Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   MagicBag.co All-In-One Bags That Don't Suck   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineneesters
Stranger
Registered: 08/01/07
Posts: 1
Last seen: 15 years, 3 days
Sterilization Question
    #7248119 - 08/01/07 04:00 PM (16 years, 7 months ago)

I'm very new to cultivation, so I have a question about sterilizing the culture jars: Would it be a problem to sterilize the jars and just leave them for a while as I wait for my spore syringe? so i can be prepared when it gets here? or will leaving them for many days likely cause a contamination of some sort?

Extras: Filter Print Post Top
InvisibleThePyschonaut52
Stranger
Male

Registered: 04/24/07
Posts: 982
Re: Sterilization Question [Re: neesters]
    #7248207 - 08/01/07 04:21 PM (16 years, 7 months ago)

actually, leaving them for a few days is recommended to ensure the jars are sterile.


--------------------
"In god we trust..."


-I guess we're screwed.

Extras: Filter Print Post Top
Invisiblemycocurious
Mike O. Kuerias
Male User Gallery


Registered: 02/09/07
Posts: 1,265
Re: Sterilization Question [Re: ThePyschonaut52]
    #7248367 - 08/01/07 05:05 PM (16 years, 7 months ago)

not recommended by me though...


--------------------
:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

- How Myco-Curious Prepares Coir & Compost Substrates
- How Myco-Curious Builds A Bulk Humidifier
- How Myco-Curious Builds An Automated Greenhouse
------------------------------------
figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.

Extras: Filter Print Post Top
OfflineNStoney
Stranger
 User Gallery


Registered: 06/24/07
Posts: 105
Last seen: 16 years, 4 months
Re: Sterilization Question [Re: ThePyschonaut52]
    #7248372 - 08/01/07 05:07 PM (16 years, 7 months ago)

My understanding is that when you PC a jar you either killed everything or you didn't. When you wait a couple days, if no contams pop up then you probably got everything, otherwise you will surely start to see signs of contams.

Spend some time in the contam forum though, some contams are hard to recognize. Bacteria "sour rot" can be a tough one for newb's cuz alot of the time it doesn't have any pronounced color, it will look like a thin coat of slime.

In your situation I would definatly PC 'em before the spores arrive, and then just to be safe i'd re-PC them again before innoculating. The 2nd PCing will surely kill anything that the first one missed. (not just cuz your PCing again, but because anything that slipped past the first PC will start to germinate making it more susceptible to PCing the 2nd time)

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   MagicBag.co All-In-One Bags That Don't Suck   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale


Similar ThreadsPosterViewsRepliesLast post
* A few questions about PCing and JARS... greenlight 1,589 10 06/19/02 07:15 AM
by bassplayer74
* Sterilization Questions Ripple 921 2 08/16/02 04:25 PM
by staindblue
* Sterilization Question Infadel 2,024 9 10/15/01 12:28 PM
by blackout
* Re: Sterilization Question MisterShroom 785 2 03/21/01 02:09 AM
by Kast
* Sterilization question duss69 664 1 12/14/01 12:36 PM
by Dorin
* Needle Sterilization Question RageOholic67 3,122 14 11/08/02 04:19 PM
by RageOholic67
* 1/2 pint jars steam sterilize question FongSaiYuk 2,091 4 10/01/02 12:45 PM
by mickey_rourke
* Quick sterilization question? The_Mad_Russian 808 1 02/05/02 11:36 AM
by arkendave

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
389 topic views. 14 members, 144 guests and 96 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.01 seconds on 14 queries.