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Offlinebrodus24
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Amazing Isolate EATS contams!!!
    #7301685 - 08/16/07 04:53 PM (16 years, 7 months ago)

This is the coolest thing that I've happened upon in this hobby, next to my shroom-on-shroom grow.

I have a PB (Thai Pink Buffalo) strain that I isolated from the largest, fastest growing fruit. This was a veil/cap tissue isolation in bacteriostatic water.

Anyhow...its INSANE...I've had two WBS jars now that initially contaminated a bit...in both cases the PB myc overtook the contams, spitting out a great deal of metabolites (myco-piss) along the way. First jar had pink mold...this current jar had green/gray trich. that was evident upon shaking...now its almost all gone and covered with healthy white Mycelium!

I'm taking photos to post, because its unbelievable...

FYI, I had other jars that had no contams. These jars were jars that had initially been inoculated and for whatever reason, they never spawned mycelium....so I tossed in some PB-isolate liquid, and within a couple days had mycelium...then some contams and mycelium...and now ONLY mycelium!

I'm going to clone this isolate forever!

ON a side note, I just watched a YouTube video with Paul Stamets explaining mushroom-based bio-remediation, and if mycelium can eat and digest oil slicks, it makes sense that they can overtake contams.

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OfflineDr_T
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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
    #7301734 - 08/16/07 05:12 PM (16 years, 7 months ago)

Nice!

(Could I get a sample? Please?)


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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
    #7301757 - 08/16/07 05:22 PM (16 years, 7 months ago)

too bad that its frowned on to send myc lc through the mail to other members.. id probally even pay for it... COUGH COUGH COUGH


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Offlinebrodus24
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Re: Amazing Isolate EATS contams!!! [Re: deformedreality]
    #7301842 - 08/16/07 05:59 PM (16 years, 7 months ago)

Yeah, its going to stay in my collection forever I think :smile:

I'm slowly going all-clone/isolate...this is how some people have their WBS jars colonize in 14-18 days, while others take 30+.

I've had cakes of the same multi-spore produce shat, or produce huge.  Its all because I didn't have an isolate.

I think my new strategy for each new strain is:
1. FIRST test the strain on BRF...isolate the best, make an LC of that
2. THEN grow out the iso-LC on WBS to poo/straw.
3. Repeat forever until I have 5 or so strains that represent 5 or so different characteristics I look for (size, colonization speed, etc.)

In this manner, I'm never out on a shitty casing, slug-slow WBS colonization, or sub-par flush.

I took some photos, but the light doesn't show the digested trich very well...I'll try to find another way to photograph.

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Offlinexaxphaanes
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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
    #7301898 - 08/16/07 06:12 PM (16 years, 7 months ago)

not to rain on your parade but all that happened is the mycelium covered up the contam, the contam is still there if you spawn i almost guarantee that it will contam i have had this happen a few times.


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  what you should look for in manure

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Offlinedeformedreality
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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
    #7301924 - 08/16/07 06:20 PM (16 years, 7 months ago)

well im just glad i dont have to rely on a myc that can get rid of contams.. hell i can basically wipe my ass with cakes and they wont contam..


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Offlinebrodus24
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Re: Amazing Isolate EATS contams!!! [Re: xaxphaanes]
    #7302097 - 08/16/07 07:16 PM (16 years, 7 months ago)

Actually, the first jar is already cased in CC and 7 days done--just looked at it: zero contams, just a ton of fluffy white mycelium.

I'll post results on everything, but its not at all unlikely that the contams we overtaken. Ref: myco-remediation.

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Offlinexaxphaanes
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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
    #7302181 - 08/16/07 07:38 PM (16 years, 7 months ago)

ok well gl out of maybe 100 jars that i tried ended up with contams.


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"Anything i say is fictional"
  what you should look for in manure

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Invisiblejeetered
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Re: Amazing Isolate EATS contams!!! [Re: xaxphaanes]
    #7302244 - 08/16/07 07:59 PM (16 years, 7 months ago)

ok i've searched every term possible on youtube for the video you refer to... don't find it. can we get a link? i've read it but not seen it.

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Re: Amazing Isolate EATS contams!!! [Re: jeetered]
    #7302264 - 08/16/07 08:04 PM (16 years, 7 months ago)


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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
    #7302280 - 08/16/07 08:11 PM (16 years, 7 months ago)

the question is, are the oysters still edible...


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Offlinexaxphaanes
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Re: Amazing Isolate EATS contams!!! [Re: jeetered]
    #7302302 - 08/16/07 08:16 PM (16 years, 7 months ago)

yes that video is pretty good..also check out anastomosis which is basically the joining of two diff mycelia i.e green mold/cobweb etc etc


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"Anything i say is fictional"
  what you should look for in manure

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Offlineimplee
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Re: Amazing Isolate EATS contams!!! [Re: xaxphaanes]
    #7302363 - 08/16/07 08:32 PM (16 years, 7 months ago)

Mushrooms are hardcore!

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Offlinebrodus24
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Re: Amazing Isolate EATS contams!!! [Re: implee]
    #7305056 - 08/17/07 01:56 PM (16 years, 7 months ago)

Just search Paul Stamets on YouTube...there's a lot of good stuff...he's my hero

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Offlinebrodus24
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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
    #7386501 - 09/09/07 01:16 AM (16 years, 6 months ago)

FYI, this casing is fruiting now and I have an insane amount of pins...I'll post photos.

You won't believe it.

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OfflineThe shroomy 1
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Re: Amazing Isolate EATS contams!!! [Re: deformedreality]
    #7386514 - 09/09/07 01:25 AM (16 years, 6 months ago)

Quote:

deformedreality said:
well im just glad i dont have to rely on a myc that can get rid of contams.. hell i can basically wipe my ass with cakes and they wont contam..




SHHHHHHHHHHHHHH!!!!!!!!!!! The contamination GODS will hear you!!


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AMU Q&A thread.

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OfflineeVenom
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Re: Amazing Isolate EATS contams!!! [Re: The shroomy 1]
    #7388115 - 09/09/07 12:59 PM (16 years, 6 months ago)

come one almost a month and still no pictures I'm dying to see them


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eVenom

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InvisibleWeed_Indicated
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Re: Amazing Isolate EATS contams!!! [Re: deformedreality]
    #7388254 - 09/09/07 01:51 PM (16 years, 6 months ago)

Quote:

deformedreality said:
COUGH COUGH COUGH




LOL


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FrankHorrigan WBS Prep
Magesh's Rye Tek
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Liquid Culture Basics
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Offlineveda_sticks
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Re: Amazing Isolate EATS contams!!! [Re: Weed_Indicated]
    #7388296 - 09/09/07 02:06 PM (16 years, 6 months ago)

interesting


--------------------

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Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
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Offlinebrodus24
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Re: Amazing Isolate EATS contams!!! [Re: veda_sticks]
    #7393212 - 09/10/07 06:50 PM (16 years, 6 months ago)

Here are photos--was waiting to get my good camera back, as the cellphone just doesn't do justice.

Got some early poppers, but below them a very dense pinset.

Beware: these are LARGE:







Edited by brodus24 (09/10/07 06:51 PM)

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Offlinebrodus24
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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
    #7393217 - 09/10/07 06:52 PM (16 years, 6 months ago)

Hmm... I guess the were auto-resized...

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Invisibleorchidfanatic
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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
    #7393230 - 09/10/07 06:56 PM (16 years, 6 months ago)

pretty .. that seems super deep for a mono tub .. ? how deep is that substrate? 16 inches?

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OfflineeVenom
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Re: Amazing Isolate EATS contams!!! [Re: orchidfanatic]
    #7393606 - 09/10/07 08:17 PM (16 years, 6 months ago)

that don't look like a clone to me! but maybe that you didn't put no casing layer is what made it look like that! or did you???

maybe time will tell!!good luck!

and post some more pics when they are mature!


--------------------
eVenom

"Some of us like a nice buzz, while others want to climb a screaming volcano naked to meet god."
RR

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Offlinegerbletits
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Re: Amazing Isolate EATS contams!!! [Re: eVenom]
    #7393644 - 09/10/07 08:28 PM (16 years, 6 months ago)

a clone looks just like any other mushroom...?

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OfflineeVenom
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Re: Amazing Isolate EATS contams!!! [Re: gerbletits]
    #7393822 - 09/10/07 08:55 PM (16 years, 6 months ago)

yes but the thing is that when you work with a clone almost always there is an even pinset and and the shrooms all look the same!and that's not the case in here!

when i find a good comparison ill post it!


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eVenom

"Some of us like a nice buzz, while others want to climb a screaming volcano naked to meet god."
RR

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Invisibledysphoria
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Re: Amazing Isolate EATS contams!!! [Re: eVenom]
    #7394012 - 09/10/07 09:33 PM (16 years, 6 months ago)

no man, a clone is a clone, and that can be anything.
i could start cloning for genetic mutations, or even NON-mushroom producing strains.
this clone could be of a shitty pinset clone, but one that eats contaminations nontheless.


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InvisiblePeterthinks
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Re: Amazing Isolate EATS contams!!! [Re: gerbletits]
    #7394016 - 09/10/07 09:35 PM (16 years, 6 months ago)

A clone looks just like every other mushroom that looks like itself.
By the way what contaminates did it eat?
cobweb?trich?penicillin?

:jester:


--------------------
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Edited by Peterthinks (09/10/07 09:38 PM)

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OfflineThe shroomy 1
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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
    #7394045 - 09/10/07 09:39 PM (16 years, 6 months ago)

I would start a second generation clone with the nicest fruit of this batch. If indeed it does eat contamination and is able to somewhat fruit, I would be pulling out the petri dishes and start getting to work!


--------------------


AMU Q&A thread.

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OfflineRogerRabbitM
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Re: Amazing Isolate EATS contams!!! [Re: The shroomy 1]
    #7394074 - 09/10/07 09:45 PM (16 years, 6 months ago)

Very often, the damaged, blue-gray mycelium that results from shaking is mistaken for contaminant molds.
RR


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Offlinexaxphaanes
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Re: Amazing Isolate EATS contams!!! [Re: RogerRabbit]
    #7394237 - 09/10/07 10:27 PM (16 years, 6 months ago)

I dont understand how this would work.Wouldnt they just join together? isnt cube mycelium compatible with trich or cobweb?


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"Anything i say is fictional"
  what you should look for in manure

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OfflineeVenom
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Re: Amazing Isolate EATS contams!!! [Re: dysphoria]
    #7394599 - 09/11/07 12:08 AM (16 years, 6 months ago)

Quote:

dysphoria said:
no man, a clone is a clone, and that can be anything.
i could start cloning for genetic mutations, or even NON-mushroom producing strains.
this clone could be of a shitty pinset clone, but one that eats contaminations nontheless.




How is this possible I know that you could do an isolate of a non-mushroom producing substrain but how to do a shitty pinset isolate,,, either it pins or it don't and it will grow at the same rate if provided the same conditions.

but how will you isolate a substrain that sometimes it grows, some times it don't grow and at different rates?

I really would love to learn more about how is this possible.


--------------------
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OfflineWalter1496211
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Re: Amazing Isolate EATS contams!!! [Re: brodus24]
    #7394658 - 09/11/07 12:30 AM (16 years, 6 months ago)

That is definitly amazing


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Re: Amazing Isolate EATS contams!!! [Re: eVenom]
    #7394684 - 09/11/07 12:41 AM (16 years, 6 months ago)

Quote:

eVenom said:I know that you could do an isolate of a non-mushroom producing substrain but how to do a shitty pinset isolate,,, either it pins or it don't and it will grow at the same rate if provided the same conditions.



I'm no expert but it seems to me that when you clone mushroom tissue you're in fact cloning mycelium. And it's quite possible that one myc has a genetic trait which produces its fruit evenly all at the same time while another has a trait which produces its fruit unevenly.

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OfflineRogerRabbitM
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Re: Amazing Isolate EATS contams!!! [Re: roquet]
    #7395320 - 09/11/07 06:54 AM (16 years, 6 months ago)

Quote:

Wouldnt they just join together? isnt cube mycelium compatible with trich or cobweb?




Cube mycelium will not join with trich or cobweb. Anastomosis is the pairing of dikaryotic mycelium with other dikaryotic mycelium. In other words, combining strains, but not species. For example, Penis Envy could combine with cambodian cubensis via anastomosis, but neither could combine with oyster, shiitake, or mold.
RR


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