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Invisibleblaine
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Wild mushroom preservation through cultivation: Possible?
    #7023338 - 06/08/07 04:01 PM (16 years, 9 months ago)

(I searched but could not find a thread for my question but I'm certain it exists somewhere.)

With perfect conditions recently, I noticed a small patch of mushrooms growing in my yard. An unusually busy schedule prevented me from even identifying them before they were gone. Oh well.

As mostly a lurker on the forums, it made me wonder what the best method of cultivating something from (not in) the wild. With sterile conditions being a requirement, I wondered what the process would be.

It seems that a sporeprint wouldn't be a clean enough source. Maybe try to disinfect/clean :eek: it and then (in a glovebox) clone it?

Apologies if this seems inane. Like I said: Maybe I'm using the wrong words to search for this answer.


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InvisibleBlutjager
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Re: Wild mushroom preservation through cultivation: Possible? [Re: blaine]
    #7023518 - 06/08/07 05:06 PM (16 years, 9 months ago)

It can be done,I'm not sure how one goes about doing it but I know it can be done an agar if you just keep sectoring of mycelium away from any contamination that is on the print

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OfflineDr_T
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Re: Wild mushroom preservation through cultivation: Possible? [Re: blaine]
    #7023525 - 06/08/07 05:08 PM (16 years, 9 months ago)

You would want to clone it from a section within the cap. Swab the cap with alcohol, split it in half, cut out a little section with a sterile knife (or a core with a hollow needle) and then use that on agar or LC.

Good to go.


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Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.

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Invisibleblaine
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Re: Wild mushroom preservation through cultivation: Possible? [Re: Dr_T]
    #7025792 - 06/09/07 08:41 AM (16 years, 9 months ago)

That's mostly what I imagined but didn't know if alcohol would be too damaging.

Blut: I'm actually somewhat surprised that it would be possible to successfully isolate mycelium from contamination. I've always thought that contams are 50% what you can see and 50% unseen and that, generally, "once contaminated, always contaminated" which was why a lot (most?) advice I've seen for contams during incubation say, "throw 'em out!" I guess my logic is merging cultivation with strain isolation, though.

Thanks for the info! :grin:


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OfflineRogerRabbitM
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Re: Wild mushroom preservation through cultivation: Possible? [Re: blaine]
    #7025804 - 06/09/07 08:45 AM (16 years, 9 months ago)

Most commercial edible strains have been cloned from wild fruitings. With agar, you can easily isolate the mushroom mycelium away from contaminants.
RR


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OfflineDr_T
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Re: Wild mushroom preservation through cultivation: Possible? [Re: blaine]
    #7025818 - 06/09/07 08:52 AM (16 years, 9 months ago)

Quote:

blaine said:
That's mostly what I imagined but didn't know if alcohol would be too damaging.
Thanks for the info! :grin:




Alcohol on the outside of the cap- then get a piece of flesh from inside. I know there's a tek somewhere that shows how to do this...


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Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.

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OfflineRogerRabbitM
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Re: Wild mushroom preservation through cultivation: Possible? [Re: Dr_T]
    #7025839 - 06/09/07 09:00 AM (16 years, 9 months ago)

Never use alcohol on a mushroom. Use iodine. Some growers use peroxide, but iodine is better. There better not be a tek saying to use alcohol on a mushroom. If there is, it's bogus and needs to be deleted. Alcohol is highly toxic to mushroom mycelium.

I usually just tear the stem lengthwise and take a core sample from the middle.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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OfflineDr_T
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Re: Wild mushroom preservation through cultivation: Possible? [Re: RogerRabbit]
    #7025996 - 06/09/07 09:58 AM (16 years, 9 months ago)

Quote:

RogerRabbit said:
Never use alcohol on a mushroom.  Use iodine. 




D'oh! Thanks RR. I knew I'd seen something about this, but I should have taken the time to do a search before posting advice. :blush:

But you think the basic idea is sound- cloning a wild mushroom using a tissue sample on agar or LC?

EDIT: I have access to Betadine (povidone/iodine) swabs- how do you feel about those?


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Roger Rabbit said: Growing mushrooms is part art, and part science, but it's not magic.

Edited by Dr_T (06/09/07 09:59 AM)

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OfflineRogerRabbitM
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Re: Wild mushroom preservation through cultivation: Possible? [Re: Dr_T]
    #7026110 - 06/09/07 10:40 AM (16 years, 9 months ago)

Agar, not lc. Agar is for cloning and isolation away from contaminants, because you have the flat, two dimensional plane of a petri dish to work with. You can't isolate anything in the three dimensional space of lc. In fact, I think lc's are a horrible way to expand tissue. They're far slower than grains and you have no idea if you have healthy mycelium or contaminants until you grow them out.

Betadine is fine.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Invisibleblaine
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Re: Wild mushroom preservation through cultivation: Possible? [Re: RogerRabbit]
    #7027758 - 06/09/07 08:42 PM (16 years, 9 months ago)

Quote:

RogerRabbit said:
Most commercial edible strains have been cloned from wild fruitings.  With agar, you can easily isolate the mushroom mycelium away from contaminants.




Right! That's what I thought was somewhat funny. Specific strain cultivation had to start with a wild specimen at some point! :smile:

It's encourgaing to hear it's not rocket science.

Everyone: thanks for taking the time to share some good info!


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OfflineRogerRabbitM
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Re: Wild mushroom preservation through cultivation: Possible? [Re: blaine]
    #7027895 - 06/09/07 09:17 PM (16 years, 9 months ago)

For more reading on mushrooms and contamination, check out this thread that I just put up in the gourmet and medicinal forum. http://www.shroomery.org/forums/showflat.php/Number/7027870#Post7027870
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Offlinefigurine
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Re: Wild mushroom preservation through cultivation: Possible? [Re: RogerRabbit]
    #7027950 - 06/09/07 09:36 PM (16 years, 9 months ago)

Quote:

RogerRabbit said:
Never use alcohol on a mushroom. Use iodine. Some growers use peroxide, but iodine is better. There better not be a tek saying to use alcohol on a mushroom. If there is, it's bogus and needs to be deleted. Alcohol is highly toxic to mushroom mycelium.

I usually just tear the stem lengthwise and take a core sample from the middle.
RR




This immediately came to mind after reading Dr_T's reply and your reply thereafter: http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5874305/an/0/page/0

Thanks for mentioning alcohol's effect; some dangerous criminal that I know is going to reconsider how he is going to make an lc later on.


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