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Amazon Shop for: ½ Pint Jars, Pressure Cooker, Rye Grain, Vermiculite

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Offlinekykeon
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Just curious...
    #689230 - 06/19/02 10:30 PM (14 years, 5 months ago)

Hello all.

This question isnt of much importance, but its my first time
cultivating and i am full of questions...

strain: p. cubensis equador
media: rye on jars
temperature on storage closet: 27 C

the innoculation happened 3 days ago. now inside the jars, there are
at some places a milky white fluid surrounding some of the grains. when
i shaked them yesterday it dissapeared. today it showed up again in
larger amount i think. i have the feeling that everything is ok.
the question is: this white milky fluid is irrelevant of the mycelium, or is
it the first stage? i know its toooo early but i am just curious if this fluid
is of rye or of the mycelium.

thanx

peace all

kykeon
athens, greece


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The living ghost of Kykeon


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OfflineAnnoA
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Re: Just curious... [Re: kykeon]
    #689237 - 06/19/02 10:34 PM (14 years, 5 months ago)

Milky white fluid sounds very like bacterial contamination....:(

But you will se it, if the mycelium doesn?t grow, then it?s contamination, and then you?ll have to start anew, sorry.


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Offlinekykeon
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Re: Just curious... [Re: Anno]
    #689563 - 06/20/02 04:43 AM (14 years, 5 months ago)

Anno u r probably right, it started smelling...

but i am curious... if you boil rye or anything in a jar and let it in
a closet without FIRMLY closing the lid, there is absolutely no way
that the rye will be ok. If it could be ok, there wouldnt have
been inventions like refridgerators to keep food safe.

All food go bad outside the fridge.

So what is the secret here?

Kykeon
athens, greece


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Offlinehappygrins
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Re: Just curious... [Re: kykeon]
    #689569 - 06/20/02 04:46 AM (14 years, 5 months ago)

You have to pressure cook Rye.. did you?.. if not, then that is probably problem #1....

~~ happygrins


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Offlinekykeon
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Re: Just curious... [Re: happygrins]
    #689595 - 06/20/02 05:09 AM (14 years, 5 months ago)

sorry 4 not explaining that i was cautious ok:

i sterilized rye on pressure cook for 1 1/2 hours.
i worked with medical gloves and medical mask
dust free enviroment
cleared all table with betadine solution and alcohol.
used only sterilized water, knife etc.



well the problem could be:

a) i flamed the needle but i didnt waited long enough so as to make it red.
b) i pressure cooked the jars on sunday but i innoculated them on Monday night. That was a huge mistake i think... well?
c) i should work inside a cardbox cleaned with dettol [lysol].

well, i am waiting 4 opinions...

THANX 4 YOUR HELP GUYZ!!!!!!!!!!!

kykeon
athens, greece


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Edited by kykeon (06/20/02 05:19 AM)


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Invisiblerepobob
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Registered: 12/19/01
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Re: Just curious... [Re: kykeon]
    #689712 - 06/20/02 06:44 AM (14 years, 5 months ago)

Sounds like you are taking even more precautions than necessary. You should be OK. The only problem is that you are allowing your jars to sit in the closet with the lids lose. I would put them in some sort of incubator and leave the lids tight. You can use Polyfil or Tyvek or MM Filter Disks, but you do need some sort of filter when working with grains. Don't worry about leaving your jars a few days, I have left them for weeks and they were still OK. In fact PF suggests that you leave them a few days to see if contams grow.

Peace,
Bob


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OfflineMeneerCactus
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Re: Just curious... [Re: kykeon]
    #689717 - 06/20/02 06:47 AM (14 years, 5 months ago)

High Wykeon

Was there draft while making the syringe. I mean air flow in the room???
I discovered some milky stuff to today.
But my jar is 80% colonised and the milky looking moist is in the last very moisty place. I notice that mycelium contious to grow.
Something similar but different.

A refrigirator is also quite full of organism, don't think because it is cool it is sterile

I hope your jars start growing soon, or that you make a few new jars allready.

Goodluck and Peace.....

Eric


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"Millions of years of evolution just to .... light up a joint"


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Offlinekykeon
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Re: Just curious... [Re: repobob]
    #691448 - 06/20/02 09:16 PM (14 years, 5 months ago)

My dear e-friends,

Last night i throwed the contaminated contain of my jars.

I had to wear a mask, but still, there were 2 times that i was ready to vomit. Blah... its the worst smell ever... Funny, but i still can smell it. Hope i will not die

Anyway, i am very happy that the problem was found. Leaving the jars with lids loose is ofcourse a stupid thing to do. But i have read on a method at mushmush.nl that the lids are loose on pressure cooker and the lids are tighten only to be shaken then again loose.

Meneercactus told me to use polyfill in a hole in the lid. That means it can breathe thru polyfill but still is closed to contaminants. He is a dutch but i am not [ if u aint dutch, u aint much! ] and i didnt quite understood that. Thought that polyfill was only for innoculation.

So, I am starting again today. Thanks to all of you who replied my questions, u were extremely helpful. I was upset not for the contamination, but because i didnt know what to change in my method. Language problems, u see

PEACE

Kykeon
athens, greece


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Offlinekykeon
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Re: Just curious... [Re: MeneerCactus]
    #691454 - 06/20/02 09:19 PM (14 years, 5 months ago)

This is wyckeon.

The previous post with thanks also apply to you.
I mentioned the fridge because i think it is normal to have
contaminants if you leave food outside the fridge for days.

But if you keep the lid closed...

I AM MAKING NEW JARS TODAY!

I will keep u informed!

Wyckeon
Athens, Greece


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Offlinekykeon
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Registered: 05/30/02
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Last seen: 10 years, 2 months
Re: Just curious... [Re: Anno]
    #691612 - 06/20/02 11:46 PM (14 years, 5 months ago)


First of all, i want to laugh with joy, for after weeks of searching
i found VERMICULITE in greece!!!!!!!!!!!!

Anno, i think u r the man 4 this question:
they told me they have vermiculite in 3 sizes. small, medium, large.

Well? I think i will buy the medium sized. Its more logical if i dont know.

Thanx!!

kykeon
athens, greece



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Amazon Shop for: ½ Pint Jars, Pressure Cooker, Rye Grain, Vermiculite

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