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Bloodhound
Sleeping on thejob



Registered: 10/12/05
Posts: 103
Last seen: 3 years, 9 months
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trich spores in syringe?
#7182410 - 07/16/07 07:49 AM (16 years, 7 months ago) |
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I have some suspicion that one of my syringes has trich mixed with the spores.
I knocked up 4 jars for cambos, 4 for GT and kept one WBS jar untouched to use as a test jar. After 5 days, I find that GT is colonizing all 4 like wildfire, test jar is clean as the day it came out of the PC, but Cambo jars are contaminated with trich.
I've also made LCs with both of the syringes like 3 days ago, I find that cambos is already filled with brownish specks after a day, while tiny white fluffy balls are just showing up in GT now. There were no precipitate when the LCs came out of the PC but now in Cambo LC, there's a lot of them. I've compared this to another member's contam'd jars: http://www.shroomery.org/forums/showflat.php/Number/7030311#Post7030311 and ours are pretty similar except mine is darker in color.
Here's a pic of my Cambo LC:

Note: I don't think inoculation introduced contams since I did it in the cleanest room of the house with still air (which I sprayed with oust) and wiped down all surfaces with lysol and left it for about 10 minutes while I took a shower, wore a longsleeves which had been sprayed with lsyol as well, cleaned my hands in bleachwater, used alcohol soaked paper to hold the needles, and flame sterilized it before injecting it into the jar. I kept the process as sterlized as possible.
GT is doing great and the test jar is clean. So to me, it seems pretty likely that trich spores were in the cambo syringe from the beginning.
Am I right in thinking that?
If so, How do I ask the vendor (who shall remain unnamed because I haven't spoke to them yet and I want to confirm this first with you guys) to replace the syringe? I can't as well tell them how I know it's contaminated since it's illegal to cultivate psilocybe mushrooms and I don't own a microscope.
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figgusfiddus
Arrogant Worm


Registered: 02/02/07
Posts: 2,126
Loc: Figgus, Fiddia
Last seen: 15 years, 4 months
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Re: trich spores in syringe? [Re: Bloodhound]
#7182459 - 07/16/07 08:14 AM (16 years, 7 months ago) |
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If four-of-four GTs work out, and four-of-four cambo jars go bad with the same condition, you've got bad inoculant. Not sure why you're so tentative to think so. 
Also, you may notice that if you look really hard, you DO have a microscope. Lucky you! Ooh, it's a nice one too. Now tell the vendor you've seen unusual ascomycete spores all throughout your spore solution. What a shame! I sure do hope they replace it. Etc., etc.
Vendors will know what you mean. They're not dummies, and I'm sure they don't really think all their cash is coming from folks who have an unusual interest in observing ungerminated cubensis spores. If they're a Shroomery sponsor (i.e. verifiably legit vendor), they're sure to replace your syringe.
Good luck with the other grows!
Edit: Oh uh, did you use all of your spores from that syringe? If not, try shooting them straight into another jar, or some agar. It might be that your LC is contaminated but not the syringe.
-------------------- FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS
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Bloodhound
Sleeping on thejob



Registered: 10/12/05
Posts: 103
Last seen: 3 years, 9 months
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Re: trich spores in syringe? [Re: figgusfiddus]
#7182480 - 07/16/07 08:24 AM (16 years, 7 months ago) |
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Oh darnit, there *is* my microscope, hadn't seen it in all those years.
I did the jars by multispore inoculation, the LCs came a few days after that. SO it is my syringe after all.
I just needed the technical wording of a mycologist to make it look legit. :P
Thanks man.
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