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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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Invisiblecontaman
journeyman
Registered: 05/27/02
Posts: 53
working with what these tamps are giving...
    #718606 - 07/02/02 06:01 PM (21 years, 5 months ago)

So,

this tamp culture jo has does not seem to want to produce fruits which will open their caps. Fine, so he's going to take them. The cap begins to separate from the stem, but they don't open more than a few mm's before pooping out. What he did was take one of the mushrooms with a cap that was open a bit, and cut it open. When he did this, he noticed that the gills appeared brownish. We are hoping this is indicative of the presence of spores. This done, he discarded the cap (it is certainly very contaminated from all of the handling). He took another cap at the same point in development, and put it in a semi-sterile environment to let it dry for a couple days. The next step he will do, is put it into a sterile halfpint and shoot some sterile H2O at it a few times with a 10cc syringe, then suck it up. If any mycelium begins to grow on the plates that he innocs, I think he can he assume that the new growth is from the spores (since the cap was dried) and not the original culture.

I thought of this out of frustration, as well as remembering that Workman did something similar to get the A and B mexicana strains. Any ideas here? Any thoughts on modifications jo should make?


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OfflineWorkmanV
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Re: working with what these tamps are giving... [Re: contaman]
    #720230 - 07/03/02 11:21 AM (21 years, 5 months ago)

That sounds reasonable. Or you can gently crush the dried cap and streak the dust. The only problem I can think of is you aren't likely to see any improvement in the strain after doing this.


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Invisiblecontaman
journeyman
Registered: 05/27/02
Posts: 53
Re: working with what these tamps are giving... [Re: Workman]
    #721946 - 07/04/02 05:31 AM (21 years, 5 months ago)

Thanks Workman,

I think your method sounds easier with less opportunity for contaminants to enter. Either way, I expect that jo will need to isolate any mycelium from contams.

I guess I was just hoping that beginning from spores again, would bring back some vigor, but my bubbles keep getting burst... lol

The iso seems to do wonderfully on agar, and sclerotia form readily on the agar, and in the casing, but the fruits just don't mature nicely... aarrgghh..

I guess jo should just buy a new print to work with...


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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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