extract the solution and put it straight into your prepped substrate jars?
  • How much LC is required to inoculate a pint or quart jar?
  • After extracting the solution from the baby bottle, the silicone seal "self-heals" and the bottle can be used again, correct?
  • Where can one get empty syringes? Upon purchasing them, must you PC for sterilization?


Thanks!


--------------------
In bewilderment, I realized I am the endless sea.

Edited by btb103 (08/23/07 11:47 AM)

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OfflineNeobean
Adept Mycologist
Male

Registered: 10/07/01
Posts: 975
Loc: Canada
Last seen: 4 years, 7 months
Re: How BlutJager does LC [Re: btb103]
    #7325479 - 08/23/07 11:54 AM (17 years, 4 months ago)

Quote:

btb103 said:
This looks like a really simple, useful tek. I'm still learning about LC (advanced mushroom cultivation in general, really) and am not sure exactly what LC is used for. If I understand correctly, using LC to inoculate is faster/better than plain spores because the colonization process has already begun and you don't have to wait for the spores to germinate.

  • After the culture has been colonized (~2 weeks), you simply extract the solution and put it straight into your prepped substrate jars?
  • How much LC is required to inoculate a pint or quart jar?
  • After extracting the solution from the baby bottle, the silicone seal "self-heals" and the bottle can be used again, correct?
  • Where can one get empty syringes? Upon purchasing them, must you PC for sterilization?


Thanks!




1- Yes
2- Very little, 1cc per jar is enough...you can put as much as you like though.
3- correct
4- You can fuck a nurse or something....or go to the local pharmacy. If they are in the package, you need not sterilize them..


--------------------
If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!

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OfflinePsilocin Dreams
PerpetuallyContaminated


Registered: 08/03/07
Posts: 187
Last seen: 6 years, 6 months
Re: How BlutJager does LC [Re: Neobean]
    #7325484 - 08/23/07 11:56 AM (17 years, 4 months ago)

10cc syringe blanks can be purchased at Mycopath

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InvisibleBlutjager
Inhuman
Male

Registered: 06/11/06
Posts: 9,220
Re: How BlutJager does LC [Re: Neobean]
    #7330532 - 08/24/07 05:10 PM (17 years, 4 months ago)

Quote:

Neobean said:
Quote:

btb103 said:
This looks like a really simple, useful tek. I'm still learning about LC (advanced mushroom cultivation in general, really) and am not sure exactly what LC is used for. If I understand correctly, using LC to inoculate is faster/better than plain spores because the colonization process has already begun and you don't have to wait for the spores to germinate.

  • After the culture has been colonized (~2 weeks), you simply extract the solution and put it straight into your prepped substrate jars?
  • How much LC is required to inoculate a pint or quart jar?
  • After extracting the solution from the baby bottle, the silicone seal "self-heals" and the bottle can be used again, correct?
  • Where can one get empty syringes? Upon purchasing them, must you PC for sterilization?


Thanks!




1- Yes
2- Very little, 1cc per jar is enough...you can put as much as you like though.
3- correct
4- You can fuck a nurse or something....or go to the local pharmacy. If they are in the package, you need not sterilize them..



If you are having trouble fucking a nurse go for pharmacy tek,they are much more readily available and usually younger and cuter as well they just lack the sexxie uniform,hell hang out at the hospital cafeteria till ya find a candy striper while your at it :strokebeard::hehehe:

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Offlinestrengthener
Stranger
Male


Registered: 02/28/06
Posts: 119
Last seen: 16 years, 12 days
Re: How BlutJager does LC [Re: Blutjager]
    #7332113 - 08/25/07 06:04 AM (17 years, 4 months ago)

nice write up :wink:

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Invisiblenw_shroomy
NoN-stranger
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Registered: 01/02/06
Posts: 1,332
Re: How BlutJager does LC [Re: strengthener]
    #7332361 - 08/25/07 09:15 AM (17 years, 4 months ago)

.

Edited by nw_shroomy (04/01/09 12:53 AM)

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OfflineWronguy

Registered: 03/05/05
Posts: 4,450
Last seen: 13 years, 6 months
Trusted Cultivator
Re: How BlutJager does LC [Re: nw_shroomy]
    #7332400 - 08/25/07 09:34 AM (17 years, 4 months ago)

A couple of observations. First of all, thank you for taking the time to put together your method of preparing liquid cultures. This will definitely come in handy for our newer folks trying this or others looking for new teks.

I have done baby bottle teks before and they all had one thing in common, their growth stalled. The reason for this was lack of gas exchange. I can see that you're combating this by using a lot of LC/spores and keeping the amount of liquid substrate down to about -250ml.

For those of us who grow liquid cultures in quart jars or larger quantities, we typically produce anywhere from 500-700ml and only have to use 1cc of spore solution and less than 1cc of LC to get things started and growth to explode. The reason for the success with smaller amounts of solution is gas exchange.

I think it's important to tell our other members the distinction between the two, so we can avoid any confusion.

Again, thank you for sharing.

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OfflineMycofriend
Stranger
Registered: 10/23/01
Posts: 25
Last seen: 6 years, 10 months
Re: How BlutJager does LC [Re: Wronguy]
    #7339147 - 08/27/07 08:23 AM (17 years, 4 months ago)

What is your gas exchange method that doesn't allow bad guys into the LC?

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OfflineCaptainLinger
A Fungus Amongus


Registered: 05/25/07
Posts: 1,756
Last seen: 4 years, 6 months
Re: How BlutJager does LC [Re: Mycofriend]
    #7339265 - 08/27/07 09:21 AM (17 years, 4 months ago)

Quote:

I have done baby bottle teks before and they all had one thing in common, their growth stalled. The reason for this was lack of gas exchange.




What does it really matter if an LC "stalls"? Minuscule amounts of mycelium any way you cut it.

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OfflineWronguy

Registered: 03/05/05
Posts: 4,450
Last seen: 13 years, 6 months
Trusted Cultivator
Re: How BlutJager does LC [Re: CaptainLinger]
    #7339588 - 08/27/07 10:54 AM (17 years, 4 months ago)

Mycofriend,
The cheapest source to provide gas exchange is poly-fill or micropore tape. I prefer to use poly-fill myself. Probably the best out there is the Whatman filters; for the simple fact that you can invert the jars when you aspirate without the fear of spilling.

CaptainLinger,
The answer to your question is a simple one. If the mycelium growth stalls you have less liquid culture to work with. People grow liquid cultures to have a live culture to work with. My liquid cultures are ready for use when the mycelium constitutes 40-50% of the volume. If my growth stalled and I only had say 30% volume, my colonization times would slow down.

Having more mycelia per ml of solution means faster colonization times, which directly translates to faster harvest times. Aside from speed and efficiency is the contaminate factor. If you happen to have a bacterial infection in your substrate you want to give the mycelium the opportunity to take hold of the substrate before your competing bacteria does. Having more mycelium per ml of liquid substrate accomplishes this feat.

For those of us using liquid cultures to inoculate grains for spawn or directly inoculating substrates, the mass of mycelia is absolutely essential.

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OfflineSeengs
NACHOOOOoooo....
Male


Registered: 11/29/05
Posts: 21
Last seen: 12 years, 1 month
Re: How BlutJager does LC [Re: Wronguy]
    #7365526 - 09/03/07 07:48 PM (17 years, 4 months ago)

So can you spawn bulk substrates from LC's directly? Also, it it possible to create an endless supply of LC by transferring small amounts of old LC to new bottles of LC?


--------------------
Whatcha doin?
-Chewin Chocolate.
Where'd ya get it?
-Doggie dropped it.
...Carry on...

Edited by Seengs (09/04/07 02:00 PM)

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Offlinecbiegel
A Dude

Registered: 02/19/07
Posts: 284
Last seen: 16 years, 8 months
Re: How BlutJager does LC [Re: Seengs]
    #7383281 - 09/08/07 10:52 AM (17 years, 4 months ago)

People absolutely knock up new LC with older ones.

My question is: Could you open the top of the bottle in a glove box to replace the stale air inside? I know it would be a risk but I'd thin the FAE would be worth it.


--------------------
Relax, don't worry, have a homebrew.

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Offlinebtb103
Stranger
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Registered: 02/17/07
Posts: 229
Last seen: 11 years, 8 months
Re: How BlutJager does LC [Re: blood4blood]
    #7468739 - 09/29/07 11:02 PM (17 years, 3 months ago)

Are those plastic baby bottles? Are they okay to PC? When I first read this I assumed they were glass but I went to the store (Target) and was able to find only plastic bottles...


--------------------
In bewilderment, I realized I am the endless sea.

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InvisibleMajorDick
notbeingadick
Male User Gallery

Registered: 10/14/06
Posts: 1,202
Loc: Westchestertonfieldville,... Flag
Re: How BlutJager does LC [Re: btb103]
    #7470123 - 09/30/07 11:48 AM (17 years, 3 months ago)

Whudup Blut? Just thought I'd say Hi to ya.



So I've found myself wondering about G/E in baby bottles before. And I realize that there is a trade-off betwixt Gas Exchange, and easy, clean extraction. Either you can make a LC that wont spill or leak when stirred. Or a LC that can get all the Oxygen it wants as long as it stays upright.

I've only used Evenflow bottles (agars tek), and I've discovered that you can place the nipple upside down UNDER (as opposed to through) the ring lid. I'm not sure which one is better, but if you place the nipple THROUGH the lid then one can easily pull back a small area of the nipple and insert (in a clean GB) a little bit of polyfill to boost G/E if the LC stalls.

But still one must always be careful in the design of ones LC lids. Any type of filter on a LC can easily get wet with LC solution inviting in contams. Also the solution can get between parts of the lid again harboring unwanted spores or mold. I believe the "polyfill in the brake hose" trick is to avoid just those scenarios.


I have a few LCs going with old 4-hole PF lids. Solid tape on three holes, and part of a band-aid over the inoculation hole. It's not the cotton part of the band-aid just the side. Not sure if it's a great micro-filter or not. Sometimes liquid comes through when I stir/shake (no agitator), but an alcohol swab takes care of that.

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OfflineEat The Rich
Stranger
Registered: 10/01/07
Posts: 23
Last seen: 16 years, 2 months
Re: How BlutJager does LC [Re: Blutjager]
    #7473653 - 10/01/07 11:49 AM (17 years, 3 months ago)

What is the purpose of the broken glass? I cant understand what its use would be for, and it appears more so as a danger than a benefit.
Thanks so much,
-Luke and Nabih.

Edited by Eat The Rich (10/01/07 11:52 AM)

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InvisibleMankey
Male

Registered: 07/30/05
Posts: 2,203
Re: How BlutJager does LC [Re: Eat The Rich]
    #7474732 - 10/01/07 05:14 PM (17 years, 3 months ago)

It is used to break up the mycelium so it is easier to get into the syringe. Quite handy.

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InvisibleBlutjager
Inhuman
Male

Registered: 06/11/06
Posts: 9,220
Re: How BlutJager does LC [Re: Mankey]
    #7476635 - 10/02/07 08:09 AM (17 years, 3 months ago)

I have only used a few different kinds of baby bottles and none of them have had any problem with being pressure cooked for 20 min as the tek recommends,and yes the broken glass is to rip the mycelium into a usable size to suck into a syringe as far as letting air into the bottle in a clean glovebox I can honestly say I have never found the need to do it and most of the time have lc more than thicker than I want it but it doesn't seem like it would cause a problem as long as its under sterile conditions

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