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Invisiblezanny787
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Correct me if I'm wrong....
    #7214518 - 07/23/07 04:21 PM (17 years, 5 months ago)

okay so is the only differnce between LC and a clone that when you do LC you are using a syringe that you either bought or prepared and when you do a clone, you are using mushrooms that you grew yourself??


Little confused...some help please?

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Offlinefiggusfiddus
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Re: Correct me if I'm wrong.... [Re: zanny787]
    #7215078 - 07/23/07 06:44 PM (17 years, 5 months ago)

Ehhh, kind of. Let's start with the basics:

A spore syringe contains lots and lots of spores. Spores are a bit like sperm, let's say--they can't really turn into a successful organism on their own, and they're all genetically unique (which is why two human fraternal brothers aren't identical twins). However, the difference here is that even though these spores probably came from the same organism originally, they can mate with each other. There's no egg--just spores mating with spores.

However, each syringe is full of thousands upon thousands (if not millions) of spores. Probably only a few dozen or hundred will match up into successful, healthy pairs, but that's still a lot of genetically unique organisms.

So when you shoot spore solution (a spore syringe) into a jar of BRF or whatever substrate, you're actually getting a whole colony of little brothers and sisters, so to speak, competing for space. They all colonize the jar, and some of them steamroller over the others, because some are just naturally way better growers. When you fruit your BRF cake, there may be any number of related, but genetically different colonies on your cake. Any given mushroom, however, can only come from one of these colonies.

When you inject spore solution to make an LC, the same thing happens. You've got hundreds of unique organisms in your LC jar. In turn, if you were to shoot this LC into a prepared BRF jar, you'd be doing the same thing as if you just shot the spores in. People use multispore LC sometimes instead of spore solution because it takes off and colonizes more quickly, plus an LC can let you grow a whole lot of inoculant from a tiny bit of spore solution.

Now, a clone is when a piece of tissue is taken from a single organism--usually from a fruit body (mushroom), since that's the only way to guarantee you've got a tissue sample that's only one organism, genetically. People clone the biggest and healthiest mushrooms in the hope that maybe they're isolating a genetic sample that's going to grow bigger, healthier shrooms in the future.

You can take this tissue sample and you can put it in an LC, or you can put it on a petri dish prepared with agar, or you can even drop it into prepared substrate (usually grain spawn). This is cloned tissue.

So sometimes an LC is full of material all of one genetically unique organism--cloned, so to speak--and sometimes it's multispore, or full of lots and lots of them. Does that make sense?


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Offlinexaxphaanes
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Re: Correct me if I'm wrong.... [Re: figgusfiddus]
    #7215108 - 07/23/07 06:50 PM (17 years, 5 months ago)

Cloning is a weird business because even if you do clone you can still get multiple genetic traits within that tissue sample you took.For example take a tissue sample put it on agar and you can have multiple sectoring.


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Invisiblezanny787
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Re: Correct me if I'm wrong.... [Re: xaxphaanes]
    #7215164 - 07/23/07 07:02 PM (17 years, 5 months ago)

figgus...great answer.

Thank you :smile:

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Offlinefiggusfiddus
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Re: Correct me if I'm wrong.... [Re: xaxphaanes]
    #7215200 - 07/23/07 07:09 PM (17 years, 5 months ago)

Quote:

xaxphaanes said:
Cloning is a weird business because even if you do clone you can still get multiple genetic traits within that tissue sample you took.For example take a tissue sample put it on agar and you can have multiple sectoring.




Tissue from a fruit?

I've never heard of that.


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Offlinexaxphaanes
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Re: Correct me if I'm wrong.... [Re: figgusfiddus]
    #7215208 - 07/23/07 07:11 PM (17 years, 5 months ago)

yea that is correct.


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Offlineraudi1
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Re: Correct me if I'm wrong.... [Re: xaxphaanes]
    #7215494 - 07/23/07 08:20 PM (17 years, 5 months ago)

Groups of cells make up tissue, groups of tissue make an organ/fruit.

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