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Offlinecoconutjamushies
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Registered: 11/18/04
Posts: 180
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New spore syringe tek
    #7182711 - 07/16/07 10:25 AM (17 years, 6 months ago)

I made a fresh spore print about a few hours ago, then after removing the cap I immediately made sucked the spores from the print.
What do you guys think about it?
The syringe was so full of spores that it was almost black:grin:


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OfflineMephisto616
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Re: New spore syringe tek [Re: coconutjamushies]
    #7182719 - 07/16/07 10:26 AM (17 years, 6 months ago)

Whats the tek? pics would be nice


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OfflineNeobean
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Re: New spore syringe tek [Re: Mephisto616]
    #7182738 - 07/16/07 10:39 AM (17 years, 6 months ago)

Quote:

Mephisto616 said:
Whats the tek? pics would be nice




I agree...pIcs and Details would be nice!!!


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OfflineMYSTIQUE
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Re: New spore syringe tek [Re: Neobean]
    #7182754 - 07/16/07 10:45 AM (17 years, 6 months ago)

Thats not a Tek. Its not even a sentence.
"then after removing the cap I immediately made sucked the spores from the print."

you made sucked?

Less weed and read threw it at least once after please :laugh:


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Offlineoutlawimmortal
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Re: New spore syringe tek [Re: coconutjamushies]
    #7182767 - 07/16/07 10:49 AM (17 years, 6 months ago)

i think the original poster means that he just had a sterilised syringe (nothing in it), removed the cap that protects the needle, then sucked the spores from the spore print into it, then sucked up water.

seems a nice idea


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InvisibleGoodbyeOrb
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Re: New spore syringe tek [Re: coconutjamushies]
    #7182812 - 07/16/07 11:00 AM (17 years, 6 months ago)

was the water sterile? I'm betting it's contaminated.

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Offlinecoconutjamushies
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Registered: 11/18/04
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Re: New spore syringe tek [Re: GoodbyeOrb]
    #7184839 - 07/16/07 08:01 PM (17 years, 6 months ago)

Guys, sorry for not writing it down clearly. I was sort of blazed last night haha
It's like this
1. Make fresh spore print
2. Remove mush cap from *fresh print (the print is not yet dry)
3. Immediately squirt water onto the print
4. Suck up the spores

* The syring I made was so dark from all the spores. When I say dark, I mean, DARK
* imho, the spore syringe will be fresher and be more hydrated.
I hope I've helped:grin:
* I'll update this soon


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InvisibleBlutjager
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Re: New spore syringe tek [Re: GoodbyeOrb]
    #7184858 - 07/16/07 08:05 PM (17 years, 6 months ago)

Quote:

GoodbyeOrb said:
was the water sterile?  I'm betting it's contaminated.




That was the 1st thing I thought as well:(

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OfflineRugbyhooker
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Re: New spore syringe tek [Re: Blutjager]
    #7184909 - 07/16/07 08:14 PM (17 years, 6 months ago)

A good way to do that is to make the print in a small jar or tupperware container covered with tinfoil loosely. Then you squirt in a syringe full of sterile water and it's black.


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Offlinecoconutjamushies
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Re: New spore syringe tek [Re: Rugbyhooker]
    #7187349 - 07/17/07 09:39 AM (17 years, 6 months ago)

Why do you guys say the water is contaminated? I was using sterile water and syringes.
Guys, I just need some of your feedback on what I did:grin:


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Invisiblesimplemachine
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Re: New spore syringe tek [Re: coconutjamushies]
    #7187395 - 07/17/07 09:56 AM (17 years, 6 months ago)

Going back to your first post I can't even tell what you're talking about!

It is difficult for me to give my feedback on a procedure that was not explained in the first place.


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OfflineSuperFunGuy
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Re: New spore syringe tek [Re: coconutjamushies]
    #7187399 - 07/17/07 09:59 AM (17 years, 6 months ago)

I agree. You should definitely rewrite it..


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InvisibleLayYouIn
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Re: New spore syringe tek [Re: coconutjamushies]
    #7188080 - 07/17/07 01:08 PM (17 years, 6 months ago)

Quote:

coconutjamushies said:
1. Make fresh spore print
2. Remove mush cap from *fresh print (the print is not yet dry)
3. Immediately squirt water onto the print
4. Suck up the spores





this is what i do but i use clorox bleach wipes in the process.  i've tried one syringe out and it worked fine.  they are dark because i usually only make a few at a time.  im going to try the others out soon.  you should add this into your tek and take some pictures...:)

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OfflineLVL12
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Re: New spore syringe tek [Re: LayYouIn]
    #7188127 - 07/17/07 01:17 PM (17 years, 6 months ago)

Seems like it should work, w/ a slight increase risk of contamination. But I wouldnt really call it a "new spore syringe tek"

(i personally am in love with the ziplock baggy print/syringe tek)


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Disclaimer: Posts from LVL12 are strictly for entertainment and are a work of fiction, nothing written or said by LVL12 should in interpreted as truth, fact or by their literal definitions or translations. Anyone who reads/listens to these words does so at their own risk, parental discretion is advised.

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OfflineDem_Bones
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Re: New spore syringe tek [Re: LVL12]
    #7188585 - 07/17/07 03:02 PM (17 years, 6 months ago)

the only way you could call this new or even a tek is if you, did almost no research on the hundreds of post and methods outlined here.

and then there is the whole augment made by all most all the old timers that, a think black syringe is not better but worse
and to use a whole print for one is just dumb
,,look here at the life cycle of mushrooms.

The Life Cycle of a Mushroom
An interesting image detailing a mushroom's journey from spores into adulthood.

Haploid basidiospores germinate in a suitable environment and grow into short-lived haploid mycelia.

Undifferentiated hyphae from two haploid mycelia of opposite mating type undergo plasmogamy,
creating a dikaryotic mycelium that grows faster than, and ultimately crowds out, the parent haploid mycelium. The mycelium of the mushroom illustrated here (Cortinarius) forms mycorrhizae with trees.

Environmental cues such as rain, temperature changes, and, for mycorrhizal species, seasonal changes in the plant host,
induce the dikaryotic mycelium to form compact masses that develop into mushrooms.

Cytoplasm streaming in from the mycelium and from the attached mycorrhizae swells the hyphae of mushrooms, causing them to "pop up" overnight. The dikaryons of basidiomycetes are long-lived, generally producing a new crop of basiocarps (mushrooms, in this case) each year.

Karyogamy occurs in the terminal dikaryotic cells that line the surfaces of the gills (SEM at left).

Each cell swells to form a diploid basidium, which rapidly undergoes meiosis and yields four haploid nuclei.

The basidium then grows four appendages, and one haploid nucleus enters each appendage and develops into a basidiospore.
When mature, the basidiospores are propelled slightly (by electrostatic forces) into the spaces between the gills. After the spores drop below the cap.they are dispersed by the wind.



So you see here after you have a susesfull mateing and have dikaryotic mycelium , it croweds out all the unpaired mono mycelium
but you will have several successful dikaryotic pairs that are incompatible with each other and will compete with each other in your grain spawn and trays cakes , whatever. so more is not better.

this is why all commercial grower and old hands make a culture on a petry dish and select the most vigorous and healthy dikaryotic mycelium set, and cut that section out and move it off to be used for growing. that way there is just one set of dikaryotic mycelium and it will act the same every time -environment.
a side note, large mats of spores can keep each other from germinating
i have seen it in petri dishes where a large flake fell in to the agar and there where just to many in one some spot , it don't know if its a chemical thing or thy just choke each other out, but Paul talks about it in the MC

Edited by Dem_Bones (07/17/07 03:07 PM)

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InvisibleGoodbyeOrb
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Re: New spore syringe tek [Re: LVL12]
    #7188601 - 07/17/07 03:07 PM (17 years, 6 months ago)

Quote:

LVL12 said:
(i personally am in love with the ziplock baggy print/syringe tek)




Do you have a link? Can't find anything labeled accordingly.

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OfflineLVL12
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Re: New spore syringe tek [Re: GoodbyeOrb]
    #7188739 - 07/17/07 03:46 PM (17 years, 6 months ago)

I guess its called the "lazy bastard's syringe tek" or something of that nature, its been awhile since i've read the tek, I didn't even realise some modifications i have made to it, (for instance instead of rubbing alcohol to clean the bag, i use a lysol wipe, its just alot easier to manipulate and easy to spot the residue (it looks soapy)

http://www.shroomery.org/9210/MultiSyncs-lazy-bastard-print-syringe-guide


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Disclaimer: Posts from LVL12 are strictly for entertainment and are a work of fiction, nothing written or said by LVL12 should in interpreted as truth, fact or by their literal definitions or translations. Anyone who reads/listens to these words does so at their own risk, parental discretion is advised.

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Offlinedeformedreality
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Re: New spore syringe tek [Re: coconutjamushies]
    #7188751 - 07/17/07 03:48 PM (17 years, 6 months ago)

*stabs thread with a big knife 2000 times*


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OfflineAbyssWP
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Re: New spore syringe tek [Re: LVL12]
    #7188756 - 07/17/07 03:51 PM (17 years, 6 months ago)

yea this in no way is a new tek.. anyways lvl12 could you give me details on how you do that tek, i was thnking of doing that tek for my first print but wont there be contams on the cap and everything, or do you just follow it exactly? thanks

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OfflineLVL12
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Re: New spore syringe tek [Re: AbyssWP]
    #7188792 - 07/17/07 04:01 PM (17 years, 6 months ago)

Quote:

AbyssWP said:
yea this in no way is a new tek.. anyways lvl12 could you give me details on how you do that tek, i was thnking of doing that tek for my first print but wont there be contams on the cap and everything, or do you just follow it exactly? thanks




I worried about that at first too, but so far, having done that tek approx 40 times, have not experienced any contaminated syringes. if you're super worried you can always clean the caps I read on here somewhere how to do it, i believe it was either related to cloaning or agar. I've actually gotten more lazy and a little more sloppy with it, and still haven't run into a problem (YET) as soon as i do i'll refine that aspect of it a bit as well, i'm constantly tweaking all parts of the growing process to suite me a little better. For instance I once fried two caps by not soaking up all the alcohol in the bag, thats when i switched to using the lysol wipes. Also I've poked my syringe straight through the bag many times and either sprayed myself with the water or had the spore water leak all over me. From here I decided to put some medical tape on two sides of the bag in order to have an area to poke through and sort of a barrier to assist in keeping the syringe from going through the bag.


--------------------
Disclaimer: Posts from LVL12 are strictly for entertainment and are a work of fiction, nothing written or said by LVL12 should in interpreted as truth, fact or by their literal definitions or translations. Anyone who reads/listens to these words does so at their own risk, parental discretion is advised.

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