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shroomben2003
Shroomer
Registered: 05/30/03
Posts: 385
Loc: Some Shroom Pasture
Last seen: 4 years, 9 months
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Q-tip Technique
#7168260 - 07/12/07 09:02 PM (16 years, 8 months ago) |
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I have searched the forums about using a q-tip for inoculation rather than syringes and have come accross some useful information. I just want to get all of this straight now. I can take q-tips, boil them in water for about 5 minutes. Then I quickly take them out while wearing gloves and drop them into a ziplock bag. While these q-tips are still somewhat damp from the boiling water, I can take the spore prints on aluminimu foil and quickly drop them in the bag and seal it again. I can then manipulate the q-tip to "soak up" or allow the spores from the foil to stick to it. I can then use these q tips to quickly drop into grow jars that are waiting to be inoculated. I know there are probably some holes in my theory but I don't have a glovebox or a pc. I would also much rather do this than making a syringe. Any help would be appreciated.
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sHrOoMaCiDe
I lie in thesoil andfertilizemushrooms
Registered: 02/09/07
Posts: 247
Loc: Gliese581c
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I've never heard of this before, but it sounds like it really could work. In my experience, i usually have contamination problems when using syringes. The q-tip idea sounds cool. Give it a try and tell us how it goes. I mean as long as you boil it good and keep it from getting contaminated, then i don't see why it wouldn't work ya know?
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LVL12
Author andPerfector ofFailure
Registered: 04/09/07
Posts: 483
Last seen: 1 year, 5 months
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Sounds like it could work, but also sounds innefficiant. Making syringes is super easy, getting syringes is super easy too, (any feed or medical supply store should have them) but if you have something against the syringes, go for it, just seems like you'll have trouble with measurements and quanities and what nots... What are you trying to knock up?
-------------------- Disclaimer: Posts from LVL12 are strictly for entertainment and are a work of fiction, nothing written or said by LVL12 should in interpreted as truth, fact or by their literal definitions or translations. Anyone who reads/listens to these words does so at their own risk, parental discretion is advised.
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LVL12
Author andPerfector ofFailure
Registered: 04/09/07
Posts: 483
Last seen: 1 year, 5 months
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Re: Q-tip Technique [Re: LVL12]
#7168397 - 07/12/07 09:23 PM (16 years, 8 months ago) |
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NO glove box or PC? so I'm assuming you're going to use PF cakes, also you're going to have to open the BRF cake after its sterilized to insert the q-tip, doing this without a glove box is a big contam risk. Also, you'll have to bypass the vermiculite layer,
this seems like its most likely going to be contam city, if it works. So, do you dislike the syringes or do you have trouble getting access to them?
-------------------- Disclaimer: Posts from LVL12 are strictly for entertainment and are a work of fiction, nothing written or said by LVL12 should in interpreted as truth, fact or by their literal definitions or translations. Anyone who reads/listens to these words does so at their own risk, parental discretion is advised.
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shroomben2003
Shroomer
Registered: 05/30/03
Posts: 385
Loc: Some Shroom Pasture
Last seen: 4 years, 9 months
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Re: Q-tip Technique [Re: LVL12]
#7168420 - 07/12/07 09:30 PM (16 years, 8 months ago) |
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I don't know, it seems easier just to do it with q-tips although the risk for contamination is high. Maybe I'll just get syringes. Can you get them at petsmart?
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euphoricpoison
Expand your Mind
Registered: 10/10/06
Posts: 3,270
Loc: NewYork
Last seen: 4 years, 12 days
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It sounds really risky, you are exposing it to a lot of contams. When reading this, I felt that it might worked, however, when I read that you do not have a glove box, it is gonna be even more exposed to contams. I would spend the extra cash and do it with a spore syringe it is not hard to do and safer then the Q-tip.
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euphoricpoison
Expand your Mind
Registered: 10/10/06
Posts: 3,270
Loc: NewYork
Last seen: 4 years, 12 days
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You can get them at walmart, cvs, and a lot of other places, do a search.
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shroomben2003
Shroomer
Registered: 05/30/03
Posts: 385
Loc: Some Shroom Pasture
Last seen: 4 years, 9 months
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0k thanks a lot. If they ask, I'll just tell them I need them for a biology course I'm taking.;)
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euphoricpoison
Expand your Mind
Registered: 10/10/06
Posts: 3,270
Loc: NewYork
Last seen: 4 years, 12 days
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Yeah, if they ask you can tell them whatever you want. Even saying that you are making a spore syringe will be fine. GL
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LVL12
Author andPerfector ofFailure
Registered: 04/09/07
Posts: 483
Last seen: 1 year, 5 months
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They don't ever ask, people buy syringes all the time, sometimes cause they're diabetic (although the syringes we use would be very large for that) sometimes for heroin or other drugs, sometimes for their pets. Usually whenever someone asks me why i am purchasing something, I tell them its for drugs, then laugh, they laugh too. The best was the bird seed, 50lbs bag, at home depot, Girl says "lots of birds at your house eh" I said "no, I turn the bird seed into drugs." She laughed and talked about sudafed for a second. Its funny.
They won't ask you why you want them, and if they do, just tell them its private, or tell them botany or biology or something.
The only time you may have trouble getting syringes is if you're underage. but even then, where theres a will there is a way.
-------------------- Disclaimer: Posts from LVL12 are strictly for entertainment and are a work of fiction, nothing written or said by LVL12 should in interpreted as truth, fact or by their literal definitions or translations. Anyone who reads/listens to these words does so at their own risk, parental discretion is advised.
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SuperFunGuy
1up farmer
Registered: 07/10/07
Posts: 786
Loc: Michigan
Last seen: 12 years, 2 months
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I would say you should try it. But all in all to go with syringes. It's a good enough set up already. But, thats not saying it cant be improved, just this isn't the way to improve it IMO. But no way to tell with out trying it eh? Log your results or a jar or so.
FunGuy
-------------------- FunGuy Bored? Pissed? Have a question? Feel free to PM me =] Fuck Bill O'Reilly Omfg! I'm invisible!! “If you ever start taking things too seriously, just remember that we are talking monkeys on an organic spaceship flying through the universe.” - Joe Rogan
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