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 psiloglow
Stranger
Registered: 07/07/07
Posts: 115
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 Misting the Casing? How often H2O2 OK in the mix? 
#7182366 - 07/16/07 07:26 AM (15 years, 10 months ago) |
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I was going to boil some filtered or distilled water, then store in fridge for misting. I was wondering if it is safe to put a little H2O2 into the water and directly mist the casing. And if so, how often? Also I wanted to add some anti-biotic to the mix, like Tetracycline or Doxycycline, which should inhibit bacterial growth. Anyone ever do either of these and is it safe? Thanks.
Psiloglow--
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 MYSTIQUE
Say Hi to the elves for me.
Registered: 04/28/07
Posts: 1,764
Loc: Canada
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: psiloglow]
#7182628 - 07/16/07 09:43 AM (15 years, 10 months ago) |
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Some just use tap water not boiled or anything and it works fine so to boil distilled water will probably be a a waste.
H202 will slow down growth and do more damage then good. It is only used in trying to save them from cobweb and not as precaution.
tetracycline ? do your shrooms have chronic prostatitis, sinusitis, syphilis, chlamydia, pelvic inflammatory disease,Elephantiasis, acne and rosacea or anthrax? If not I would leave out the tetracycline and doxycline. But if you do give them the doxycycline don't forget Doxycycline impairs the effectiveness of many types of contraceptive pill due to CYP450 induction So look out and use extra protection or you will end up a daddy
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Dont know what the fuck I just said? READ THIS
http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary
I ain't a hippy but I'm covered in dirt
Sippin lots of mushroom tea in a tye-dye shirt
Chasin' the Grateful Dead, no shoes on my feet
Beggin' in the parking lot for something to eat,
 DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!
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shagg
nottanub
Registered: 02/07/07
Posts: 448
Loc: South
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: MYSTIQUE]
#7182633 - 07/16/07 09:44 AM (15 years, 10 months ago) |
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h202 is unnecessary
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psiloglow
Stranger
Registered: 07/07/07
Posts: 115
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: shagg]
#7182672 - 07/16/07 10:00 AM (15 years, 10 months ago) |
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Thanks for the Bio-chem lesson. I'm a pharmD by the way. Cyp450? What about the AUC and t1/2 lol? ....LOL..many bacterial infections may be prevented by using tet in the perlite and casing. Many. And no my girl does not take tet with BC together..lol. I'm well aware. But please amuse me with your pharmacodynamic lessons anytime.
Psiloglow
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MYSTIQUE
Say Hi to the elves for me.
Registered: 04/28/07
Posts: 1,764
Loc: Canada
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: psiloglow]
#7182684 - 07/16/07 10:06 AM (15 years, 10 months ago) |
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If ya already think ya know the answer why even ask Mr.Doctor?
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Dont know what the fuck I just said? READ THIS
http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary
I ain't a hippy but I'm covered in dirt
Sippin lots of mushroom tea in a tye-dye shirt
Chasin' the Grateful Dead, no shoes on my feet
Beggin' in the parking lot for something to eat,
DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!
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MYSTIQUE
Say Hi to the elves for me.
Registered: 04/28/07
Posts: 1,764
Loc: Canada
Last seen: 4 years, 8 months
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: MYSTIQUE]
#7182695 - 07/16/07 10:11 AM (15 years, 10 months ago) |
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BTW the reason I had that list was that tetracycline is not good in getting rid of everyday colds and bacterias so what you would have on your hands day to day would not be that effected by the drug.
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Dont know what the fuck I just said? READ THIS
http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary
I ain't a hippy but I'm covered in dirt
Sippin lots of mushroom tea in a tye-dye shirt
Chasin' the Grateful Dead, no shoes on my feet
Beggin' in the parking lot for something to eat,
DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!
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figgusfiddus
Arrogant Worm
Registered: 02/02/07
Posts: 2,126
Loc: Figgus, Fiddia
Last seen: 14 years, 8 months
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: MYSTIQUE]
#7182725 - 07/16/07 10:29 AM (15 years, 10 months ago) |
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An antibiotic tends to kill off more than just the bacteria it's prescribed for. If a cake or casing had a severe bacterial infection, most antibiotics would work for treating it. But... bacteria aren't a major concern after birthing, unless you have some kind of problem with your fruiting environment (standing water, for instance).
Just grow shrooms, and stop worrying about problems you aren't having. It's not that hard to keep them from going bacterial.
Similarly, h2o2 is better for fixing problems than preventing them. Ever work with too much peroxide? Ever notice how it starts to damage your skin, making it peel a bit? Yeah, it'll do that to the shrooms too, at least on the cellular level. They do have enzymes to break it down, but that takes time, and a lot of lysis still occurs. It's just not smart. Wait until you have a problem to start fixing it.
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Edited by figgusfiddus (07/16/07 10:31 AM)
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pcubmycol
flattiefisherman
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: MYSTIQUE]
#7183448 - 07/16/07 01:56 PM (15 years, 10 months ago) |
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Quote:
MYSTIQUE said:
H202 will slow down growth and do more damage then good. It is only
10% H202 will not hinder growth at all
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weretheshit.com is the shit!
:-D
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figgusfiddus
Arrogant Worm
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: pcubmycol]
#7183791 - 07/16/07 03:30 PM (15 years, 10 months ago) |
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You mean 0.3% h2o2, assuming you're talking about a 10% solution of a 3% solution (3% is the common store-bought concentration).
Anyway, h2o2 isn't a preventative measure--it's a problem solver. It does delay growth briefly. How briefly is up to interpretation.
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xaxphaanes
Mycologist
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Posts: 2,988
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: pcubmycol]
#7183972 - 07/16/07 04:20 PM (15 years, 10 months ago) |
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Quote:
pcubmycol said:
Quote:
MYSTIQUE said:
H202 will slow down growth and do more damage then good. It is only
10% H202 will not hinder growth at all
It harms cube mycelium just like cobweb mycelium.
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"Anything i say is fictional"
what you should look for in manure
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hyphae
born to grow
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Loc: the rain forests
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: xaxphaanes]
#7184512 - 07/16/07 06:38 PM (15 years, 10 months ago) |
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Quote:
xaxphaanes said:
Quote:
pcubmycol said:
Quote:
MYSTIQUE said:
H202 will slow down growth and do more damage then good. It is only
10% H202 will not hinder growth at all
It harms cube mycelium just like cobweb mycelium.
Totally agree for those in doubt just watch it under an electron microscope. It will recover but why hinder growth at all?
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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal 
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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RogerRabbit
Bans for Pleasure
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: hyphae]
#7184797 - 07/16/07 07:52 PM (15 years, 10 months ago) |
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Furthermore, antibiotics are not used in bulk substrates because bacteria is not a contaminant of bulk. In fact, we pasteurize our substrates rather than sterilizing them so the bacteria can survive.
Contamination free growing is achieved by following proper sterile procedure during inoculations, and by air exchange during fruiting cycles.
RR
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Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
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Blutjager
Inhuman
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Posts: 9,220
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: shagg]
#7185355 - 07/16/07 09:27 PM (15 years, 10 months ago) |
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Quote:
shagg said:
h202 is unnecessary
Save it for cobweb an even than it only works if you get to the cobweb as soon as it shows up
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psiloglow
Stranger
Registered: 07/07/07
Posts: 115
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: MYSTIQUE]
#7185363 - 07/16/07 09:29 PM (15 years, 10 months ago) |
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Quote:
MYSTIQUE said:
If ya already think ya know the answer why even ask Mr.Doctor?
Because Doctors Can make mistakes and understand that they do not know everything. They are not quick to shout out answers to everything, like you. They research and learn the principles behind what they are studying. Period. No need to be hostile either...
Psiloglow--
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psiloglow
Stranger
Registered: 07/07/07
Posts: 115
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: MYSTIQUE]
#7185387 - 07/16/07 09:33 PM (15 years, 10 months ago) |
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Quote:
MYSTIQUE said:
BTW the reason I had that list was that tetracycline is not good in getting rid of everyday colds and bacterias so what you would have on your hands day to day would not be that effected by the drug.
BTW, everyday colds are caused by coronavirii, which are prokaryotic organisms (viruses) that will not respond to tetracycline, which effects the peptidoglycam of eukaryotic cells only. BTW.
PSILOGLOW---
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psiloglow
Stranger
Registered: 07/07/07
Posts: 115
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: xaxphaanes]
#7185420 - 07/16/07 09:39 PM (15 years, 10 months ago) |
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Xaxphannes? interesting name. The only one in the forum to give me even a simple answer. I appreciate it. Everyone here seems to be so over-confidant, I just wanna split. Kids I guess. So H2O2 would not hinder growth? I didn't think so considering it's basically oxygen and water, and fungus should appreciate that to a small extent...but about the Antibiotics. I thought it may be a good way to prevent/treat bacterial infection throughout the casing. I have a ton of tetracycline HCL , which is soluble in water to the extent of 100MG/1ML H2O. Thought about misting casings to keep em strong...dunno, may try on one to see. L8R.
PSILOGLOW..
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NStoney
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: psiloglow]
#7185672 - 07/16/07 10:29 PM (15 years, 10 months ago) |
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I use a little H202 in my misting bottle, just for fun. Somthing along the lines of 5-10% of 3% H202. Never had any problems with my Mycelium or Fruits when using this mix.
Anyways... back to my main point. H202 IS NOT just water and oxygen. Well sorta not. I'm no professional chemist, but if H202 will peel your hands then its obviously a little more than a harmless mix of water & oxygen. Here's my understanding of it....
Water is H20, and Atmospheric Oxygen is 02. Since peroxide is H202, what you really have is H20 with an extra single Oxygen molecule. Oxygen naturally occurs as O2, so this extra oxygen molecule on the H202 is very unstable and wants to leave the H202 mix and bond with other molecules; for example, another oxygen molecule perhaps (I do believe this is referred to as oxidation). This is why H202 doesn't keep well and is stored in an opaque container. This oxidizing effect is what kills spores and various other things and makes H202 useful to us mycologists.
So... Yes when H202 loses its Oxygen molecule one of the byproducts is water, but the oxidizing effect that the extra oxygen molecule provides is harmfull to many things including mycelium. Apparently living mycelium (and other living things) produce something that the oxygen molecule can bind to without destroying the mycelium, and this is its natural defense against oxidation, but this defense process takes some time so the mycelium cannot totally nutralize the oxidation, and some mycelium does die from H202 exposure, just not all of it. Afterward it is able to grow and recover. Think of H202 as liquid Ozone (O3).
Chemistry Guru's chime in here and please clarify/correct my attempt at explaining this.
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figgusfiddus
Arrogant Worm
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Posts: 2,126
Loc: Figgus, Fiddia
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: psiloglow]
#7185716 - 07/16/07 10:36 PM (15 years, 10 months ago) |
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Quote:
psiloglow said:
Xaxphannes? interesting name. The only one in the forum to give me even a simple answer. I appreciate it. Everyone here seems to be so over-confidant, I just wanna split. Kids I guess. So H2O2 would not hinder growth? I didn't think so considering it's basically oxygen and water, and fungus should appreciate that to a small extent...but about the Antibiotics. I thought it may be a good way to prevent/treat bacterial infection throughout the casing. I have a ton of tetracycline HCL , which is soluble in water to the extent of 100MG/1ML H2O. Thought about misting casings to keep em strong...dunno, may try on one to see. L8R.
PSILOGLOW..
If you don't want advice, don't ask for it. Most of the people here know just what they're talking about--way the hell more than you seem to, full of yourself as you are. I've seen this bullshit attitude in every thread you've posted so far, and I don't see what purpose you think you're serving by asking a question, then denying the answers when they come.
If you have to choose one person to listen to, make it RR. If you want answers that always coincide with what you already believe, ask your imaginary friends. Real people with real experience are likely to give you answers you apparently can't handle (simple though they are).
Enough of your "look at me, I know more than anyone" game--you have evidenced repeatedly that you don't know jack about cultivation compared to the people you're belittling, and you don't even seem to know jack about the tangential topics you are so eager to jump on. Though it doesn't matter, virii, including coronavirii, are not prokaryotes. Go back to school, my man. That doesn't mean they respond to antibiotics, which of course they don't because they're acellular, but virtually everyone knows (or should know) that anyway.
I know all this sounds harsh, but it's much easier when you just listen to the advice you asked for, or at least debate it sensibly rather than with arrogant "Pshaw, I know more than all these kids"-style remarks. People are giving you fundamental reasons why the methods you're suggesting won't work (e.g., bacterial contamination is not a problem with bulk because it is pasteurized, rather than sterilized), and you're pretending they don't mean anything by spouting completely unrelated solubility figures. Great work.
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Edited by figgusfiddus (07/16/07 10:43 PM)
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psiloglow
Stranger
Registered: 07/07/07
Posts: 115
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: figgusfiddus]
#7189671 - 07/17/07 07:23 PM (15 years, 10 months ago) |
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your right about virri not being pro-karyotic. I believe, its been a while since I had to cover such trivial pre doctoral material, I apologize. But the reason Virii do not respond to antibiotics varies, and has a lot to do with the fact that virii are comprised mainly of RNA (with the exception of a few). Not DNA. So stop pestering me, your a nuisance. Bye
Psiloglow-
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bzRooom
don't be stupid
Registered: 07/10/07
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: psiloglow]
#7189915 - 07/17/07 08:21 PM (15 years, 10 months ago) |
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@psiloglow-you are so wise.
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AbyssWP
Stranger
Registered: 05/30/07
Posts: 314
Loc: colorado
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: bzRooom]
#7190152 - 07/17/07 09:22 PM (15 years, 10 months ago) |
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hey psiloglow, can you please respond to my pm, its very important for my future 
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psiloglow
Stranger
Registered: 07/07/07
Posts: 115
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: bzRooom]
#7190161 - 07/17/07 09:23 PM (15 years, 10 months ago) |
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LOL...c'mon just funnin that guy. Hes a real joker. I Don't know everything, that's why I ask questions. I rely on the folks on this message board to provide knowledge, not ridiculous responses based out of hostility. Ya know?
Psiloglow--
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Boarders
Stranger
Registered: 08/19/06
Posts: 333
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: psiloglow]
#7191244 - 07/18/07 02:28 AM (15 years, 10 months ago) |
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I call troll.
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LVL12
Author andPerfector ofFailure
Registered: 04/09/07
Posts: 483
Last seen: 8 months, 4 days
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: Boarders]
#7191275 - 07/18/07 02:48 AM (15 years, 10 months ago) |
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psiloglow - will you teach me how to grow mushrooms?
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Disclaimer: Posts from LVL12 are strictly for entertainment and are a work of fiction, nothing written or said by LVL12 should in interpreted as truth, fact or by their literal definitions or translations. Anyone who reads/listens to these words does so at their own risk, parental discretion is advised.
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FellowGrower
Fungal Freedom Fighter
Registered: 05/25/07
Posts: 1,696
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: LVL12]
#7191343 - 07/18/07 03:25 AM (15 years, 10 months ago) |
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enough, enough, I've heard enough of this!
Stop asking stupid questions,
Stop making newbies feel stupid for asking them.
its like both people need to make changes.
I was thinking the other day, and CAN YOU IMAGINE?
having never grown shrooms... to grow em without the shroomery?
You can't ask a book questions. To grow shrooms is an art form past down thru generations of research. To master it, one need walk hand n hand with a "pro" for their first 2-3 cakes!
Don't get ahead of yourself in this hobby.
and try to preveiw at least just the title of your post and think to yourself... has this question already been answered somewhere else?
But I know how you are... you want your answers yesterday right?
b/c the stuff your asking about is the stuff that your currently doing! so, as much as I used to hate to hear it..."But, you need to read more Homie. (just a suggestion)
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LVL12
Author andPerfector ofFailure
Registered: 04/09/07
Posts: 483
Last seen: 8 months, 4 days
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: FellowGrower]
#7192842 - 07/18/07 01:51 PM (15 years, 10 months ago) |
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Quote:
FellowGrower said:
enough, enough, I've heard enough of this!
Stop asking stupid questions,
Stop making newbies feel stupid for asking them.
its like both people need to make changes.
I was thinking the other day, and CAN YOU IMAGINE?
having never grown shrooms... to grow em without the shroomery?
You can't ask a book questions. To grow shrooms is an art form past down thru generations of research. To master it, one need walk hand n hand with a "pro" for their first 2-3 cakes!
Don't get ahead of yourself in this hobby.
and try to preveiw at least just the title of your post and think to yourself... has this question already been answered somewhere else?
But I know how you are... you want your answers yesterday right?
b/c the stuff your asking about is the stuff that your currently doing! so, as much as I used to hate to hear it..."But, you need to read more Homie. (just a suggestion)
Am I still drunk? Or did you actually just make a few valid points? The whole goddamn world has gone topsy turvey now.
But one thing, asking stupid questions is fine, you'll most likely get a few derogatory responces but there will be some good honest answers to, but when you ask a question, then get the advice from some of the best growers on here, and then just argue with it? Its really irritating because it only proves you don't want advice you want some "yes" men to help boost your ego while you do a whole lot of stupid stuff. And for that, I have no sympathy when people start making you feel stupid, because at that point you need to be humbled before you can learn.
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Disclaimer: Posts from LVL12 are strictly for entertainment and are a work of fiction, nothing written or said by LVL12 should in interpreted as truth, fact or by their literal definitions or translations. Anyone who reads/listens to these words does so at their own risk, parental discretion is advised.
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figgusfiddus
Arrogant Worm
Registered: 02/02/07
Posts: 2,126
Loc: Figgus, Fiddia
Last seen: 14 years, 8 months
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: psiloglow]
#7192854 - 07/18/07 01:57 PM (15 years, 10 months ago) |
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Quote:
psiloglow said:
your right about virri not being pro-karyotic. I believe, its been a while since I had to cover such trivial pre doctoral material, I apologize. But the reason Virii do not respond to antibiotics varies, and has a lot to do with the fact that virii are comprised mainly of RNA (with the exception of a few). Not DNA. So stop pestering me, your a nuisance. Bye
Psiloglow-
Hey man, you brought it up. If you're going to try to throw around material like you're a genius, you'd might as well at least be right.
Additionally, the reason viruses do not respond to antibiotics doesn't vary. They're not bacteria. Their method of reproduction is completely different, along with everything else about them. Aside from making people ill, they have nothing, nothing in common with bacteria. It has nothing to do with RNA vs. DNA, which you just pulled out of your ass.
Antibiotics work by disrupting the integrity of the cell wall during fission, if I remember correctly. It's been almost a decade since I've studied this, which is why I've got to assume you've never studied it if you don't know this. Viruses don't undergo fission, or anything like it. They don't have cell walls. They are acellular.
This is a total non sequitur, and it's going away now. Let it be a lesson though that you, phD or no, could not pass an undergraduate (or high school) bio test with the knowledge you know now, and shouldn't be lecturing people on such topics. You definitely shouldn't be lecturing them on mushroom cultivation. Be humbled, and maybe you'll learn something.
Have a good one.
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Edited by figgusfiddus (07/18/07 02:05 PM)
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Pooter
Shroom Mongler
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Posts: 146
Loc: With waldo.
Last seen: 7 years, 10 months
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: hyphae]
#7192855 - 07/18/07 01:58 PM (15 years, 10 months ago) |
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I'm pretty sure SEMs require a vacuum to operate. If you put a culture of cube myce in a SEM, it's going to be dead, aka not a good test environment.
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FellowGrower
Fungal Freedom Fighter
Registered: 05/25/07
Posts: 1,696
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: LVL12]
#7195297 - 07/18/07 11:08 PM (15 years, 10 months ago) |
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Am I still drunk? Or did you actually just make a few valid points? The whole goddamn world has gone topsy turvey now.
...agreed. thats why I'm no longer "GoldenSchlager" but just "a FellowGrower" ... just trying to get my grow on! before I was...
"The Schlager" ... just some arrogant punk with his head so far up his ass he could wear it as a hat!
and so yeah! let it be known. The shroomery can (and will) humble a mutherFker!
...like no other!
people will verbally and Visually ASSAULT YOU, until you realize, like you're just a tiny microscopic flake of frozen flea shit.
...since Ive tripped I've had a new found respect and love for the shroomery!
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[url=http://files.shroomery.org/files/15-009/514830879-Take_over_this_thread.jpg]
Edited by FellowGrower (07/18/07 11:10 PM)
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xaxphaanes
Mycologist
Registered: 08/08/05
Posts: 2,988
Last seen: 9 months, 21 days
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: psiloglow]
#7195396 - 07/18/07 11:25 PM (15 years, 10 months ago) |
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Quote:
psiloglow said:
Xaxphannes? interesting name. The only one in the forum to give me even a simple answer. I appreciate it. Everyone here seems to be so over-confidant, I just wanna split. Kids I guess. So H2O2 would not hinder growth? I didn't think so considering it's basically oxygen and water, and fungus should appreciate that to a small extent...but about the Antibiotics. I thought it may be a good way to prevent/treat bacterial infection throughout the casing. I have a ton of tetracycline HCL , which is soluble in water to the extent of 100MG/1ML H2O. Thought about misting casings to keep em strong...dunno, may try on one to see. L8R.
PSILOGLOW..
Quote:
xaxphaanes said:
It harms cube mycelium just like cobweb mycelium.
what you just said and what i said contradicts itself bro.
--------------------
"Anything i say is fictional"
what you should look for in manure
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mycohobbiest
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Registered: 12/22/11
Posts: 14
Last seen: 10 years, 9 months
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: hyphae]
#16208716 - 05/10/12 06:25 AM (11 years, 27 days ago) |
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diluting 3% hydrogen peroxide 1/10 just gets things wet. use at full 3% strength for treating cobweb, but only if necessary.
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: Misting the Casing? How often H2O2 OK in the mix? [Re: psiloglow]
#16208873 - 05/10/12 06:25 AM (11 years, 27 days ago) |
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 This thread has been closed.
Reason:
I'm not sure we needed to bump a 4 year old thread to state the obvious.
RR
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