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Offlineeidolon
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Cob Web Mold?
    #7162539 - 07/11/07 09:10 PM (14 years, 6 months ago)

I just started my first grow, out of 11 jars I only notice growth in two, but I think I may have a cob web contamination. Is this normal inside the jars? from what I've read it's not too common.

I will post pics in a bit.

I also was wondering, after sterilization I've kept the tinfoil on the jars only removing it to inoculate, I have nothing else over the holes in the jars but tinfoil.

Also, how long does one have to let the jars cool after sterilization? I'm thinking nothing's growing in some of the other jars because I inoculated too hot? If this is the case, what can I do? Re-inoculate the jars? Should I re-sterilize or should I start a whole new substrate? How long should I wait for PC before I make a decision?

I'm basically using the PF tek from the RR videos :smile:


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OfflineDr_T
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Re: Cob Web Mold? [Re: eidolon]
    #7162604 - 07/11/07 09:23 PM (14 years, 6 months ago)

Probably mycelium.

Let your jars cool to room temperature. You didn't say, but I'm guessing you used a spore syringe? And how long have you been waiting?

Oh, and congrats on your first grow!


--------------------
Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.


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Offlineeidolon
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Re: Cob Web Mold? [Re: Dr_T]
    #7162638 - 07/11/07 09:33 PM (14 years, 6 months ago)

And thanks for the quick response!

Yes, I'm using a spore syringe, I've been waiting, lets see, 66 hoursish?

I did not let all the jars cool to room temperature, can I reinoculate if no growth occurs? should I resterilize or just trash the substrate and start over? Sucks, I used half my syringe at least!

The reason I'm thinking it might be cobweb is because there are a few white specks that are a bit brighter but I can't really tell yet, I have to wait for my roomie to get home so I can take some pictures.

I also ran out of BRF while making my substrate and substituted it with white rice for the last few jars, good, bad, ugly?

Also, what's a good level of water to use? The RR video I believe stated 1 cup brf, 1 cup water, 2 cups verm, but from what I've read my substrate is probably too wet.

And again: I also was wondering, after sterilization I've kept the tinfoil on the jars only removing it to inoculate, I have nothing else over the holes in the jars but tinfoil. Is this OK or what is better?

thanks in advance guys!


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OfflineDr_T
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Re: Cob Web Mold? [Re: eidolon]
    #7162684 - 07/11/07 09:44 PM (14 years, 6 months ago)

66 hours?

Wait like a week before you decide there's no growth. :smile:
White specks likely are mycelium. :thumbup: It's fluffy and white like a cotton ball. It's not uncommon to have some spore sprout early, so it could be. Wait and see.

White rice is not ideal, you may have problems with those. I don't know much about cakes in general, so I can't comment on the water- but if you followed RR's recipe, then trust it.

Leave the foil if you don't use micropore tape or a breather hole with polyfill. Supposedly the top laer of verm protects, though. So more air might be better. Again, I'm not a cakeman.


--------------------
Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.


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OfflineDr_T
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Re: Cob Web Mold? [Re: Dr_T]
    #7162869 - 07/11/07 10:16 PM (14 years, 6 months ago)

Oh, and read up on Liquid Culture. Use a little of your remaining spores (like 1mL) to make some. Once you get that, you have an easier time with the jars.

But you need a pressure cooker to do it.


--------------------
Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.


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Offlinedeformedreality
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Re: Cob Web Mold? [Re: eidolon]
    #7163173 - 07/11/07 11:11 PM (14 years, 6 months ago)

need a pressure cooker for lc? ive made three lc's from boiling water with a jar in it, pulling it out with 3/4 jar of water and stirring in a teaspoon of honey and steaming it for 30 minutes and they all produced great lc's and none of the jars inoculated with it have contaminated.


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Offlineeidolon
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Re: Cob Web Mold? [Re: Dr_T]
    #7163178 - 07/11/07 11:12 PM (14 years, 6 months ago)

I'll read up on the liquid, I don't have a pressure cooker though.

After further examination, I'm almost convinced it is indeed cobweb mold, I will wait a bit longer, but is there any way to recover when this happens inside the jars?

The white dots which appear to be mycelium are a brighter white and different texture than what I believe to be is the mold, also, tiny specs of white dots have appeared on another jar without what appears to be the mold.

What can I do to prevent this in the future? Can I re-use the jars after boiling them and cleaning them and replacing the substrate?

You guys are very helpful! I can't wait until the future!


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Offlineeidolon
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Re: Cob Web Mold? [Re: deformedreality]
    #7163335 - 07/11/07 11:41 PM (14 years, 6 months ago)

Quote:

deformedreality said:
need a pressure cooker for lc? ive made three lc's from boiling water with a jar in it, pulling it out with 3/4 jar of water and stirring in a teaspoon of honey and steaming it for 30 minutes and they all produced great lc's and none of the jars inoculated with it have contaminated.




The more I read about this, the more it seems like a good idea I can accomplish without leaving my house, and almost enticing, magnetic stirrer? You mean, something I can actually build with all the computer parts I'm to nostalgic about to throw out?


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OfflineDr_T
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Re: Cob Web Mold? [Re: eidolon]
    #7163731 - 07/12/07 12:59 AM (14 years, 6 months ago)

If you have contamination, then good sterile technique is your weakness. A pressure cooker can help with that, but it's possible to grow without one. Still, it's easier with. But technique is important too.

The magnetic stirrer is just a start, there's a whole range of fun items to build if you go to different styles of growing. PF Tek is one way, but there are others. :laugh:


--------------------
Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.


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Offlineeidolon
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Re: Cob Web Mold? [Re: Dr_T]
    #7164057 - 07/12/07 01:44 AM (14 years, 6 months ago)



and there we have it ladies and gentlemen.
(right click->view image for full-size, apparently the forums auto resize, good job!)


Edited by eidolon (07/12/07 01:44 AM)


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Offlineeidolon
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Re: Cob Web Mold? [Re: eidolon]
    #7164369 - 07/12/07 02:50 AM (14 years, 6 months ago)



another pic


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Offlineeidolon
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Re: Cob Web Mold? [Re: eidolon]
    #7165017 - 07/12/07 09:41 AM (14 years, 6 months ago)

+12h or so there's a lot more activity in the jars but I wont be able to post a pic for another 12h or so (roomies cam)..


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OfflineRogerRabbitM
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Re: Cob Web Mold? [Re: eidolon]
    #7165160 - 07/12/07 10:41 AM (14 years, 6 months ago)

You have several types of contamination at work there. Toss that out! If you need to save the glass part of the jar, boil it for two hours before opening to clean that mess out.
RR


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Offlineeidolon
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Re: Cob Web Mold? [Re: RogerRabbit]
    #7165846 - 07/12/07 02:01 PM (14 years, 6 months ago)

Quote:

RogerRabbit said:
You have several types of contamination at work there. Toss that out!  If you need to save the glass part of the jar, boil it for two hours before opening to clean that mess out.
RR




Such as?
:frown:


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Offlineeidolon
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Re: Cob Web Mold? [Re: eidolon]
    #7165933 - 07/12/07 02:19 PM (14 years, 6 months ago)

And what is contam and what is bad?

Looking at that jar today, some of that has started to turn green, definitely not good. I'm not sure I have any jars that are growing and not contaminated, which really pisses me off, I was so anal about sterile technique that I might as well give up until I move somewhere else :frown:


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OfflineDr_T
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Re: Cob Web Mold? [Re: eidolon]
    #7165979 - 07/12/07 02:31 PM (14 years, 6 months ago)

If you are using good sterile technique, including a glove box and flaming the needle and everything, then your substrate is contaminated.

A pressure cooker makes everything easier, but the point of PF Tek is that you shouldn't need one. But either way, if you aren't introducing contams, then they are already there. So boil longer, at least.

Edit- you can find a cheap pressure cooker if you look around.


--------------------
Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.


Edited by Dr_T (07/12/07 02:32 PM)


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Offlineeidolon
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Re: Cob Web Mold? [Re: Dr_T]
    #7166083 - 07/12/07 03:03 PM (14 years, 6 months ago)

Walmart has one for a good price, I might have to grab one.

Here's a review of my S-tek:

- Mixed substrate, seemed a little damper than it should be but I used the portions from the RR video.

- Capped jars upside down with holes in lids, covered with tinfoil, boiled for 90 minutes.

- Sterilized bathroom by spraying bleach solution, cleaning everything, washed the floor, etc. Bleeched a few times over a few hours.

- Removed jars from boiling to clean room, let cool.

- Washed gloves with bleech (didn't use alcohol because I was using a propane torch and I didn't want to set my hands on fire)

- Put jars in glovebox, remove foil, inoc (flamed needle every jar before and after), replace foil

- Incubate.

Now, I never used any tape over the holes, partly because I don't have any and partly because I'm confused as to when to apply it, from what I can see in the video it gets applied before inoculation, but, wouldn't the needle just put a hole in the tape and make it useless, or am I all wrong?

I believe you are right Dr_T, that the substrate must've been contaminated because I numbered all the jars and I don't think it's a cross contamination, what are the chances of contamination after inoculation, and how does one control those? tape/tinfoil info would apply here.

My first jar was inoculated before the substrate cooled, nothing appears to have grown in it so far so I gave it a few more ml of spores. I hope this was not a waste.

Is there anything I can do to save my spores before I run out? I don't suppose multiplying spores could be as easy as injecting some agar? or something? excuse my newbish accent :wink:

I also wanted to say thanks to RR, not only do you make a video so I can do this, you reply to my posts on interweb forums to help me out, I will definitely be buying the DVD when I can afford it! You deserve it!

thanks guys


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OfflineCaptainLinger
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Re: Cob Web Mold? [Re: eidolon]
    #7166103 - 07/12/07 03:09 PM (14 years, 6 months ago)

Actually, once you've seen the DVDs...*you* deserve it.


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Offlineeidolon
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Re: Cob Web Mold? [Re: CaptainLinger]
    #7166135 - 07/12/07 03:17 PM (14 years, 6 months ago)

Quote:

CaptainLinger said:
Actually, once you've seen the DVDs...*you* deserve it.




not sure what you meant, are you implying that they're just that good?


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OfflineDr_T
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Re: Cob Web Mold? [Re: eidolon]
    #7166163 - 07/12/07 03:25 PM (14 years, 6 months ago)

Basic technique sounds good.

I haven't tried cakes, I do grain jars, but the idea is the same.

I use regular tape over the injection holes, but I make breather holes with polyfil.
Peel back the foil, wipe with alcohol.
Leave alcohol pad in place while flaming the needle.
Make the injection, then cover the hole with the alcohol pad while I put the needle down.
Recover with foil, set the jar aside.
Repeat until all jars are done.

Now, the tape.
Alcohol on each jar will have dried.
Peel a piece of tape- don't touch the part you are putting over the hole.
Peel back foil, place tape. Remove foil.

But you aren't supposed to need all that with PF Tek, the verm layer stops contams. So it may be extra, but it won't hurt.

Your best bet at this point is to discard the bad stuff and start over. I wouldn't throw out the jars, I'd boil them, then dump out the bad stuff, wash and reboil. But I'm thrifty like that. It's up to you. Supposedly you don't need a pressure cooker, either...


--------------------
Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.


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