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 legallyhomeless
mooch
Registered: 07/01/05
Posts: 4,051
Loc: EZRA for the Refuge
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 Re: LC always contaming my jars  [Re: aspore]
#7235115 - 07/28/07 11:57 PM (14 years, 2 months ago) |
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The spores I used are proven good  And I ALWAYS incubate my jars 1 week before innocing because I dont want to waste spores on a jar that might contam anyhow. I always use bottled water too. I checked earlier and I did use spring water not distilled. Im using regular metal quart lids screwed on hella tight with 2 air holes in the top and 1 small innocing hole (although I just innoc in w/e one I find first) Covered in tyvek and micropore over holes.
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Brainiac
Rogue Scientist
Registered: 04/29/06
Posts: 13,259
Loc: 與您的女朋 
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Do you sanitize the Syringe thats used to incarnate the jars with the lc ?
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 Fair is Fair 
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ThePyschonaut52
Stranger
Registered: 04/24/07
Posts: 982
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Re: LC always contaming my jars [Re: Brainiac]
#7235802 - 07/29/07 09:18 AM (14 years, 2 months ago) |
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make self healing injection ports.
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"In god we trust..."
-I guess we're screwed.
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legallyhomeless
mooch
Registered: 07/01/05
Posts: 4,051
Loc: EZRA for the Refuge
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Of course I sterilize. What does a self healing injection port have to do with anything? Even if I did have one I will still have contams. I dont think its due to not having an injection port
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 beags
don't forget toPanic
Registered: 07/19/07
Posts: 11
Last seen: 10 years, 3 months
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Re: LC always contaming my jars [Re: RogerRabbit]
#7236494 - 07/29/07 03:29 PM (14 years, 2 months ago) |
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Quote:
RogerRabbit said:
Use agar to verify your spores, not LC. LC should not be made except with a proved, isolated fruiting strain. You'll rarely see contaminants in LC because molds generally won't sporulate in water.
RR
would this be like an LC from tissue?
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legallyhomeless
mooch
Registered: 07/01/05
Posts: 4,051
Loc: EZRA for the Refuge
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Re: LC always contaming my jars [Re: beags]
#7236695 - 07/29/07 04:43 PM (14 years, 2 months ago) |
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No and Ive never dont an agar. BUT Im almost positive most people dont and they dont have any problems.
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