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OfflineTalimor
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Everyones Colonization Times
    #7214400 - 07/23/07 03:52 PM (17 years, 5 months ago)

I've got 12 jars colonizing in the following conditions:

Jar Size: Pint
Substrate: Vermiculite + Brown Rice Flour + water
Inoculated with: B+ Spores
Inoculation Points: 2
Temperature: 78-79F
Light/Dark: Light, but not direct.
Storage: 6 jars each in 2 plastic bins, with lids.

Other: Aluminum foil left on the lids as to not expose the inoculation holes... plus i didn't feel like taking them off.

It's currently day 13 of the colonization process. I have no contaminants as of yet. The mycelium is growing strong, a good 3-5 inch circumference at each inoculation point. When I started I put one 6 jar batch next to my computer (slightly warmer) and the second 6 jar batch in normal room temperature. After about 1.5 weeks I noticed that the first batch next to my computer was doing much better. I removed the second batch from room temperature and placed it with the first batch.

I was hoping to hear other peoples colonization set ups down to the detail, so that I could increase the growth rate and efficiency of my next grow.

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Offlinefiggusfiddus
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Re: Everyones Colonization Times [Re: Talimor]
    #7214419 - 07/23/07 03:56 PM (17 years, 5 months ago)

Temperature is definitely the biggest factor in determining colonization time, followed by genetics.

Twelve jars left in an air-conditioned (~67-71F, COLD) room showed no growth five days after an LC inoculation. Set in the other room, in the cabinet above the fridge (~78-82F), they showed growth after only one more day, and are now nearly colonized four days later.


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Edited by figgusfiddus (07/23/07 04:07 PM)

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InvisibleBridgeburner
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Re: Everyones Colonization Times [Re: figgusfiddus]
    #7214431 - 07/23/07 03:57 PM (17 years, 5 months ago)

my friend just puts the jars into various dark nooks & crannies in her room and lets them slowly colonize. most of them are colonized in a month or two.


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OfflineCaptainLinger
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Registered: 05/25/07
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Re: Everyones Colonization Times [Re: Talimor]
    #7214440 - 07/23/07 04:00 PM (17 years, 5 months ago)

Yeah, keeping that temp range is about all you need to do. Lots of sterile technique, not so much work, with incubation.

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Offlinefiggusfiddus
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Re: Everyones Colonization Times [Re: Bridgeburner]
    #7214468 - 07/23/07 04:06 PM (17 years, 5 months ago)

Quote:

b0red5tiff said:
my friend just puts the jars into various dark nooks & crannies in her room and lets them slowly colonize. most of them are colonized in a month or two.




Woah, a month or two? Someone's keeping them way too cold, way too hot, or is preparing the substrate wrong!

I had some multispore (non-LC) jars take four weeks once, but that was my first attempt and I was still incubating at like 85F back then. Even spores don't take more than three weeks for me now, and LCs often take less than two.


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Offlinesproket13
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Re: Everyones Colonization Times [Re: figgusfiddus]
    #7214504 - 07/23/07 04:18 PM (17 years, 5 months ago)

my jars are on day 7 of 78-84 depending on the time of day. and they are about 50-75% colonized. via spore prints. seems to be going great!

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Offlineaspore
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Registered: 07/10/07
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Re: Everyones Colonization Times [Re: sproket13]
    #7214547 - 07/23/07 04:28 PM (17 years, 5 months ago)

rye with a bit of coir

liquid culture

72-80 depending on time of day

complete dark

penis envy uncut hybrid - 8 days
golden teacher - 9 days
burma - 7 days
chitwan - 10 days

I also have used wheat with slower times


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c        l        i      c        k        m        e

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InvisibleBridgeburner
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Re: Everyones Colonization Times [Re: sproket13]
    #7214551 - 07/23/07 04:30 PM (17 years, 5 months ago)

they're going at 23C, substrate is brf/verm mix, the basic pf-tek formula. for a 250ml jar she uses 140ml verm, adds water and mixes in 40ml of brf.


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OfflineTactile Tunafish
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Re: Everyones Colonization Times [Re: Talimor]
    #7214720 - 07/23/07 05:12 PM (17 years, 5 months ago)
Log in to view attachment

Jar Size: Pint
Substrate: Vermiculite + Brown Rice Flour + water
Inoculated with: TC
Inoculation Points: 4
Temperature: (2) 74F (1) 82F
Light/Dark: Dark (but peeked in on them every 2 days or so due to enormous excitement!
Storage: 3 jars each in kitchen cabinet with metal lids.


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----
TTF
93
and my head is fifty feet high -let my body and soul be my guide

<david byrne from "Glass Concrete and Stone" on Grown Backwards>
93/93

Edited by Tactile Tunafish (07/23/07 05:27 PM)

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Offlinefiggusfiddus
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Re: Everyones Colonization Times [Re: Bridgeburner]
    #7214920 - 07/23/07 06:05 PM (17 years, 5 months ago)

Quote:

b0red5tiff said:
they're going at 23C, substrate is brf/verm mix, the basic pf-tek formula. for a 250ml jar she uses 140ml verm, adds water and mixes in 40ml of brf.




My recipe (and a common one now, though PF used to recommend otherwise) is 2:1:2 (verm:brf:water) by volume. Maybe you'll have more luck with this richer recipe--you should get more yield from it, too, since it has more BRF. Experiment, though, by all means.


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Invisiblejeetered
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Re: Everyones Colonization Times [Re: Talimor]
    #7215541 - 07/23/07 08:34 PM (17 years, 5 months ago)

you're looking at a month to colonize a pftek style pint, i can produce 5lbs dry in that time...rye grain, 5 days, full colonization, via LC, spawn 2 pints per lb of bulk sub, five days colonization, then 6 days to pin, 3 days to mature... wallah.

my question is..... why wait for a pint of brf...

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InvisibleBridgeburner
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Re: Everyones Colonization Times [Re: jeetered]
    #7217196 - 07/24/07 08:56 AM (17 years, 5 months ago)

because i don't need that much mushroom. i like the rule "don't grow more than you can eat". i like cakes because it takes time and i get an exact dose from them. in the end it doesn't matter.


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OfflineCaptainLinger
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Registered: 05/25/07
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Re: Everyones Colonization Times [Re: Talimor]
    #7217202 - 07/24/07 08:58 AM (17 years, 5 months ago)

Quote:

Even spores don't take more than three weeks for me now, and LCs often take less than two.





Yeah, I believe my tubs this morning were created with spawn inoculated on 7/7 with LC.

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Invisiblejeetered
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Re: Everyones Colonization Times [Re: Bridgeburner]
    #7217261 - 07/24/07 09:31 AM (17 years, 5 months ago)

Quote:

b0red5tiff said:
because i don't need that much mushroom. i like the rule "don't grow more than you can eat". i like cakes because it takes time and i get an exact dose from them. in the end it doesn't matter.




good enough answer for me!

with an LC i can get a cake to be fully colonized on the outside in a week, but i'll birth it a week after that, so 2 weeks basically. Letting it sit for a week really seems to help it out for me. (before fruiting it).

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InvisibleBridgeburner
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Re: Everyones Colonization Times [Re: jeetered]
    #7217334 - 07/24/07 09:52 AM (17 years, 5 months ago)

Quote:

jeetered said:
Quote:

b0red5tiff said:
because i don't need that much mushroom. i like the rule "don't grow more than you can eat". i like cakes because it takes time and i get an exact dose from them. in the end it doesn't matter.




good enough answer for me!

with an LC i can get a cake to be fully colonized on the outside in a week, but i'll birth it a week after that, so 2 weeks basically. Letting it sit for a week really seems to help it out for me. (before fruiting it).




your point of view intrigues me :cool:
going to test LC inoculation out some day when fed up with cakes.


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OfflineTactile Tunafish
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Registered: 05/28/07
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Re: Everyones Colonization Times [Re: jeetered]
    #7217475 - 07/24/07 10:43 AM (17 years, 5 months ago)

alas waiting for a pint - the reason in our case is that we haven't been able to get a pressure cooker yet.

Registered with freecycle.org -- putting it out into the universe one magically shows up - I'm just chomping at the bit to switch over to rye/wbs. <<the hpoo is all ready to go.>>


--------------------
----
TTF
93
and my head is fifty feet high -let my body and soul be my guide

<david byrne from "Glass Concrete and Stone" on Grown Backwards>
93/93

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