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Offlinecrazygirl96
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Registered: 04/17/07
Posts: 17
Last seen: 17 years, 6 months
tape question
    #6836941 - 04/26/07 01:34 PM (17 years, 8 months ago)

last night i had put tape over my holes and then steamed them, so when it was time to inject the spores i just put the needle through the tape, will this cause contams?

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Offlineeyesoftheworld
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Re: tape question [Re: crazygirl96]
    #6836983 - 04/26/07 01:44 PM (17 years, 8 months ago)

Nope, as long as you flame sterilized and/or put an alcohol soaked towel around the needle.


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:deemsters:

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Invisiblemushbaby
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Re: tape question [Re: eyesoftheworld]
    #6837300 - 04/26/07 03:07 PM (17 years, 8 months ago)

I don't know if it"s overkill or not but I also take a cotton ball soaked in alcohol and wipe the top of the jars.


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Offlinekyleda1kid
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Registered: 03/27/07
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Re: tape question [Re: eyesoftheworld]
    #6837309 - 04/26/07 03:08 PM (17 years, 8 months ago)

i did the same exact thing, and jars are looking good so far, distict white mycelium patches in all four areas of the holes. Hope you added top layer of dry verm. to your cake to help with contams. Assuming you followed the pf-tek.

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OfflineCheesekiller
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Loc: Central NY
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Re: tape question [Re: kyleda1kid]
    #6837338 - 04/26/07 03:14 PM (17 years, 8 months ago)

You could re-tape the holes, but if you think they look wet make sure to use some porous tape or a band-aid works good. The verm top should work well to block the contams but extra caution always helps.


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Bulk grower with "some" success.

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Nice Lids

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Offlinekyleda1kid
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Re: tape question [Re: kyleda1kid]
    #6837339 - 04/26/07 03:14 PM (17 years, 8 months ago)

wipe jars, b4 or after pressure cooking? in pf-tek they say just use your finger to wipe substrate off top of jars b4 cooking. Helps prevent contaminants from following sloppy substrate into the jars causing contams.

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Offlinekyleda1kid
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Re: tape question [Re: kyleda1kid]
    #6837354 - 04/26/07 03:16 PM (17 years, 8 months ago)

i wouldn't re tape- i asked a question about leaving foil over tops of jars and even taping the foil to the jars to help prevent contams. and learned that you need some air to the jars, so i wouldn't re-tape.

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Invisiblemushbaby
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Re: tape question [Re: kyleda1kid]
    #6837375 - 04/26/07 03:21 PM (17 years, 8 months ago)

wipe the lids just before innoculating. But I am a noob myself. Just a couple of grows. There's an article on sterility tech in mush cult that talks about this pretty thoroughly.


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Offlinekyleda1kid
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Re: tape question [Re: mushbaby]
    #6837492 - 04/26/07 03:49 PM (17 years, 8 months ago)

I wouldn't wipe lids off, that's why you PC, and sterilize needle b4 innoc. Wiping it with what you think is clean will probly just put bacteria on top of the jars. Again that is why you PC them.

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