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Offlinesutec
addict
Registered: 09/05/99
Posts: 301
Last seen: 14 years, 8 months
Re: Sod the cakes, what about straight to casing
    #71388 - 01/12/00 11:38 AM (24 years, 1 month ago)

Casing is generally a non-nutritive substance (Vermiculite, peat, potting soil) and fungi don't do that well in those substances. It sounds like you have some sterility issues.

Where did you obtain the spores from? It's possible the syringe was/is contaminated.

How long did you pressure cook the jars for?
I'd recommend at least an hour at 15PSI (make sure your pressure cooker can reach 15psi, mine can only go to 12, so I have to add 20% to the steriliation time).

You said you noticed a funny smell, did you open the jars? Once pressure cooked you should only be removing the tape from the lid to innoculate with spores and then re-tape.

Sounds like you just need to be more sterile in your process. (wear a mask over your face, shower before beginning, drench your work room/area in Lysol or a 10% bleach/water solution).

Sterility is godly.



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InvisibleCLuB99
lost somewhere in time and space
Male User Gallery

Registered: 10/26/99
Posts: 1,316
Loc: my mind
Re: Sod the cakes, what about straight to casing [Re: sutec]
    #71390 - 01/12/00 11:48 AM (24 years, 1 month ago)

:rolleyes:
Don't try this....you'll have a 99% contaminated case....the reason is that casings are less resistant to contams than cakes..
Just be patient..and try again....
How do you sterilize your jars? Probably you need a pressure cooker...
Anyway today i toss :mad: :mad:TWENTY :mad: :mad: jars....and i USE a pressure cooker

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Anonymous

Re: Sod the cakes, what about straight to casing [Re: sutec]
    #71391 - 01/12/00 05:47 PM (24 years, 1 month ago)

I wouldn't try it either, for the same reasons they already mentioned - the casing doesn't provide nutrition, and is more indicive to contaminants than a cake. When growing, cleanliness is next to godliness. Just be patient, and take a few more precautions - it'll pay off. Good luck! :biggrin:

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Anonymous

Re: Sod the cakes, what about straight to casing [Re: sutec]
    #71392 - 01/12/00 06:29 PM (24 years, 1 month ago)

I gave this a go, with 2 containers. One got this brilliant green fungus to go with the white mycilia, the other I have just birthed, but because I couldn't see how far it was colonised, I had to throw out half of it. Stick with the jars, at least you know when they are fully colonised :wink:

jC

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Hungry, Cold, and Hunted - BWP


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OfflineTriGuy
Skeptic

Registered: 08/27/99
Posts: 105
Loc: Georgia
Last seen: 21 years, 5 months
Re: Sod the cakes, what about straight to casing [Re: sutec]
    #71393 - 01/12/00 11:08 PM (24 years, 1 month ago)

MS,

Sorry to hear of the failure. It seems I was lucky and my first attempt was by the book (PF Tek) and worked surprisingly well. Since then my success has been sporadic, and I'm starting to realize that cleanliness at the very beginning is essential.

It is worth the effort and we all must find a method that works for us. Look into the liquid growth medium for speed. There are people here who have it mastered and have been doing it for many years. I think they almost have a dedicated super sanitary lab set up in a room of their house and a set of materials and routines that work all the time. The one thing they all seem to have in common is a pressure cooker to prepare the substrate in.

Good luck and good night.

[This message has been edited by TriGuy (edited January 13, 2000).]


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Anonymous

Re: Sod the cakes, what about straight to casing [Re: sutec]
    #71394 - 01/13/00 01:28 AM (24 years, 1 month ago)

Club:

casings are NOT less resistant to contams than cakes. they are far more resistant because there is far less nutritious material for the contams to grow on. if you left uncolonized cakes sitting exposed they'd contaminate in no time compared to the casing. it's the same reason you wouldn't just inject spores into your casing... there's much less nutrition, therefore slower growth.. probably none at all from the spores. mold would beat it to the punch no problem on an exposed medium

agent orange


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