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PJDIDDLE


Registered: 10/17/02
Posts: 2,837
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Re: Germination Experiment: findings may surprise u. *DELETED* *DELETED* [Re: xaxphaanes]
#7076242 - 06/21/07 04:57 PM (16 years, 8 months ago) |
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Post deleted by ScriptReason for deletion: .
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xaxphaanes
Mycologist



Registered: 08/08/05
Posts: 2,988
Last seen: 1 year, 6 months
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Re: Germination Experiment: findings may surprise u. [Re: PJDIDDLE]
#7076250 - 06/21/07 04:58 PM (16 years, 8 months ago) |
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yea my grain jars or even my pf jars only take max 14 days so i dont see the gain
-------------------- "Anything i say is fictional" what you should look for in manure
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Helltick
Player


Registered: 10/30/05
Posts: 684
Loc: Deep...
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Re: Germination Experiment: findings may surprise u. [Re: xaxphaanes]
#7076287 - 06/21/07 05:05 PM (16 years, 8 months ago) |
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I love amazons...killer strain.
-------------------- My Agar Tek. My Greenhouse Tek. "It's not good when it's done, it's done when it's good"
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Dr_T
Random Dude




Registered: 06/03/07
Posts: 1,839
Loc: Colorado
Last seen: 16 years, 5 months
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Re: Germination Experiment: findings may surprise u. [Re: Helltick]
#7076378 - 06/21/07 05:21 PM (16 years, 8 months ago) |
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Quote:
Helltick said: I love amazons...killer strain.
Fuck yeah. Aggressive as hell.
-------------------- Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.
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hyphae
born to grow



Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 9 months
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Re: Germination Experiment: findings may surprise u. [Re: xaxphaanes]
#7077101 - 06/21/07 08:06 PM (16 years, 8 months ago) |
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Quote:
xaxphaanes said: am i wrong or isnt the 14 day mark normal time period that a jar should colonize ? whether they are new or old?
New or old as in spores?
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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xaxphaanes
Mycologist



Registered: 08/08/05
Posts: 2,988
Last seen: 1 year, 6 months
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Re: Germination Experiment: findings may surprise u. [Re: hyphae]
#7077113 - 06/21/07 08:10 PM (16 years, 8 months ago) |
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old spores didnt really take that long maybe 2-3 years old or so,newer is faster but not by much ime,lol i couldnt tell a diff really heh heh
-------------------- "Anything i say is fictional" what you should look for in manure
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hyphae
born to grow



Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 9 months
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Re: Germination Experiment: findings may surprise u. [Re: xaxphaanes]
#7077208 - 06/21/07 08:27 PM (16 years, 8 months ago) |
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You talking syringes or prints? I've had great luck with old syringes never a problem but prints have been another story. The surface of a spore is one of the hardest substances known to man if you look under a microscope you will see how they shrivel over time, I've personally seen spores 10yrs old take 2 weeks to germinate I've heard even three weeks from some cultivators. Most of us now have availability so time isn't or shouldn't be a problem.
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xaxphaanes
Mycologist



Registered: 08/08/05
Posts: 2,988
Last seen: 1 year, 6 months
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Re: Germination Experiment: findings may surprise u. [Re: hyphae]
#7077230 - 06/21/07 08:32 PM (16 years, 8 months ago) |
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well the old ones were takin from a print i had in a book and made a syringe and innoc'd about 24 hours later to let rehydrate.i could see it taking a long time with very old spores like you said 10 years lol (rr said he had a hard time with redboy which is 20-25 years heh) but with my little collection i havent seen a big diff..oh also theses are edibles (shouldnt make a diff though)
-------------------- "Anything i say is fictional" what you should look for in manure
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: Germination Experiment: findings may surprise u. [Re: xaxphaanes]
#7077405 - 06/21/07 09:16 PM (16 years, 8 months ago) |
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I've seen germination on a microscope slide within 20 minutes when fresh spores were used. About one spore out of one hundred will germinate when fresh, meaning 99% are duds. By one year, that ratio drops to less than one spore out of a thousand that will germinate. It goes downhill from there. With multispore inoculation, you often use several million spores, so you up the chances of the few that survive being in the mix. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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PinheadX
Stranger thanyou



Registered: 04/25/07
Posts: 1,414
Loc: TX Gulf Coast
Last seen: 6 years, 2 months
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Re: Germination Experiment: findings may surprise u. [Re: RogerRabbit]
#7077823 - 06/21/07 11:12 PM (16 years, 8 months ago) |
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hell, I'm freaked out by my latest experiment...
I took the spore coated rings off the stems of two mushrooms I grew outside on cowpoo, put them in a shotglass with aquafina that had been drunk out of, and happened to rinse some spores off of my FINGER into the shot glass as well. Covered with a tyvek disk I cut earlier, and in a DAY, had cloudy growth in the bottom of the shot glass.
I made a sterile (200 ml) honey water solution in the microwave, sucked up the liquid from the shotglass with a sterilized oral syringe and injected it into the honey water. Myc is growing RAPIDLY in the honey water over the past two days, sitting in light on top of my computer monitor.
unless I'm growing something else in the water, I'm amazed at the heartyness (is that a word?) of this particular bit of fungal material. I'm pretty sure this is mushroom myc, as it's also growing off the bits of purplish ring remnants that ended up in the honey water solution. I plan on PCing some jars of BRF+verm and injecting some of the LC in them to see what comes of it. Oh yeah, these are nice potency too... at least their parents were.
-------------------- If you want to find psilocybin in species that are not yet known to be psychoactive, you should do chemical tests. That way you won't get sick and die all the time. - Alan Rockefeller Reality is that which, when you stop believing in it, doesn't go away." - Philip K. Dick
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2FiNiTe
ConsideratlyKilling Me



Registered: 06/12/06
Posts: 1,635
Loc: New England
Last seen: 7 years, 5 months
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Re: Germination Experiment: findings may surprise u. [Re: PinheadX]
#7079600 - 06/22/07 12:34 PM (16 years, 8 months ago) |
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From the sounds of it the mycellium is particularly speedy, I'm sure if you were to inject into a BRF cake you'd get some quick colonization. I just started some LC myself but the honey I used left a bunch of debris inside. I'm kinda disappointed and I'm not sure if the spore will even germinate inside the solution? Any ideas?
-------------------- "Ours is a world of nuclear giants and ethical infants. We know more about war that we know about peace, more about killing that we know about living." General Omar N. Bradley
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PinheadX
Stranger thanyou



Registered: 04/25/07
Posts: 1,414
Loc: TX Gulf Coast
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Re: Germination Experiment: findings may surprise u. [Re: 2FiNiTe]
#7080392 - 06/22/07 04:12 PM (16 years, 8 months ago) |
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I really wouldn't worry about the sediment. It's probably just pollen, proteins from the honey, etc. If I recall correctly, honey is antibacterial for the most part, though it can carry Botulinin spores. If you PC it, I think you take care of that, so I really wouldn't sweat the sediment. Mine was filtered honey, but I would have used unfiltered if I had had it.
Use the LC. It'll be fine.
-------------------- If you want to find psilocybin in species that are not yet known to be psychoactive, you should do chemical tests. That way you won't get sick and die all the time. - Alan Rockefeller Reality is that which, when you stop believing in it, doesn't go away." - Philip K. Dick
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PinheadX
Stranger thanyou



Registered: 04/25/07
Posts: 1,414
Loc: TX Gulf Coast
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Re: Germination Experiment: findings may surprise u. [Re: PinheadX]
#7080401 - 06/22/07 04:14 PM (16 years, 8 months ago) |
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also, next time you make up the honey solution, you can filter it through a coffee filter into another jar and then PC it so you don't get the sediment. Just make sure you start with hot water, so the honey dissolves.
-------------------- If you want to find psilocybin in species that are not yet known to be psychoactive, you should do chemical tests. That way you won't get sick and die all the time. - Alan Rockefeller Reality is that which, when you stop believing in it, doesn't go away." - Philip K. Dick
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2FiNiTe
ConsideratlyKilling Me



Registered: 06/12/06
Posts: 1,635
Loc: New England
Last seen: 7 years, 5 months
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Re: Germination Experiment: findings may surprise u. [Re: PinheadX]
#7080710 - 06/22/07 05:44 PM (16 years, 8 months ago) |
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I did exactly that (PC). Thanx tho thats awesome I was starting to get worried, but I just checked them I'm starting to maybe see some growth...i think. Oh well thanx again. Great coffee filter idea.
-------------------- "Ours is a world of nuclear giants and ethical infants. We know more about war that we know about peace, more about killing that we know about living." General Omar N. Bradley
Edited by 2FiNiTe (06/22/07 05:45 PM)
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Grogan
Shroomieologist



Registered: 05/20/03
Posts: 1,146
Last seen: 8 years, 9 months
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Re: Germination Experiment: findings may surprise u. [Re: 2FiNiTe]
#7081230 - 06/22/07 07:50 PM (16 years, 8 months ago) |
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14 is about normal as far as I know.. also if something looks like its growing UBER fast.. its probaly a contam! I hate when contams do that crap.
-------------------- "Maybe a cow occasionally ate a shroom, but it certainly wouldn't be such a potent shroom that the cow would be trippin balls. " LOL
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2FiNiTe
ConsideratlyKilling Me



Registered: 06/12/06
Posts: 1,635
Loc: New England
Last seen: 7 years, 5 months
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Re: Germination Experiment: findings may surprise u. [Re: Grogan]
#7094462 - 06/26/07 11:36 AM (16 years, 7 months ago) |
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Defiantly not a contam. tho, I can tell you that with 99% certainty.
-------------------- "Ours is a world of nuclear giants and ethical infants. We know more about war that we know about peace, more about killing that we know about living." General Omar N. Bradley
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HippieChick
Chicks can do it too!



Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 3 years, 1 month
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Re: Germination Experiment: findings may surprise u. [Re: 2FiNiTe]
#7094589 - 06/26/07 12:10 PM (16 years, 7 months ago) |
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Quote:
2FiNiTe said: Dude where do u see tyvek mentioned anywhere in that quote?
and just so you see where im coming from about the light issue
http://www.shroomery.org/forums/showflat.php/Number/735753#735753 http://www.shroomery.org/forums/showflat.php/Number/734911#734911
And again I'm not saying that light is a huge factor I'm just showing you this is the general consensus among most people even tho it maybe an myco myth.
Dude , you're coming from 5 years in the past . You still use coir as a casing layer and not a sub ?
This is a science , and hence , things change .
Keep updated
Peace,Love,Happiness and Harmony Hippie Chick
-------------------- Peace,Love and Happiness HC Freedoms just another word for nothing left to lose.............. I LUV My Greenhouse http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848 My First Pans http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058
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2FiNiTe
ConsideratlyKilling Me



Registered: 06/12/06
Posts: 1,635
Loc: New England
Last seen: 7 years, 5 months
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Re: Germination Experiment: findings may surprise u. [Re: HippieChick]
#7094611 - 06/26/07 12:17 PM (16 years, 7 months ago) |
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Coir was in my substrate. I don't plan on casing them at all, from what you told me a sometime ago Coir substrates are not generally cased. Your Coir grow a few months ago is actually what spawned this experiment since I had starting adding Coir into my sub. I noticed the benefits.
And yeah I know those are outdated posts I was just giving him a separate point of view. As old and outdated as it is. But like I said at the beginning I realize I was under a common misconception about this subject. Light alone I now know cannot cause fruiting.
Edited by 2FiNiTe (06/26/07 12:20 PM)
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