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OfflineTheCaptain
addict

Registered: 09/04/01
Posts: 426
Loc: Canada
Last seen: 16 years, 4 months
Contamination Problems- help needed. *DELETED*
    #686273 - 06/18/02 03:07 PM (19 years, 7 months ago)

Post deleted by TheCaptain


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"I mean, it's real hard to be free when you are bought and sold in the marketplace. 'Course, don't ever tell anybody that they're not free 'cause then they're gonna get real busy killin' and maimin' to prove to you that they are."


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Invisibleluvdemshrooms
Two inch dick..but it spins!?
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Registered: 11/29/01
Posts: 34,246
Loc: Lost In Space
Re: Contamination Problems- help needed. [Re: TheCaptain]
    #686380 - 06/18/02 04:23 PM (19 years, 7 months ago)

Oh crap!!! Did they shut down the contamination forum?


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You cannot legislate the poor into prosperity by legislating the wealthy out of prosperity. What one person receives without working for another person must work for without receiving. The government cannot give to anybody anything that the government does not first take from somebody else. When half of the people get the idea that they do not have to work because the other half is going to take care of them and when the other half gets the idea that it does no good to work because somebody else is going to get what they work for that my dear friend is the beginning of the end of any nation. You cannot multiply wealth by dividing it. ~ Adrian Rogers


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InvisibleWe_come_in_peace
old hand

Registered: 05/14/02
Posts: 762
Loc: Alpha Centauri
Re: Contamination Problems- help needed. [Re: luvdemshrooms]
    #686441 - 06/18/02 05:05 PM (19 years, 7 months ago)

lol thats too funny.


I let my jars cool, wih lids loose, wrapped in a cloth thats been soaked in a water and bleach solution. This way the vacuum effect caused by your hot jars sucks in air thats steril while they cool. No contams since doing this.


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OfflineTheCaptain
addict

Registered: 09/04/01
Posts: 426
Loc: Canada
Last seen: 16 years, 4 months
Re: Contamination Problems- help needed. [Re: luvdemshrooms]
    #688190 - 06/19/02 01:18 PM (19 years, 7 months ago)

No, of course not smart ass. Last time i posted a problem in the contam forum it went unanswered for two weeks. Thats why i put it here.


--------------------
"I mean, it's real hard to be free when you are bought and sold in the marketplace. 'Course, don't ever tell anybody that they're not free 'cause then they're gonna get real busy killin' and maimin' to prove to you that they are."


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OfflineLilMerlin
Life magicapprentice
Registered: 03/19/02
Posts: 151
Loc: Europe.
Last seen: 18 years, 5 months
Re: Contamination Problems- help needed. [Re: TheCaptain]
    #688226 - 06/19/02 01:55 PM (19 years, 7 months ago)

Did you sterilise the surroundings with an alcohol-spray?

Did you sterilise your needle with fire or alcohol? (I personnaly don't like fire!)

What about your hands? to be sterilized as well or gloved and sterilized with alcohol.

What about your breath? Do not blow air. You can get masks...

If the contam comes from air (which we cannot know) you can try the "oven tek" in order to be sure. Before you put them in your incubator, slightly spray the jars and in the incubator as well.

One cause of contaminants could be the rye itself. I have read that some people pre-soak the rye for 20 hours before pressure-cooking so that water penetrates the grain and allows to kill the germs that are more resistant to heat when dry.

On three Maza jars I innoculated (with syringe) only one started, and late. Also, it was slow in spreading its mycelium. The grain transfer, on the opposite, is much faster.

I think people reported contam problems (but rare) with the filters you use. I use polyfill in a 6-7 milimeter hole in the lid.

Can't help more. (I used all of what I'm advising you, and also tried less secure settings and never had contams up to now, but I really spray a lot in the air with my alcohol spray... it may help.)

LilMerliln, still a newbie though.


--------------------
"Five grams in silent darkness." T. McKenna
Why take more? Take different!


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InvisibleRipple
Ripple
Male User Gallery

Folding@home Statistics
Registered: 05/16/02
Posts: 21,014
Loc: the timbers of Fennario
Trusted Cultivator
Re: Contamination Problems- help needed. [Re: TheCaptain]
    #688271 - 06/19/02 02:35 PM (19 years, 7 months ago)

Yup no one goes there


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The bus came by and I got on that's when it all began!



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Invisibleluvdemshrooms
Two inch dick..but it spins!?
 User Gallery


Registered: 11/29/01
Posts: 34,246
Loc: Lost In Space
Re: Contamination Problems- help needed. [Re: TheCaptain]
    #688487 - 06/19/02 04:33 PM (19 years, 7 months ago)

In reply to:

No, of course not smart ass.




Oh, you're such a big meanie!


--------------------
You cannot legislate the poor into prosperity by legislating the wealthy out of prosperity. What one person receives without working for another person must work for without receiving. The government cannot give to anybody anything that the government does not first take from somebody else. When half of the people get the idea that they do not have to work because the other half is going to take care of them and when the other half gets the idea that it does no good to work because somebody else is going to get what they work for that my dear friend is the beginning of the end of any nation. You cannot multiply wealth by dividing it. ~ Adrian Rogers


Extras: Filter Print Post Remind Me! Notify Moderator Top
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