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Offlineupupup
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Innoculating the same jar twice?
    #706212 - 06/27/02 07:15 PM (14 years, 5 months ago)

I have been reading some threads about birdseed quart jars. Several folks recommend not shaking the jars immediatly following innoculation. My questions are;

1. Why do jars fail to colonize if they are shaken after innoc.?
2. Can you re-innoculate?

Thanks for your help.


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Offlinebassplayer74
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Re: Innoculating the same jar twice? [Re: upupup]
    #706284 - 06/27/02 07:47 PM (14 years, 5 months ago)

1. They don't unless your sterile tek was bad or you did something else wrong. Shaking helps distribute the spores you just pu in there.

2. Yes....But if they didnt colonize the first time, why would they the second. Greater chance of contams too.


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InvisibleRoadkill
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Re: Innoculating the same jar twice? [Re: upupup]
    #706810 - 06/27/02 11:51 PM (14 years, 5 months ago)

you probably have a bacterial contam....and you just can't see it.

don't waste putting in more spores.

how many days has it been since you inoculated?

and how much spores did you use?


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OfflineAnnoA
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Re: Innoculating the same jar twice? [Re: upupup]
    #706820 - 06/27/02 11:55 PM (14 years, 5 months ago)

>Several folks recommend not shaking the jars immediatly following innoculation.

You SHOULD shake the jars immediately after the inoculation, but THEN wait with the shaking untill a decent amount of growth shows up.


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Offlineupupup
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Re: Innoculating the same jar twice? [Re: Anno]
    #707181 - 06/28/02 07:31 AM (14 years, 5 months ago)

Hmmm. OK. I did shake them up just a tad when first innoc. They are fine. I am just trying to clarify some things. No contams. Thanks everyone.

Damn Anno, you are everywhere.


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Amazon Shop for: ½ Pint Jars, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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