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Offlinerodfarva
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Registered: 07/31/07
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Last seen: 7 years, 1 month
Reactions to first grow *DELETED*
    #7316061 - 08/20/07 11:00 PM (16 years, 7 months ago)

Post deleted by rodfarva

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Offlineclover606
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Registered: 08/13/07
Posts: 656
Last seen: 10 years, 6 months
Re: Reactions to first grow [Re: rodfarva]
    #7316139 - 08/20/07 11:19 PM (16 years, 7 months ago)

definatly cobweb mold. all over it.

lol jk, they dont look bad to me.
i think your in for a good time in a month or so


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grassman said:

I remember being in DARE when i was much younger and some of the stories they would tell you are not only ridiculous, but completely untrue. One story was that a woman was on LSD and thought her infant was a turkey so she baked it in the oven. Now I look back and think thats hilarious, but at the time I guess it scared me.

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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: Reactions to first grow [Re: clover606]
    #7316156 - 08/20/07 11:25 PM (16 years, 7 months ago)

yup they look good, are your holes uncovered? you want adequate gas exchange while they are colonizing. 3 weeks is the max they shud take to fully colonize so ur good . jus dnt peek on them so often and go bac in a week or so and youll be hapily surprised

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Offlinerodfarva
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Re: Reactions to first grow [Re: blood4blood]
    #7316243 - 08/21/07 12:04 AM (16 years, 7 months ago)

lol im looking forward to a month or so. Ill stop peeking after 5 more days or so, if those dark spots grow, im going to nuke them... ill try posting a pic...




notice the black dots.. the other suspected contam  is a dark circle that has mosture droplets on the glass. could be just moisture, prolly not :frown:


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Offlinerodfarva
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Re: Reactions to first grow [Re: rodfarva]
    #7316300 - 08/21/07 12:31 AM (16 years, 7 months ago)

comments?


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Offlinerodfarva
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Registered: 07/31/07
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Re: Reactions to first grow [Re: rodfarva]
    #7316789 - 08/21/07 05:26 AM (16 years, 7 months ago)

... and yes thx the holes are open like i want my pupils to b :ooo:


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Offlineexagram
Phear the shroom
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Registered: 02/28/07
Posts: 302
Last seen: 15 years, 10 months
Re: Reactions to first grow [Re: rodfarva]
    #7316797 - 08/21/07 05:43 AM (16 years, 7 months ago)

Quote:

rodfarva said:
I isopropled the needle after every hole, wore gloves, and could barely breath b/c of all the isopropyl i sprayed around. I put them in my fc and kept the heat around 75.




You might want to go easy on the isopropyl. You don't want it to end up in your jars through the inoculation holes. I find it's best to spray the room you're going to use the day before the actual inoculation. A safer way to sterilise the needle after inoculation is to use a lighter or other flame - just make sure you get it to red-hot all the way along the shaft. Also, you only need to sterilise after every jar, not every hole on each jar (I assume you have more than one in each).

If you want to speed up colonisation, you can boost the temperature to anywhere up to 81F, though it won't make a massive difference (maybe a day or three).

Most of your jars are looking healthy. Good luck!


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Raoul Duke: What Leary took down with him was the central illusion of a whole lifestyle that he helped create - a generation of permanent cripples, failed seekers, who never understood the essential old-mystic fallacy of the acid culture: the desperate assumption that somebody, or at least some force, is tending the light at the end of the tunnel.

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Offlineveda_sticks
Cultivator
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Registered: 07/29/07
Posts: 14,191
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Re: Reactions to first grow [Re: exagram]
    #7316810 - 08/21/07 06:09 AM (16 years, 7 months ago)

is everybody absolutly sure there are no contams, im new to this too butnone of my jars are like this, they are all pure white, there are some worrying grey patches??

im new to this to so i could be wrong.

Once they are fully colinised should be completly white and smell nice n mushroomy


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PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Offlinedirtracer24
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Registered: 03/10/07
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Re: Reactions to first grow [Re: veda_sticks]
    #7316954 - 08/21/07 08:15 AM (16 years, 7 months ago)

looks like straight up contams to me brother sorry to be the bearer of badnews but start over and practice better sterile teks on more than just the needle make a still air box and quit breathing your breathe will get ya everytime


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why do we park in driveways?and drive in parkways?

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Offlinemoon_glue
Orwell's Post9/11 Era
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Registered: 01/20/07
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Re: Reactions to first grow [Re: dirtracer24]
    #7316963 - 08/21/07 08:22 AM (16 years, 7 months ago)

most people fuck up their first attempts. i fucked up my first 4 months of attempts.

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Offlinelightningbolt1
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Registered: 04/13/07
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Re: Reactions to first grow [Re: moon_glue]
    #7317530 - 08/21/07 11:23 AM (16 years, 7 months ago)

those dark spots are contams


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In the land of the dark, the ship of the sun is driven by the Grateful Dead

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InvisibleBridgeburner
Not spiritual at all.
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Re: Reactions to first grow [Re: lightningbolt1]
    #7317551 - 08/21/07 11:28 AM (16 years, 7 months ago)

yup, looks like they're fubar.


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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: Reactions to first grow [Re: Bridgeburner]
    #7317641 - 08/21/07 11:55 AM (16 years, 7 months ago)

they sorta jus look like the wet spots from the spore solution

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InvisibleBridgeburner
Not spiritual at all.
Male


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Posts: 20,010
Re: Reactions to first grow [Re: blood4blood]
    #7317779 - 08/21/07 12:41 PM (16 years, 7 months ago)

spore solution doesn't leave wet spots, which is a contam anyway.


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Offlinekranked
it'smushtastic!!


Registered: 08/31/04
Posts: 106
Last seen: 16 years, 7 months
Re: Reactions to first grow [Re: Bridgeburner]
    #7317813 - 08/21/07 12:51 PM (16 years, 7 months ago)

I really don't know what you guys were all doing when you started out, something funky I'm sure. My first ten grows were pf-teks with 15 minute boil sterilizations, and not a single contam.


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"When it went off in New Mexico, that first atomic bomb, we thought of Alfred Nobel and his hope, his vain hope, that dynamite would put an end to all war"

-Robert Oppenheimer 1945

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Invisiblemycocurious
Mike O. Kuerias
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Registered: 02/09/07
Posts: 1,265
Re: Reactions to first grow [Re: kranked]
    #7317891 - 08/21/07 01:20 PM (16 years, 7 months ago)

The problem, generally is not in how the substrate was sterilized or if it was sterilized enough, it's generally in the sterility of the inoculation procedure.

#1 - Sterilize your inoculation location, top to bottom.
#2 - Place equipment into clean room.
#3 - Spray air sanitizer (oust is good) into the air, thoroughly.
#4 - Go start a wash cycle with a pair of scrubs. Hell I have a fav pair of pajama's I wear.
#5 - Jump in the shower, brush your teeth, dry your hair, shave, etc. BE CLEAN.
#6 - Hair net, surgical (not dust) mask, tyvek arm sleeves, rubber gloves.

And even then, I'm constantly wiping everything down with more rubbing alcohol as I go, flame sterilizing my needle (until red hot) between inoculating every single jar, without exception.

-----------------------------
These are the steps where I think people really skimp out and get into the most trouble, in my humble opinion.


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:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

- How Myco-Curious Prepares Coir & Compost Substrates
- How Myco-Curious Builds A Bulk Humidifier
- How Myco-Curious Builds An Automated Greenhouse
------------------------------------
figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.

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Offlinerodfarva
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Registered: 07/31/07
Posts: 4,982
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Re: Reactions to first grow *DELETED* [Re: mycocurious]
    #7318052 - 08/21/07 02:14 PM (16 years, 7 months ago)

Post deleted by rodfarva

Reason for deletion: .



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Invisiblemycocurious
Mike O. Kuerias
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Registered: 02/09/07
Posts: 1,265
Re: Reactions to first grow [Re: rodfarva]
    #7318176 - 08/21/07 02:44 PM (16 years, 7 months ago)

I think liquid cultures really over-complicates the process for most new hobbyist and it's another entry point for a contaminate along the way. That being said, it can turn a little spore solution into a lot of inoculate when done correctly.

One piece of advice on that though, when using LC, you cannot "load it up" to make up for lost time. Doing so invariably screws up your moisture content of the jar and remember that syrupy solution of a LC is a contaminate MAGNET once inside your jar. Ideally you would use it just as sparingly as you would spore solution.


--------------------
:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

- How Myco-Curious Prepares Coir & Compost Substrates
- How Myco-Curious Builds A Bulk Humidifier
- How Myco-Curious Builds An Automated Greenhouse
------------------------------------
figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.

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Offlinejaybean77
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Registered: 07/04/07
Posts: 46
Last seen: 8 years, 1 month
Re: Reactions to first grow [Re: rodfarva]
    #7318199 - 08/21/07 02:49 PM (16 years, 7 months ago)

@rodfarva
what do you mean by lc'ing one and loading it w/ inoculant?
what is "it"?
do you mean making some liquid culture and then overdoing it inoculating the jars?
too much LC can ruin a jar because all that water can encourage bacteria to set in. bacteria pretty much runs light speed compared to Psilocybe, if you give them the right conditions. so ive heard, i could be wrong. on a similar note ive heard that too much spawn (spawning to bulk) is almost pointless because you reach a point of diminishing returns. i assume its the same w/ inoculant only worse because overdoing it actually can be bad.
you CAN adjust the water ratio of your substrate ahead of time to avoid this but i dont see the point. if 3cc's of spore solution is sufficient to inoculate a 3 lb bag of substrate, than anything more than 1-3cc's of LC would be over kill. word is that LC rips through substrate as is even w/ conservative amounts.
BUT
i think even newbs (me included) should try a few cc's of spore solution on making a LC everytime. why not? LC goes a LONG way and it can be expanded into gallons if you want. only problem is its pretty hard to detect a contam in a LC. and whats the point of having loads of contaminated culture right? damnit fuckin' germs.......
but if you can get it working then you are golden. always worth a shot. (no pun intended)
good luck :smile:

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Offlinerodfarva
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Re: Reactions to first grow [Re: jaybean77]
    #7318556 - 08/21/07 04:24 PM (16 years, 7 months ago)

Yeah thanks for the heads up, i hadnt thought about those points. Maybe its not for me i have enough innoculant coming and I only want to Make one solid go, perhaps a year supply which for me would include one or two gormet meals a month for me and one or two friends. From what i read a casing the size im planing will about do it. ( 12" by 20")


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