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pinky2totters
Stranger
Registered: 04/27/07
Posts: 159
Last seen: 14 years, 10 months
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Re: But how will there be gas exchange? [Re: _OttO_]
#6924297 - 05/16/07 10:15 AM (16 years, 10 months ago) |
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I think I'm going to go get some tyvek...
I think. Argh, willsomeone tell me what to dO!
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_OttO_
Over Stimulated
Registered: 06/01/05
Posts: 2,588
Loc: Up Over
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Re: But how will there be gas exchange? [Re: pinky2totters]
#6924307 - 05/16/07 10:17 AM (16 years, 10 months ago) |
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Punch a hole in your lids with a screw driver - or use a drill if you want to be neat, then wad a ball of polyfill into this hole nice and tight.
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_OttO_
Over Stimulated
Registered: 06/01/05
Posts: 2,588
Loc: Up Over
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Re: But how will there be gas exchange? [Re: blood4blood]
#6924312 - 05/16/07 10:19 AM (16 years, 10 months ago) |
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Quote:
blood4blood said: gas exchange is what you need during incubation! so your doing brf/verm jars? if you have the dry verm layer on top you should have 4 1/4" holes poked in the lids (i use a nail) not covered with anything to allow for proper gas exchange.
This is also very good advice...
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pinky2totters
Stranger
Registered: 04/27/07
Posts: 159
Last seen: 14 years, 10 months
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Re: But how will there be gas exchange? [Re: pinky2totters]
#6924325 - 05/16/07 10:22 AM (16 years, 10 months ago) |
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http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6345298
I think this answers my questions. To the post office!
Sorry for not finding it earlier, I did search...
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GoodbyeOrb
Self-SacrificingPotency Tester
Registered: 04/09/07
Posts: 5,182
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Re: But how will there be gas exchange? [Re: pinky2totters]
#6924350 - 05/16/07 10:28 AM (16 years, 10 months ago) |
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THOSE ARE FOR WILD BIRD SEED JARS. You do NOT need polyfill or tyvek or ANYTHING OTHER THAN vermic and holes for PF Cakes. Is everyone here deaf or something?
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blood4blood
Calmer Than You Are
Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: But how will there be gas exchange? [Re: GoodbyeOrb]
#6924371 - 05/16/07 10:42 AM (16 years, 10 months ago) |
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Quote:
GoodbyeOrb said: THOSE ARE FOR WILD BIRD SEED JARS. You do NOT need polyfill or tyvek or ANYTHING OTHER THAN vermic and holes for PF Cakes. Is everyone here deaf or something?
i agree w/ you 100%
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dog
straw dog
Registered: 06/20/02
Posts: 2,790
Loc: Route 66
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Re: But how will there be gas exchange? [Re: GoodbyeOrb]
#6924586 - 05/16/07 11:29 AM (16 years, 10 months ago) |
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Quote:
THOSE ARE FOR WILD BIRD SEED JARS. You do NOT need polyfill or tyvek or ANYTHING OTHER THAN vermic and holes for PF Cakes.
Correct.
Quote:
I think. Argh, willsomeone tell me what to dO!
Sure. Go here and follow the instructions.
Good luck!
-------------------- Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.
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pinky2totters
Stranger
Registered: 04/27/07
Posts: 159
Last seen: 14 years, 10 months
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Re: But how will there be gas exchange? [Re: dog]
#6924957 - 05/16/07 12:46 PM (16 years, 10 months ago) |
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I'm back where I started, which is good. My original plan was to do regualr pf inoculation, and then when I posted making sure I was doing the right thing I got a whole bunch of replies about how I was wrong, and I wasn't going to have gas exchange and how I would get contamination. So I changed my idea and posted again, and again got a bunch of people telling me different things. The two layers of foil should work fine for me, I'm not really sure why someone didn't say "Yep" to me in the first place. I hope I won't have to start over (I just sterlized my first batch), but I think that I'll be alright. Thanks you those who told me the right thing to do, whether or not it confused me more.
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: But how will there be gas exchange? [Re: pinky2totters]
#6925151 - 05/16/07 01:35 PM (16 years, 10 months ago) |
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You have to remember that lots of new growers want to give advice to help, and I'm sure they have the best of intentions, but often they're DEAD WRONG on the advice they give, so always average out the answers you get until someone trusted comes along. There are some horrible answers above in this thread that are just flat out wrong.
Colonizing mycelium in jars REQUIRES gas exchange. Two layers of foil are no different than one layer of foil. However, the foil is for the purpose of protecting the openings in the LID during sterilization from the water that drips down off the top of the kettle or pressure cooker. With pf type cakes, there should be 4 holes in the lid large enough for a needle to get through. These are your inoculation holes, and serve later as your GAS exchange holes. People who try to colonize jars without gas exchange end up with bad cases of wet spot bacteria and other contaminants, or at best they end up with stalled jars.
You want to put 1/2"(1cm) of dry vermiculite in the top of your jars, and then put the lid with four small holes drilled around the edges. You then screw the ring on, and THEN cover with foil. The use of micro pore tape over the inoculation holes is optional. After sterilization, REMOVE the foil and inoculate through the holes, which from that time on are your gas exchange holes. Don't block them off. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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GoodbyeOrb
Self-SacrificingPotency Tester
Registered: 04/09/07
Posts: 5,182
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Re: But how will there be gas exchange? [Re: RogerRabbit]
#6925158 - 05/16/07 01:37 PM (16 years, 10 months ago) |
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Well, at least I wasn't too far off...
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pinky2totters
Stranger
Registered: 04/27/07
Posts: 159
Last seen: 14 years, 10 months
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Re: But how will there be gas exchange? [Re: pinky2totters]
#6925162 - 05/16/07 01:37 PM (16 years, 10 months ago) |
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I've done a bit more research at it seems the original PFTech colinization method is pretty slow because of the lack of gas exchange. Even with a verm layer, as many have said, the air wasn't getting in and colonization slowed. One way to fix this is to open up your jar in front of a fume hood,which I don't have. Another way was to add a polyfill filter (I assume tyvek is near equivalent).
So, for a number of reasons, half of my pf jars have a tyvek filter and half are using just aluminum foil. We'll see which has better results.
I'm sure someone has already tried this and is thinking "I already know what happens" but alas, I'm thoroughly confused at this point and need to know for myself.
As long as I get some colonization going for my first attempt I'll be very happy, I've purchased evereything I need and resterlizing jars with new substrate will cost me about six dollars. So, yeah, I'm happy.
Edit: I wrote this before I saw roger rabbit's post, which, to be honest, I was hoping for in the back of my mind. Anyway Thanks. This is basically what I did for some of my jars. I may have to do some over again in case water leaked in but it looks like I didn't get much dripage into the verm, and everything is dry. I'm going to open and dump one of my jars just to make sure, because I'm sure failure to check this will end in contamination. I think I have it all figured out... I hope. (This is really fun though, it's like high school science but it doesn't suck).
I didn't mean to make anyone feel bad, at a compeltely different site and forum for a different subject I'm a respected poster and often have to correct those who think they know what they're doing. No harm done, I'm here to learn about mycology at the end of the day that's what I'm doing.
Edited by pinky2totters (05/16/07 01:42 PM)
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pinky2totters
Stranger
Registered: 04/27/07
Posts: 159
Last seen: 14 years, 10 months
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Re: But how will there be gas exchange? [Re: GoodbyeOrb]
#6925189 - 05/16/07 01:44 PM (16 years, 10 months ago) |
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Quote:
GoodbyeOrb said: Well, at least I wasn't too far off...
I know you meant well.
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blood4blood
Calmer Than You Are
Registered: 04/25/07
Posts: 6,029
Loc: The Valley
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Re: But how will there be gas exchange? [Re: pinky2totters]
#6925204 - 05/16/07 01:50 PM (16 years, 10 months ago) |
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everyone meant well. like RR said everyone is trying to help everyone out and sometimes misinformation is dealt. that is something that have have to syphon through and wait till a more expierenced grower comes through and puts his advice in.
i hope you got it all figured out though. good luck man
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GoodbyeOrb
Self-SacrificingPotency Tester
Registered: 04/09/07
Posts: 5,182
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Re: But how will there be gas exchange? [Re: pinky2totters]
#6925209 - 05/16/07 01:51 PM (16 years, 10 months ago) |
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Well, what I meant was that I was one of the ones that was closer than some of the other suggestions in this thread.
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pinky2totters
Stranger
Registered: 04/27/07
Posts: 159
Last seen: 14 years, 10 months
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Re: But how will there be gas exchange? [Re: GoodbyeOrb]
#6925352 - 05/16/07 02:27 PM (16 years, 10 months ago) |
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Quote:
GoodbyeOrb said: Well, what I meant was that I was one of the ones that was closer than some of the other suggestions in this thread.
Okay, I'll rephrase. I know you were just trying to help. In fact, you did help! Thanks!
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GoodbyeOrb
Self-SacrificingPotency Tester
Registered: 04/09/07
Posts: 5,182
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Re: But how will there be gas exchange? [Re: pinky2totters]
#6925367 - 05/16/07 02:30 PM (16 years, 10 months ago) |
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lol, goodluck man.
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BlehMaestro
Stranger
Registered: 03/02/07
Posts: 754
Last seen: 11 years, 11 months
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Re: But how will there be gas exchange? [Re: pinky2totters]
#6925408 - 05/16/07 02:40 PM (16 years, 10 months ago) |
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Quote:
pinky2totters said: I've done a bit more research at it seems the original PFTech colinization method is pretty slow because of the lack of gas exchange. Even with a verm layer, as many have said, the air wasn't getting in and colonization slowed. One way to fix this is to open up your jar in front of a fume hood,which I don't have. Another way was to add a polyfill filter (I assume tyvek is near equivalent).
So, for a number of reasons, half of my pf jars have a tyvek filter and half are using just aluminum foil. We'll see which has better results.
I'm sure someone has already tried this and is thinking "I already know what happens" but alas, I'm thoroughly confused at this point and need to know for myself.
As long as I get some colonization going for my first attempt I'll be very happy, I've purchased evereything I need and resterlizing jars with new substrate will cost me about six dollars. So, yeah, I'm happy.
Edit: I wrote this before I saw roger rabbit's post, which, to be honest, I was hoping for in the back of my mind. Anyway Thanks. This is basically what I did for some of my jars. I may have to do some over again in case water leaked in but it looks like I didn't get much dripage into the verm, and everything is dry. I'm going to open and dump one of my jars just to make sure, because I'm sure failure to check this will end in contamination. I think I have it all figured out... I hope. (This is really fun though, it's like high school science but it doesn't suck).
I didn't mean to make anyone feel bad, at a compeltely different site and forum for a different subject I'm a respected poster and often have to correct those who think they know what they're doing. No harm done, I'm here to learn about mycology at the end of the day that's what I'm doing.
Take the foil off all the jars...no need to test which ones are gonna do better.
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ApJunkie
part-time Ninja
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Re: But how will there be gas exchange? [Re: BlehMaestro]
#6925705 - 05/16/07 04:04 PM (16 years, 10 months ago) |
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Sorry everyone, I must have been heavily mistaken. I've never conciously thought about gas exchange for colonization because I was similarly misinformed in the beginning, and must have lucked out. Thanks for the correction!
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pinky2totters
Stranger
Registered: 04/27/07
Posts: 159
Last seen: 14 years, 10 months
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Re: But how will there be gas exchange? [Re: BlehMaestro]
#6930798 - 05/17/07 04:01 PM (16 years, 10 months ago) |
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Thanks, it's all incubating. I think I've done it properly. My house is dusty so I have dust covers on em'. They are incubating at a nice even 80 thanks to my reptotherm. I'm 24 hours in it. I hope to see so mycelium soon, though I know I probably have a week to wait. Stay tuned for a grow log.
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