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Offlinekrypto2000
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Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 5 months
colonization too slow
    #7015569 - 06/06/07 01:07 PM (16 years, 9 months ago)

I've been growing mushrooms for about 6 months now, I started with the pf-tek and now I use wbs and case them with miracle grow soil mix. I'm currently working on my 3rd batch using the later method.

I figure now I will just work on improving my technique, my first problem is that the jars always seem to take a long time to colonize. I have taken some pictures to show my setup. The temperature is always around 83f never exceding 84 or dropping below 80.

The picture of these jars I just took which is 2 weeks and a day into the incubation process. The way I sealed the jars was by first putting on some aluminum foil over the jar, then putting on the lower lid, taping the holes on that, then putting a coffee filer on top of that and then screwing the top lid on. This has seemed to remain sterile so far. After pressure cooking them I just inject them as is and place them in the container. I normally only shake them once about a week to a week and a half into the colonization.

I was worried maybe the air holes are too small and that is what is holding them back however I was worried it may be alot easier for them to become contaminated otherwise.


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OfflineDiabloSmurf
Shake it.. Shakeit.. sugaree!
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Registered: 06/25/04
Posts: 1,177
Loc: Ohio
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Re: colonization too slow [Re: krypto2000]
    #7015585 - 06/06/07 01:14 PM (16 years, 9 months ago)

Use LC, it speeds up the process alot. You have to take into consideration the germinating time and things like that.. Everything you have looks awesome. and you're doing things right minus the aluminum foil under the lid?

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Offlinekrypto2000
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Registered: 12/05/06
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Re: colonization too slow [Re: DiabloSmurf]
    #7015596 - 06/06/07 01:17 PM (16 years, 9 months ago)

Yeah I don't know where I got the idea for the aluminum foil at, I had been doing it since the pf tek and carried it over I guess. I won't use it next time if it's unnecessary. What is LC? Sorry I'm not familiar with the term.

edit: Liquid Culture, nevermind :smile:

Edited by krypto2000 (06/06/07 01:19 PM)

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OfflineDiabloSmurf
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Registered: 06/25/04
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Re: colonization too slow [Re: krypto2000]
    #7015607 - 06/06/07 01:20 PM (16 years, 9 months ago)


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OfflineDiabloSmurf
Shake it.. Shakeit.. sugaree!
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Registered: 06/25/04
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Re: colonization too slow [Re: DiabloSmurf]
    #7015619 - 06/06/07 01:23 PM (16 years, 9 months ago)

haha gotcha

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Offlinekrypto2000
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Re: colonization too slow [Re: DiabloSmurf]
    #7015798 - 06/06/07 02:19 PM (16 years, 9 months ago)

Thanks for the link of the LC, I've got a quick question regarding the vacu-tainers I didn't quite understand.

Quote:

You can bore a small hole big enough for a syringe needle in the top of a jar. (Half pints work best for this) Now put a blob of silicone sealant on it (preferable transparent) on both sides. Swirl it around to make sure it is a centimeter thick around the hole on each side. This is a self healing inoculation point so you can add spores and suck up inoculant without having to open the jar after sterilization.




I think what that is saying is to take a 1/2 pint, screw the lid on tightly and then poke a single hole for the syringe needle to fit through and glue the hole shut before you pressure cook it right?

Do you just tear the glue off afterwards and inject the spores and it will be ok, or do I just make a big dab of silicone over the hole and poke strait through leaving it on there?

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OfflineDiabloSmurf
Shake it.. Shakeit.. sugaree!
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Registered: 06/25/04
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Last seen: 15 years, 9 months
Re: colonization too slow [Re: krypto2000]
    #7015814 - 06/06/07 02:22 PM (16 years, 9 months ago)

put the glue on and pressure cook it. the silicone will act as a self healing injecting port

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Offlinekrypto2000
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Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 5 months
Re: colonization too slow [Re: DiabloSmurf]
    #7037368 - 06/12/07 10:33 AM (16 years, 9 months ago)

ok, well I thought I should just bump this instead of making a new thread. Basically the jars still look the same now a week later, today will be the 3rd week exactly since inoculation and they have not really moved at all in the past week, I definitely think something is holding them back. If it is the lack of oxygen, which I would think is most likely, should I just make the holes bigger or what would be the best way to go about this?

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InvisibleRoadkillM
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Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: colonization too slow [Re: krypto2000]
    #7037386 - 06/12/07 10:38 AM (16 years, 9 months ago)

why do you have alum foil under the lid?


this is probably your problem...

or one of them!~


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.


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Offlinekrypto2000
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Registered: 12/05/06
Posts: 11,579
Last seen: 4 years, 5 months
Re: colonization too slow [Re: Roadkill]
    #7037655 - 06/12/07 11:45 AM (16 years, 9 months ago)

I really have no idea why I started to do that. I didn't even think of that before, but yeah I'm sure that would be alot better without it.

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OfflineDiabloSmurf
Shake it.. Shakeit.. sugaree!
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Registered: 06/25/04
Posts: 1,177
Loc: Ohio
Last seen: 15 years, 9 months
Re: colonization too slow [Re: krypto2000]
    #7037709 - 06/12/07 12:00 PM (16 years, 9 months ago)

yeah i dont think you can remove it now without risking contams.. But for future reference.. dont do it bro :smile:

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InvisibleRoadkillM
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Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: colonization too slow [Re: krypto2000]
    #7038103 - 06/12/07 01:34 PM (16 years, 9 months ago)

If you don't have a glovebox, still air box or a flowhood...

here is an idea for you....

get some microspore tape, a large see through plastic bag, and a can of Oust.

Take the plastic bag and spray the iside with Oust...

then put the jar inside the bag.

sterilize something with Isopropyl Alcohol to shove through and make the hole bigger in that lid...

then take the microspore tape and put that over the larger hole you made.


this will allow more gas exchange.

and maybe your jars will take off again...and fully colonize for you.


tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.


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OfflineMycodood
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Registered: 05/22/07
Posts: 277
Loc: USA
Last seen: 3 years, 1 month
Re: colonization too slow [Re: krypto2000]
    #7038113 - 06/12/07 01:37 PM (16 years, 9 months ago)

Yeah I think you got confused on this one.
The foil goes on last and comes off after pcing.

Best way to do this is:

- Take the cap and drill a hole drilled through for inoc and gasex,
I do a 3/8 or 5/8 hole
- Place it on the jar rubber side down, then tyvek over the cap,
then screw on the band over the tyvek.
- Put alum foil over the top to keep the tyvek dry during PC.

- After pcing and the jars cool take the foil off.
- Swab the top of the jar tyvek and all with alcohol and noc it.
- Cover the hole with micropore tape.

Had 2 jars contam on me out of over 100 so far and it was because
of open air g2g transfer.

I do this for rye and PF type jars and I skip the top verm layer
on the top of the PF jars.

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