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Offlinezdev
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How much LC should I use per jar?
    #7279714 - 08/10/07 12:56 AM (13 years, 9 months ago)

I'm going to make some LC jars using this tek and use them for my next grow, I have got a 10cc spore syringe, so that means I can make 10 of these LC jars, more than enough to knock up the 12 jars I have total.

Since I'm going to have more LC than I need, should I just use more for each jar? He says to use 2cc per jar, but if I have enough for let's say, 20cc per jar, should I go ahead and use 20cc per jar?


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Offlinelipan
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Re: How much LC should I use per jar? [Re: zdev]
    #7279731 - 08/10/07 01:00 AM (13 years, 9 months ago)

more cultures means more solution. You don't need to over knock up jars. Just think of it as having a huge supply of cutlture.


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Offlinekidaihuan
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Re: How much LC should I use per jar? [Re: lipan]
    #7279898 - 08/10/07 01:49 AM (13 years, 9 months ago)

Wouldn't more culture in each jar mean a better coverage, faster colonization and as a result a reduced risk of contamination?

I think it would be ok, but you would have to accommodate for this when you make your jars. If you're going to use 20cc of culture, then put 20cc less water in the jar before you pressure cook it.


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Offlinelipan
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Re: How much LC should I use per jar? [Re: kidaihuan]
    #7280020 - 08/10/07 02:18 AM (13 years, 9 months ago)

wouldnt make a profound difference. It means you have to dip into your culture more and more.


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Offlinejash420
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Re: How much LC should I use per jar? [Re: lipan]
    #7280289 - 08/10/07 04:46 AM (13 years, 9 months ago)

I would not use even 2ml unless you are talking about spread through 4 different holes in a PF style jar, I've overused LC and it fucked alot of shit up as the moisture content in subs needs to be just so so..


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Offlinefiggusfiddus
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Re: How much LC should I use per jar? [Re: jash420]
    #7280328 - 08/10/07 05:37 AM (13 years, 9 months ago)

Yep, moisture content is the reason here. Don't do more than 1cc per entry point. I'd say about half that is good.

When I use extra inoculant like that, I do like to wiggle the needle a little inside the jar to distribute the inoculant over a wider area as I inject. It seems to work well, since it both prevents puddling and gives you a better head start once your culture takes off.


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Offlinezdev
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Re: How much LC should I use per jar? [Re: figgusfiddus]
    #7280590 - 08/10/07 09:56 AM (13 years, 9 months ago)

Instead of over inoculating the 4 points, if you have tons of LC why not just inoculate more than 4 points? Like you know, a couple on the bottom, a couple near the top, a couple in the middle, etc.


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Offlinefiggusfiddus
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Re: How much LC should I use per jar? [Re: zdev]
    #7280595 - 08/10/07 09:59 AM (13 years, 9 months ago)

Because each inoculation point offers another vector for contamination.

It's a matter of probability more than certain failure, but if you have 20 inoculation points you're pretty much asking for trouble. Four is just a number a lot of people seem to like.


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OfflineNeobean
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Re: How much LC should I use per jar? [Re: figgusfiddus]
    #7280615 - 08/10/07 10:12 AM (13 years, 9 months ago)

And besides, if you have a long needle, who needs more than 2-3?


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InvisibleThePyschonaut52
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Re: How much LC should I use per jar? [Re: Neobean]
    #7280616 - 08/10/07 10:14 AM (13 years, 9 months ago)

i use 4 to ensure quick growth yet low chance of contamns


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