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InvisibleGoodbyeOrb
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My "Damn, I've got green mold" Thread
    #6923658 - 05/16/07 07:26 AM (16 years, 8 months ago)

As you have probably guessed by the title of the thread and my recent posts, one of my WBS jars has met it's fungal match.  Anyway, thought I'd throw some pics up here for anyone who doesn't know what green mold looks like to check out.  I got some pretty good photos, so enjoy!  :thumbup:

These first four shots are just some pics of the actual mold, on WBS.








This is a shot showing the point of contamination, which, in this case, was the tyvek under the breather hole I drilled in the lid.  (Note:  This hole was drilled separate from the innoc hole for the purpose of gas exchange.)

Further examination revealed that a small piece of metal protruding from the hole punctued the tyvek filter, which of course defeats the  qualities of the tyvek.

Not really having anything better to do, I decided to plant them outside.  A shady spot under a bush was chosen.  In this photo, you see the contaminated jar, my location, and a bag of dehydrated manure & hummus.

Here you see the WBS spread evenly in the bottom of the shallow hole i dug.


And, finally, the finished product.  Who knows if it will work, but they were trash anyway, so we shall see.


Okay guys...  Just remember a few key things.

1)  Get contaminated jars away from your other jars, and  preferably out of your house.

2)  Check and double check EVERYTHING.  This contamn could have easily been avoided if I inspected my lids before beginning.  Instead, in a hurry to PC, I stacked my lids and drilled them all at once.

3)  Be patient.  (Refer to number 2)

4)  And, finally, although some people may tell you different, try to avoid sticking your nose in a jar full of contamns to see what it smells like. 

Have fun, good luck.  I hope I helped.


RR -  Feel free to hit me with some feedback, I'm curious how I am doing.  Thanks man.


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OfflineMeatfart
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Re: My "Damn, I've got green mold" Thread [Re: GoodbyeOrb]
    #6929788 - 05/17/07 11:25 AM (16 years, 8 months ago)

GoodbyeOrb said: Check and double check EVERYTHING. This contamn could have easily been avoided if I inspected my lids before beginning. Instead, in a hurry to PC, I stacked my lids and drilled them all at once.

Why would this leave you open for contams? Did you have a warped lid or something?


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InvisibleGoodbyeOrb
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Re: My "Damn, I've got green mold" Thread [Re: Meatfart]
    #6929855 - 05/17/07 11:45 AM (16 years, 8 months ago)

If you look closely at this picture, you can see where a piece of the metal shaving was left attached to the underside of the lid, and subsequently punctured the tyvek.



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OfflineRogerRabbitM
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Re: My "Damn, I've got green mold" Thread [Re: GoodbyeOrb]
    #6935936 - 05/18/07 07:00 PM (16 years, 8 months ago)

That looks like aspergillus, which is much more toxic than the more common trichoderma. You'll notice the colonies are circular in shape. That's one of the biggest telltale signs. Another is the serrated edges around the leading edge. The green is also a much lighter color, indicating Aspergillus contamination.

It's extremely important to keep your filter dry and free of grain 'juice' at all times. That's why I drill four holes in each lid, 1/16" in diameter. That sized hole is smaller than the kernel size, so when I shake the jars, no grains ever come in contact with the filter. Some growers also leave the foil on, which causes the filter to get wet with condensation. This also leads to contamination, so be sure to remove the foil as soon as the jars are removed from the pressure cooker. Your wbs in that picture is way too wet, so that's probably how your filter got wet, which then allowed it to colonize right through with the mold. I don't see a hole in the filter, but that's also a possibility, but it's much more likely a wet filter caused it or the filter itself wouldn't have grown the mold.

I drill holes through ten jar lids at once, but the difference is a much smaller drill bit, and I make sure it's sharp, and don't press down too hard. That way, no burrs are created.
RR


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InvisibleGoodbyeOrb
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Re: My "Damn, I've got green mold" Thread [Re: RogerRabbit]
    #6936026 - 05/18/07 07:28 PM (16 years, 8 months ago)

Yeah i had a couple of silly mistakes on this, as it's my first WBS. First, my tyvek filter was UNDER the lid, inside the jar. Second, my WBS obviously is too wet. Fun times. The others i innoced took off, only to stall shortly after. (pic below) I've got some serious rhizo in one of the jars though, so i am contemplating ways to birth the rhizo growth before it contams, as they aren't contamned yet, and plant outside as i did with the contaminated jar in the pics. Any thoughts?



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Re: My "Damn, I've got green mold" Thread [Re: GoodbyeOrb]
    #6936034 - 05/18/07 07:31 PM (16 years, 8 months ago)

One more question, what are some good signs that my WBS has the right moisture content next go around? I was thinking about saoking, then simmering, draining, like i did last time, but i was contemplating spreading the WBS onto paper towels to dry the outside of the seeds this time, as i read a thread where the poster said that the moisture should all be inside the seeds, and, when shaked, the WBS should have the consistency of sand. Any thoughts? What about that bird seed? is it okay, or should I switch to a straight millet? Thanks RR.


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OfflineRogerRabbitM
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Re: My "Damn, I've got green mold" Thread [Re: GoodbyeOrb]
    #6936059 - 05/18/07 07:40 PM (16 years, 8 months ago)

Search posts under my name for 'rye tek'. You can use it for wbs too. You can also look at the short, low resolution sample on my website of the grain prep tek. It has a short video of what the grains should look like when ready to load. They'll look and feel dry.
RR


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semper in excretia sumus solim profundum variat

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Re: My "Damn, I've got green mold" Thread [Re: RogerRabbit]
    #6936064 - 05/18/07 07:43 PM (16 years, 8 months ago)

You can change the name to I've got Aspergillus if you want, that way people don't get confused by my ignorance.

In your personal experience, should I ditch the WBS and go with the rye? If so, do you have a personal favorite?


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Re: My "Damn, I've got green mold" Thread [Re: GoodbyeOrb]
    #6936104 - 05/18/07 07:56 PM (16 years, 8 months ago)

BTW I can't find "rye tek" in any of your main threads, are you referring to the many threads in which you weighed in on other people? Thanks.


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My rye tek [Re: GoodbyeOrb] * 1
    #6936247 - 05/18/07 08:49 PM (16 years, 8 months ago)

My rye tek:
Measure out your organic rye berries from a health food store, one cup for each quart jar you intend to make. Place them in a large pot. Rinse the heck out of them. Fill the pot with hot tap water, shake and swirl it around and pour it out. Do this three or four times until the water you pour out is clear. You'll be able to see when you have nice clean water to pour off instead of water filled with chaff and dirt.

You want to now cover the rye berries with three times as much hot tap water as you have rye. Use half coffee and half plain water. In other words, if you have two inches of rye in the bottom of your pan, you should have six inches of water/coffee above that, for a total of 8 inches.  Add approximately a 1/4 teaspoon of gypsum per cup of rye. Stir these into the water/grain well. For my large kettle with ten cups of rye and two to three gallons of water, or (coffee/water)I add a tablespoon of gypsum and mix in. Cover and leave this to sit for 6-24 hours.

Stir well and set the pot on the stove. Bring to a boil. Boil for ten minutes, then, WHILE BOILING, drain the contents through a very large colander. (spaghetti strainer) If you're making a large batch, you may need more than one colander. Tip the colander side to side to get the rye to drain as much of the water as you can. Then, shake the colander in order to 'toss' the grain. This will cause a lot of steam to rise from your rye. Great. Do this a time or two, then let it sit for five minutes, then repeat. When all the moisture that will drip or evaporate from your rye has already done so, load your jars. The rye should look and feel dry to the touch when you load the jars. All the moisture you need is inside the grain.

Fill jars no more than 2/3 full if they are to receive grain to grain transfers, or no more than 3/4 full if they are to be inoculated by spore syringe or agar wedge. Use a lid with a synthetic filter disk, polyfill, tyvek or similar. Cover with foil and PC the jars for at least 90 minutes at 15lbs. I use 120 minutes. When the jars are cool, they're ready to inoculate.

This is the procedure that's demonstrated on my DVD.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Re: My rye tek [Re: RogerRabbit]
    #6942881 - 05/20/07 01:47 PM (16 years, 8 months ago)

Hey are your DVD's up for sale yet? Ty.


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OfflineRogerRabbitM
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Re: My rye tek [Re: GoodbyeOrb]
    #6943596 - 05/20/07 05:21 PM (16 years, 8 months ago)

They're supposed to get shipped to me Monday by second day air. If I get them Wednesday, I'll unlock my storefront and open it up as soon as I check them out and they're all good. I'll make the announcement in the forum and my banner will go up at that time.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Re: My rye tek [Re: RogerRabbit]
    #6943653 - 05/20/07 05:39 PM (16 years, 8 months ago)

Are you pre-selling at all to shroomerites? Also, what is the point of the half & half coffee/water mix?


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Re: My rye tek [Re: RogerRabbit]
    #6946302 - 05/21/07 09:05 AM (16 years, 8 months ago)

RR - Also, do you just innoc through one of the four 1/16 holes you mentioned? I think my syringes (courtesy of sporeworks) might be a little larger than that. Also, do you cover your innoc holes with microspore or anything after innoc?


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Re: My rye tek [Re: GoodbyeOrb]
    #6946987 - 05/21/07 12:07 PM (16 years, 8 months ago)

I'm not pre-selling the videos. I don't want to take anyone's money until I can offer same day shipping. The manufacturer just called and said the videos were shipped today, Monday, by 2'nd day air, so I should have them Wednesday. As soon as I open the box, plop one into the DVD player and see it work, I'll unlock my storefront and take orders. I have everything set up, just waiting for the disks to arrive.

I inoculate in front of a flow hood, by actuall lifting the lid up and dropping in an agar wedge or by g2g. If your injecting without a flowhood, look in to agar's injection port lids. http://www.natures-resources.com/
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Offlinefirefairy323
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Re: My rye tek [Re: RogerRabbit]
    #15697298 - 01/21/12 12:37 PM (12 years, 9 days ago)

i made a mistake. i rinsed my berries really well. then covered with the coffee/gypsum water solution and brought to a boil for ten minutes immediately, without letting them sit for 6-24 hours. should i toss this and start over?


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Re: My rye tek [Re: RogerRabbit]
    #27218861 - 02/21/21 05:10 PM (2 years, 10 months ago)

Hey :smile:

Is it possible to use this technique with unicorn bags to do more bulk?  Say 4 cups of rye per bag? That way I could do 1 bag per tub.  Also, how important is using the coffee with water?  What is it for? 

Thank you in advance


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Re: My rye tek [Re: sacerdoti]
    #27218916 - 02/21/21 05:37 PM (2 years, 10 months ago)

This is a very old thread.

Yes, you can run bags with any grain. 1 bag per tub is standard. Don't use coffee.
Click the more inoculation points for less in my signature, also check out nates thread on bags.


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