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Invisiblelegallyhomeless
mooch
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Registered: 07/02/05
Posts: 4,051
Loc: EZRA for the Refuge
Re: LC always contaming my jars [Re: aspore]
    #7235115 - 07/29/07 06:57 AM (15 years, 4 months ago)

The spores I used are proven good And I ALWAYS incubate my jars 1 week before innocing because I dont want to waste spores on a jar that might contam anyhow. I always use bottled water too. I checked earlier and I did use spring water not distilled. Im using regular metal quart lids screwed on hella tight with 2 air holes in the top and 1 small innocing hole (although I just innoc in w/e one I find first) Covered in tyvek and micropore over holes.


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InvisibleBrainiac
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Registered: 04/30/06
Posts: 13,259
Loc: 與您的女朋 Flag
Re: LC always contaming my jars [Re: legallyhomeless]
    #7235165 - 07/29/07 07:14 AM (15 years, 4 months ago)

Do you sanitize the Syringe thats used to incarnate the jars with the lc ?


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:cool: Fair is Fair :devil:


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InvisibleThePyschonaut52
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Registered: 04/24/07
Posts: 982
Re: LC always contaming my jars [Re: Brainiac]
    #7235802 - 07/29/07 04:18 PM (15 years, 4 months ago)

make self healing injection ports.


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"In god we trust..."


-I guess we're screwed.


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Invisiblelegallyhomeless
mooch
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Registered: 07/02/05
Posts: 4,051
Loc: EZRA for the Refuge
Re: LC always contaming my jars [Re: ThePyschonaut52]
    #7236288 - 07/29/07 08:57 PM (15 years, 4 months ago)

Of course I sterilize. What does a self healing injection port have to do with anything? Even if I did have one I will still have contams. I dont think its due to not having an injection port


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Offlinebeags
don't forget toPanic
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Registered: 07/19/07
Posts: 11
Last seen: 11 years, 4 months
Re: LC always contaming my jars [Re: RogerRabbit]
    #7236494 - 07/29/07 10:29 PM (15 years, 4 months ago)

Quote:

RogerRabbit said:
Use agar to verify your spores, not LC. LC should not be made except with a proved, isolated fruiting strain. You'll rarely see contaminants in LC because molds generally won't sporulate in water.
RR




would this be like an LC from tissue?


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Invisiblelegallyhomeless
mooch
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Registered: 07/02/05
Posts: 4,051
Loc: EZRA for the Refuge
Re: LC always contaming my jars [Re: beags]
    #7236695 - 07/29/07 11:43 PM (15 years, 4 months ago)

No and Ive never dont an agar. BUT Im almost positive most people dont and they dont have any problems.


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