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Offlinekykeon
Dead wishes

Registered: 05/30/02
Posts: 1,506
Loc: A universe right next to ...
Last seen: 17 years, 8 months
Innoculating thought...
    #685070 - 06/18/02 02:13 AM (22 years, 3 days ago)

Hello and Peace to all.

Today is a happy day. Last night I innoculated my first two jars of Equador
cubensis. I was more than cautious about sterilization and worked with gloves
and medicine mask... Hope everything will be ok.

The question is: After putting the spores to the syringe + water [i thought that
the water in there will be obviously colored, but nope] its time for innoculation.
I was holding the syringe upside down so as to innoculate and i saw that spores
[or should i say clumps of spores??] are heavier than water, so most of them
were at the bottom of the syringe. That means that logically, the first three-four
innoculations will be somehow stronger than the eighth or tenth...

Am I wrong? Perhaps its something wrong in my method...

Peace

Kykeon
athens, greece


--------------------
The living ghost of Kykeon

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OfflineAnnoA
Experimenter
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Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 26 days, 17 hours
Re: Innoculating thought... [Re: kykeon]
    #685074 - 06/18/02 02:20 AM (22 years, 3 days ago)

The spores are very small. Thus they don?t sink very fast. Means if you shake the syringe befor the inoculation, all the jars will receive roughly the same amount of spores.

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OfflineDERRAYLD
Constructus
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Registered: 05/13/02
Posts: 10,427
Loc: South Africa
Last seen: 12 minutes, 55 seconds
Trusted Cultivator
Re: Innoculating thought... [Re: kykeon]
    #685076 - 06/18/02 02:22 AM (22 years, 3 days ago)

When you flame sterilise the needle just before innoculation, suck in some of the flame air.
That way you`ll be able to shake the syringe to get those spores spread.

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Offlinekykeon
Dead wishes

Registered: 05/30/02
Posts: 1,506
Loc: A universe right next to ...
Last seen: 17 years, 8 months
Re: Innoculating thought... [Re: DERRAYLD]
    #685140 - 06/18/02 04:00 AM (22 years, 3 days ago)

Thanx Anno + Derrayld for the answer...

kykeon
athens, greece


--------------------
The living ghost of Kykeon

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Offlinefireworks_godS
Sexy.Butt.McDanger
Male

Registered: 03/12/02
Posts: 24,855
Loc: Pandurn
Last seen: 1 year, 4 months
Re: Innoculating thought... [Re: kykeon]
    #685159 - 06/18/02 04:17 AM (22 years, 3 days ago)

Personally, I had to shake my Mazatapec syringe for quite some time to get even distribution, because all the spores were in one giant clump when I received it in the mail. Maybe I had a profoundely rough syringe though. I definitely had enough for my last five jars, though!


--------------------
:redpanda:
If I should die this very moment
I wouldn't fear
For I've never known completeness
Like being here
Wrapped in the warmth of you
Loving every breath of you

:heartpump: :bunnyhug: :yinyang:

:yinyang: :levitate: :earth: :levitate: :yinyang:

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