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Hafrican
Mega-ultrachicken

Registered: 01/31/03
Posts: 105
Loc: SW Azerbaijan
Last seen: 16 years, 7 months
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"Proper" nomenclature for labeling agar plates/strains
#6834247 - 04/25/07 08:30 PM (16 years, 9 months ago) |
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Is there a proper method of labeling and denoting agar plates and different generations/transfers of tissue? Stammets and other texts make no mention of how to track and sort the genetic information.
Right now I'm working with a jury rigged system that gets confusing when you have 20 plates of 3 different strains. It barely works. I obviously list the strain and date of transfer/inoculation, but am uncertain of how to track transfers/cloned strains.
I'm sure the pros deal with palette-loads worth of petris and manage to keep track it all. How do they do it?
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strangladesh
masterOFpuppets



Registered: 10/17/05
Posts: 815
Loc: Right Here!
Last seen: 16 years, 1 month
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Re: "Proper" nomenclature for labeling agar plates/strains [Re: Hafrican]
#6834322 - 04/25/07 08:44 PM (16 years, 9 months ago) |
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i use 1a for multi, then 2a for the transfer, 1b would be my clones and 2b if i have another clone transfer...
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xaxphaanes
Mycologist


Registered: 08/08/05
Posts: 2,988
Last seen: 1 year, 6 months
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Re: "Proper" nomenclature for labeling agar plates/strains [Re: strangladesh] 1
#6834422 - 04/25/07 09:06 PM (16 years, 9 months ago) |
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i use that same probe usualy i have to of course get the casing mix ready and moist and then stick it in there for like 10 mins to get a good reading just takes some time to get a good reading with these probes i would get a digi probe those are good
-------------------- "Anything i say is fictional" what you should look for in manure
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strangladesh
masterOFpuppets



Registered: 10/17/05
Posts: 815
Loc: Right Here!
Last seen: 16 years, 1 month
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Re: "Proper" nomenclature for labeling agar plates/strains [Re: xaxphaanes] 1
#6834859 - 04/25/07 10:39 PM (16 years, 9 months ago) |
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Quote:
xaxphaanes said: i use that same probe usualy i have to of course get the casing mix ready and moist and then stick it in there for like 10 mins to get a good reading just takes some time to get a good reading with these probes i would get a digi probe those are good
WTF are you talking about...
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 22 days
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Re: "Proper" nomenclature for labeling agar plates/strains [Re: strangladesh] 2
#6835335 - 04/26/07 01:14 AM (16 years, 9 months ago) |
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Everybody ends up inventing their own system after awhile and that's fine.
My system is to list the date first, parent species second, then strain third, and then each transfer beginning with the letter 'A' and going through the alphabet. Assume the species is Reishi, and the strain is my 'sheriff'.(sherrif strain got its name because a dumbass sheriff gave me a bogus ticket the day I cloned it, but that's another story) A swipe of spores made today on agar would be labeled 042607RS.
Assuming three days from now I make a series of transfers of the first mycelium to germinate from spores, they would be labeled 042907RS A1, B1, C1, D1, etc. As the strains differentiate, the next series of transfers would be labeled (date)RS-A1-A, (date)RS-A1-B, etc. From the second original dish, the second set of transfers would be (date)RS-B1-A, (date)RS-B1-B, etc and right on down the list. It's important to be consistant with your system all the way to fruiting because when you find that awesome fruiting strain three months from now, you want to be able to go back to the original dish in the refrigerator that corresponds to the fruiting tray, pull it out and make a master culture slant from it. That way, you preserve the genetics of a great strain from a very early time in its life before many cell divisions have occured. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Hafrican
Mega-ultrachicken

Registered: 01/31/03
Posts: 105
Loc: SW Azerbaijan
Last seen: 16 years, 7 months
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Re: "Proper" nomenclature for labeling agar plates/strains [Re: RogerRabbit]
#6835756 - 04/26/07 06:54 AM (16 years, 9 months ago) |
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Quote:
RogerRabbit said: It's important to be consistent with your system all the way to fruiting because when you find that awesome fruiting strain three months from now, you want to be able to go back to the original dish in the refrigerator that corresponds to the fruiting tray, pull it out and make a master culture slant from it.
That has been my problem. (Or one of many to be more humble). Tracing the strain from fruiting back to the plates. I've done some quick-and-dirty multispores to get to fruiting quickly, then clones of some of those fruits. Surprisingly, some of the multispore inoculations end up being as productive as the isolates, but I attribute that to re-learning some of the subtleties that I've forgotten in the three years since I last delved into this process.
Thank you. I'll give back some hopefully with a grow log soon. I'm resuscitating some four year old prints that were moved two times. CR and MAZ. They've all germinated so far! Goes to show you that proper technique and storage will keep the spores viable for at least that period of time.
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 22 days
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Re: "Proper" nomenclature for labeling agar plates/strains [Re: Hafrican] 2
#6836276 - 04/26/07 10:14 AM (16 years, 9 months ago) |
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Most multispore projects will be more productive than most isolates. The differences is a FEW isolates will be killer, and those are the ones you want to save. You don't want to clone from the best fruiter, you want to go back to the original isolate in the refrigerator so you can get the youngest cell line. A properly prepared set of master culture slants will last a lifetime of growing without degradation. I show the process for strain isolation and making master culture slants on my DVD by the way.(shameless plug) RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Hafrican
Mega-ultrachicken

Registered: 01/31/03
Posts: 105
Loc: SW Azerbaijan
Last seen: 16 years, 7 months
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Re: "Proper" nomenclature for labeling agar plates/strains [Re: RogerRabbit]
#6837969 - 04/26/07 05:28 PM (16 years, 9 months ago) |
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Hahahaha.
I've been following that project and will be very excited when it is released! I can let the plug slide .
Friends do video/dvd production, so I'm aware of the absurd amounts of time involved. Inherent resolution differences based on country, tweaking encoding for maximum content: it's undoubtedly a bitch. From what I've heard, sound can be especially nightmarish.
I'll buy it alone based on the isolation/slant content. Not to knock the hobby, but, like 16 year olds can get a yield, inoculating jars under their parent's sink where they stash their "pr0n." You get to a certain point, and the question "what am I trying to get out of this?" most likely comes up.
But I wander. Thanks for the assistance. I'm going to try and revive two contaminated plates started from spore. Hafrican's got another few before he's working 60 hour weeks and getting 150 phone calls a day. What better way to spend that time then getting the summer edibles going and blowing through a few sleeves?
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