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simplystoned
Tree Wizard
Registered: 03/28/07
Posts: 865
Loc: K-Town up in this biache
Last seen: 16 years, 4 months
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Sterilizing Needles
#7177320 - 07/15/07 12:27 AM (16 years, 8 months ago) |
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Hey,
So in a pinch tonight I decided to sterilize my needles. My process was this:
Soak needles in bleach water for 15 minutes Wash all the bleach off and suck in water/spray out water for a while I took the ends of a PP bag and using my impulse sealer I sealed little baggies for each individual needle. I then took the needle packets and put them in a canning jar and PC'd for an hour.
Think they'll be sterile? I ALWAYS use fresh needles, so I don't have much experience with this.
-------------------- "Your pain is the breaking of the shell which encloses your understanding. It is the bitter potion by which the physician within you heals your sick self. Therefore trust the physician, and drink his remedy in silence and tranquility: For his hand, though heavy and hard, is guided by the tender hand of the Unseen, And the cup he brings, though it burn your lips, has been fashioned of the clay which the Potter has moistened with His own sacred tears." - Kahlil Gibran
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figgusfiddus
Arrogant Worm
Registered: 02/02/07
Posts: 2,126
Loc: Figgus, Fiddia
Last seen: 15 years, 5 months
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Uh, yes.
I used the same needle for months, for many, many grows, with 100% success when I first started. All I did to sterilize was suck in alcohol, expel, and suck in a few exchanges of freshly-boiled water. Then I sucked up the inoculant and went about my business.
People make such a huge deal out of syringe sterilization! Your contaminants aren't going to survive thirty seconds of 90% isopropyl exposure any more than the shrooms would.
Your needles were effectively sterile before you PCed them. People always seem to focus on the least important aspects of sterility... tainted (or semi-tainted) inoculant is a far more common cause of contamination, along with poor substrate conditions, than an understerilized needle. Alcohol does the trick for me, no question.
-------------------- FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS
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roquet
Expat tippler
Registered: 05/29/07
Posts: 1,195
Loc: Dubai بجدية عربي...
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is just boiling it a few mins then sucking up and squirting out boiling water 2 or 3 times ok?
Instinctively I feel boiling is enough but then we do PC the jars so maybe not.
Anyone know for sure?
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 29 days
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Re: Sterilizing Needles [Re: roquet] 1
#7178410 - 07/15/07 08:05 AM (16 years, 8 months ago) |
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All that is fine prior to the first inoculation. You should then flame sterilize between each jar to prevent cross-contamination between jars.
Just a note here too: 70% alcohol will do a better job of killing off contaminants than 90% or 99% alcohol. The reason is that the cell wall is tricked by the water in the alcohol into admitting it into the interior of the cell, where the cell is then destroyed as the alcohol evaporates back out. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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The shroomy 1
Luminous beings surround me
Registered: 03/27/07
Posts: 5,543
Loc: The Aether
Last seen: 6 months, 23 days
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Re: Sterilizing Needles [Re: roquet]
#7178413 - 07/15/07 08:06 AM (16 years, 8 months ago) |
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Remember, that those are apples and oranges. When you deal with nutrative substrates versus equipment. Your needles and syringes are not made up of organic material that contams will feed off of.
-------------------- AMU Q&A thread.
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figgusfiddus
Arrogant Worm
Registered: 02/02/07
Posts: 2,126
Loc: Figgus, Fiddia
Last seen: 15 years, 5 months
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Re: Sterilizing Needles [Re: RogerRabbit]
#7179938 - 07/15/07 04:51 PM (16 years, 8 months ago) |
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Quote:
RogerRabbit said:
Just a note here too: 70% alcohol will do a better job of killing off contaminants than 90% or 99% alcohol. The reason is that the cell wall is tricked by the water in the alcohol into admitting it into the interior of the cell, where the cell is then destroyed as the alcohol evaporates back out. RR
That's right, I'd forgotten you'd said that.
Half the time I can only find 70% anyway, so all the better.
-------------------- FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS
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