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Transfering mycellium ??
    #7269772 -

Well a i have some mycellium growth from some mushroom butts,I took about 5-7 butts and put them into a cardboard container ,When a tin bottom and plastic top.I put the top on added just a little water.It has now been 24 hours and the myc has spread rythmaticly all over the manure/mushroom butts.Is there a way i can possibly trasnfer some of this growth to a jar of grans/corn ?


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There are allot of doors in the world but only one leads out of it

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Re: Transfering mycellium ?? [Re: lovvingcubies]
    #7269819 -

pics??


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if you have any questions please feel free to pm me , thx :-)

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Re: Transfering mycellium ?? [Re: royer]
    #7270044 -

give me 4.7 minutes and pictures you shall have


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There are allot of doors in the world but only one leads out of it

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Re: Transfering mycellium ?? [Re: lovvingcubies]
    #7270078 -

1.are you sure it is not acobweb??(smell like roting fruit)
2.when not...well try it.
3.if you try it put enough mycelium for fast colonisation(1/4 for expl.)

good luck!


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bonjour les shroom!!!

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Re: Transfering mycellium ?? [Re: lovvingcubies]
    #7270082 -






Ineed help fast as im sir contams are about a 99.9% chance unless i can transfer some of this myc growth onto a sterile food source


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There are allot of doors in the world but only one leads out of it

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Re: Transfering mycellium ?? [Re: lovvingcubies]
    #7270120 -

i see it...
i will say, let colonize it full before transfering.
28°c darkness.... uknow...
2 or 3 days


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bonjour les shroom!!!

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Re: Transfering mycellium ?? [Re: jeanpignon]
    #7270140 -

the straw is here the problem for the transfere to grain.
im worry about it.
so....why not colonize pastezrized manure with it??
they are not so much risk of contam then with grain...or??
and its easy.


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bonjour les shroom!!!

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Re: Transfering mycellium ?? [Re: jeanpignon]
    #7270161 -

Ive have tried and cant find a sutibale manure that the myc will take over (other then from the feild) ,maybe some horse manure from a feild? unless you can recomend a brand from the store


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There are allot of doors in the world but only one leads out of it

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Re: Transfering mycellium ?? [Re: jeanpignon]
    #7270168 -

and your chunks are nothing about sterile anymore.
so sterile grain will fast give you the answer of your quest.
let it colonize first.
wenn fully colonized the shroom will produce natural antibiotics
and protect the whole culture.
than transfer to maure


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bonjour les shroom!!!

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Re: Transfering mycellium ?? [Re: jeanpignon]
    #7270183 -

http://www.the-shroom-room.com/store/
Mushroom grow kits, substrate, culture syringes and mycology supplies

try there... it is chip.
but the pitfall is the moisture contain.
you surely make it too wet


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bonjour les shroom!!!

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Re: Transfering mycellium ?? [Re: jeanpignon]
    #7270189 -

please take composted manure.
the fresh one will not work because of the amoniac.
or cook it first....but it is a bit ugly.


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bonjour les shroom!!!

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Re: Transfering mycellium ?? [Re: jeanpignon]
    #7270195 -

no need to be hurry man!!
the shroom will work for you.
go try and go in peace.


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bonjour les shroom!!!

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Re: Transfering mycellium ?? [Re: jeanpignon]
    #7270264 -

try what? sorry i dont understand? I understand the growing process,I myself have never grown other then just taking patties home and making small patchs .Nothing indoor,Just asking how i would trasnfer a small bit of myc to a jar of sterile grain or corn


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There are allot of doors in the world but only one leads out of it

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Re: Transfering mycellium ?? [Re: lovvingcubies]
    #7270302 -

Soooo!
1.waht kind of mush it is??
2.look for they grow parameter.
3.your dirty chunks will spoil the grain before they colonize
so you need a not so nutrient rich and sensytive substrat
4.if you know nothing abut home growing, check first the growing section
you will find a lot of answers.

i can't tell you nothing more so far....sorry!


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bonjour les shroom!!!

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Re: Transfering mycellium ?? [Re: jeanpignon]
    #7270321 -

but if you like... here the camikaze tek.
sterilize grain in jars(grow section)
put your stuff on it wenn cold. so fast and so clean you cann.
incubate at temp good for ur strain(27-30°c for cubies)
after one week check whats happen.
banzai!!


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bonjour les shroom!!!

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Re: Transfering mycellium ?? [Re: jeanpignon]
    #7270331 -

Is it possible i can just take a tissue sample from the myc and transfer it into the grains? rather then just dumping all the nasty manure that has been outside into the grains? its cubes (gulf coast) picking loccaly :smile: .BUT  the down side is that there was also a few copes in the batch so hopefully the cubensis myc will take over the cope myc.


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There are allot of doors in the world but only one leads out of it

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Re: Transfering mycellium ?? [Re: lovvingcubies]
    #7270408 -

first let colonize the stuff in your cup and in darkness by the right
temp.
then transfer the wohle thing not just a sample.
what you want is to colonize so fast as possible to prevent competitor
because they are surely there.
then once that your grain is colonized you can colonise other jars.
DO YOU ANDERSTAND NOW!!


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bonjour les shroom!!!

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Re: Transfering mycellium ?? [Re: jeanpignon]
    #7270461 -

i must warn you!!
there is no garanty to goal succes with this.
but i see this as the best solution to your wish.


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bonjour les shroom!!!

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Re: Transfering mycellium ?? [Re: jeanpignon]
    #7276193 -

The myc is really taking over,Healthy myc. Hopefully cobweb wont take over which seems to happen allot when taking colinized patties home from the field.Ill take a picture to show the progress of it,Id say its pretty much ready.I cant spot any forieng contams,How would i got about mixing it with the grain? just mix it in with a jar of sterile rye grains or birdseed or whatever


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There are allot of doors in the world but only one leads out of it

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Re: Transfering mycellium ?? [Re: lovvingcubies]
    #7276242 -







sorry for the horrible pictuers but you guys get the idea i guess? can anyone else give me anymore information to help this process out a bit


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There are allot of doors in the world but only one leads out of it

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Re: Transfering mycellium ?? [Re: lovvingcubies]
    #7276725 -

ANYONE?


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There are allot of doors in the world but only one leads out of it

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Re: Transfering mycellium ?? [Re: lovvingcubies]
    #7276917 -

Ive tried searching and cant turn up much,Is it impossible to take pure myc and insert it into a sutibiale subtrate creat more heathly myc? I guess its time for experimenting!


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There are allot of doors in the world but only one leads out of it

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Re: Transfering mycellium ?? [Re: lovvingcubies]
    #7276919 -

Sorry for my harsh spelling,Rolf.It was never one of my strong points


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There are allot of doors in the world but only one leads out of it

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Re: Transfering mycellium ?? [Re: lovvingcubies]
    #7276930 -

MAN, your asking have no answer?
why?
maybe because is no good answer to it.
try your shit.
when you realize its fucked...comeback and we talk about it OK??


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bonjour les shroom!!!

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Re: Transfering mycellium ?? [Re: jeanpignon]
    #7277195 -

Sorry im still lost? Do you speak a second laungne? Were just unclear,I dont mean to start trouble or anything but you arent making much since "your asking have no answer?" I just dont understand you. Where are you from? ,But How is it fucked anyways? why cant we talk about it right now? Im really confused dude,If you speak french or somthing speak it and ill user a online translater making it a bit les confusing lol.Peace and Love from florida,

Big bear


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There are allot of doors in the world but only one leads out of it

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Re: Transfering mycellium ?? [Re: lovvingcubies]
    #7277215 -

He is french.

here is a link that may help you:

http://www.shroomery.org/5207/What-is-G2G-and-how-is-it-done
This is not quite what you are trying to do, but you can improvise.


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If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!

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Re: Transferring mycelium ?? [Re: Neobean]
    #7277310 -

OK, sorry!
i just want to tell you that, what you attempt to do,i think,
have no chance of success,but just try and learn from your mistake,
I'm sorry for my bad English i will try to be better,
i Hope we have no stress, i did just try to help you to understand
that they are other safer ways then with grain.
but read the grain preparation of this tek...
there are many picture to understand. http://www.shroomery.org/8432/From-syringe-to-print-using-rye
From syringe to print using rye


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bonjour les shroom!!!

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Re: Transferring mycelium ?? [Re: jeanpignon]
    #7277331 -

C'est drole, tu parles exactement comme mon père quand il parle en anglais... :grin:


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If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!

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Re: Transferring mycelium ?? [Re: Neobean]
    #7277344 -

te fous pas de ma gueule hein!!:blush:
c'est aser dure comme ca.:crazy2:
il fais quel temp au canada?


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bonjour les shroom!!!

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Re: Transferring mycelium ?? [Re: jeanpignon]
    #7277354 -

en plus je suis pas un champion en ortographe,ni en anglais ni en francais. trop longtemps a l'etranger je crois.


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bonjour les shroom!!!

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Re: Transferring mycelium ?? [Re: jeanpignon]
    #7277372 -

Ce sont les avantages et désavantages de l'aventure. Il fait correct ici, sans plus. On a pas eu les grosses chaleurs des années antérieures. c,est parfait pour la pousses à l'intérieur.

Faudra transporter notre conversation ailleurs si on ne veut pas se faire avertir, tu as accèes à ta boite privée?


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If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!

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Re: Transferring mycelium ?? [Re: Neobean]
    #7277382 -

i must look i'm new here....since 3 day or so.


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bonjour les shroom!!!

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Re: Transferring mycelium ?? [Re: jeanpignon]
    #7277389 -

On the top of your page you have :

Home · Pics · Threads · Journal · Messages · [Logout]

clic on messages...


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If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!

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Re: Transferring mycelium ?? [Re: Neobean]
    #7278024 -

Ty very much for the link,Just what i was looking for.


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There are allot of doors in the world but only one leads out of it

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Re: Transferring mycelium ?? [Re: lovvingcubies]
    #7278101 -

lovving cubies hey dude I see what you wanna do but I think putting that in sterile grains would be sure thing for contams...you would probably need agar to turn what you got into growing material.


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I Don't come to fight flesh and blood but spiritual wickedness in high and low places

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Re: Transferring mycelium ?? [Re: VampireSlayer]
    #7278106 -

VampireSlayer said:
lovving cubies hey dude I see what you wanna do but I think putting that in sterile grains would be sure thing for contams...you would probably need agar to turn what you got into growing material.



This dude has got a point....since you can't sterilize whats in that bucket, you would have to put some of that mycelium (and ONLY mycelium) on agar...


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If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!

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Re: Transferring mycelium ?? [Re: Neobean]
    #7280354 -

Thats what i was asking ealier,If i could somehow take a "tissue" sample from the mycellium and just use that.But how? i dont understand agar?


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There are allot of doors in the world but only one leads out of it

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Re: Transferring mycelium ?? [Re: lovvingcubies]
    #7280364 -

Look, there's only one way to clean up a sample from the field, and that's with agar. It sounds like that might be a bit much for your experience level at this point, but it's not a whole lot to learn if you take it slow.

To start: Agar is a sort of gelatinous suspension medium--a lot like gelatin, except you can't use gelatin because the mycelium will eat it up and turn it to mush. You can get it from a health food store, where it's often sold as a vegan alternative to gelatin (which is derived from bone meal).

Agar, mixed with a malt extract or other nutritious substance and sterilized, is poured out into petri dishes, where it solidifies. You then introduce tissue samples to the petri dishes, where they will grow. With a dirty sample (like from the field), contams will grow too. You isolate the good tissue and move it to new agar, where it will grow... maybe with more contams. You repeat until you can grow tissue with no contaminants, then introduce that tissue to grain, preferably in a still-air/glove box (or flowhood, if you have thousands of dollars to spare). This will then colonize the grain happily, and you can then spawn that grain to bulk (like more poo, or whatever you want).

Your head is probably reeling at this point. This is why most people start with spores ordered online. :smile:


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FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS
FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS
FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS

Edited by figgusfiddus (08/10/07 04:32 AM)

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Re: Transferring mycelium ?? [Re: figgusfiddus]
    #7280386 -

figgusfiddus said:
Your head is probably reeling at this point. This is why most people start with spores ordered online. :smile:



Lol Figgus. At this point I think each of us has had his/her head spinning at least once either for ingesting shrooms or trying to get a better yield. :smile: But you,re still right, starting with spores is easy, and allows you to appreciate the whole process better...


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If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!

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Re: Transferring mycelium ?? [Re: Neobean]
    #7280799 -

you can make a glove box for $10 bux or less just get a rubber tote and cut holes for your arms. That will work just fine for agar and g2g...but like figgus said you probably aren't ready for agar at this point. The easiest way for a beginner is getting a spore syringe from a reputable vendor. I started with spore syringes but if I could go back and start over I would start with agar because syringes really teach bad growing technique.


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I Don't come to fight flesh and blood but spiritual wickedness in high and low places

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Re: Transferring mycelium ?? [Re: VampireSlayer]
    #7280806 -

Hey, learning bad growing technique and then learning why it's bad through trial and error is the best learning experience you can have.


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FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS
FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS
FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS

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Re: Transferring mycelium ?? [Re: figgusfiddus]
    #7280824 -

figgusfiddus said:
Hey, learning bad growing technique and then learning why it's bad through trial and error is the best learning experience you can have.



Nothing better than a good screw up to get you back on the right track. :thumbup:


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If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!

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