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this tamp culture jo has does not seem to want to produce fruits which will open their caps. Fine, so he's going to take them. The cap begins to separate from the stem, but they don't open more than a few mm's before pooping out. What he did was take one of the mushrooms with a cap that was open a bit, and cut it open. When he did this, he noticed that the gills appeared brownish. We are hoping this is indicative of the presence of spores. This done, he discarded the cap (it is certainly very contaminated from all of the handling). He took another cap at the same point in development, and put it in a semi-sterile environment to let it dry for a couple days. The next step he will do, is put it into a sterile halfpint and shoot some sterile H2O at it a few times with a 10cc syringe, then suck it up. If any mycelium begins to grow on the plates that he innocs, I think he can he assume that the new growth is from the spores (since the cap was dried) and not the original culture.
I thought of this out of frustration, as well as remembering that Workman did something similar to get the A and B mexicana strains. Any ideas here? Any thoughts on modifications jo should make?
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