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OfflineNickio
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What is the timeframe for LC?
    #7180849 - 07/15/07 08:44 PM (16 years, 7 months ago)

How long will it take from the day innoc'ing LC till it is usable to knock up other jars, and how long it takes a jar to colonize from LC (I've heard its faster than spore syringes). Thanks :laugh:


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Offlinecheor
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Re: What is the timeframe for LC? [Re: Nickio]
    #7180947 - 07/15/07 09:06 PM (16 years, 7 months ago)

my lc had a fair bit of mycelium on the 3rd day using honeywter should be usable soon as u can see the myc long as u can suck it up in a syringe


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Offlineshroom1957
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Re: What is the timeframe for LC? [Re: Nickio]
    #7180953 - 07/15/07 09:09 PM (16 years, 7 months ago)

As with everything else, it depends on temperature, amount of inoculant, quality of LC, etc.
My first experience was last monday, took until friday before I saw any 'obvious' growth. That was at about 75-80 F.
I inoculated with a B+ syringe so no advantage in time there, the advantage is from going from LC to the next step.
I knocked up some PF style cakes last evening so we'll see if the mycellium was developed enough to give an advantage going to cakes.
I'd wait a lot longer than I did to use the mycelia before I tried to do cakes, I was just experimenting. Probably when the LC is about half colonized with floating myelium.
I'l keep you posted, but it would probly be prudent to wait a week or so after growth is established, maybe more.

The advantage would seem to be that although it takes a multispore inoculation as long to colonize LC as it does rye or cakes or wbs, the fact that you've got all that mycellium floating around in a liquid culture that you can then use to knock up solid substrate with and multiply your efforts.

I'm sure someone will have a better answer but that's where I'm at.


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