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OfflineMrCubensis
shut em down!
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Registered: 02/12/07
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improper LC???(help with WBS+LC)
    #6835943 - 04/26/07 08:23 AM (17 years, 8 months ago)

So my WBS jars are taking forever to colonize, i inoculated them about 4-5 days ago with LC made from multispore syringe..

i have barely any growth in the jars, some jars have like 3 little puff spots of myc no bigger then the tip of a pen,

i tried searching posts but most answers have to do with contam..and i doubt its contams I'm thinking maybe my LC is not good?

its basically poor mans LC , bottle honey+water,microwaved for 5min then another 5...

i have good healthy looking MYC growing in the LC but idk if maybe its taking a long time for a certain reason or what...

the only other thing i can think of is my WBS is to dry...before i had problems getting moisture content right with my jars (to wet) so i used some drying methods this time on some of the jars(paper towel/blow dryer for a few min)

should i take one jar and squirt a few cc's more of LC or try in jecting a little bit of sterile h2o?

any help would greatly be appreciated
thanks in advanced!


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"The Golden Rule: He who has the gold, Creates the rule"

http://educate-yourself.org/ct/

Edited by MrCubensis (04/26/07 01:01 PM)

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OfflineMrCubensis
shut em down!
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Registered: 02/12/07
Posts: 287
Loc: some where in the ocean
Last seen: 11 years, 10 months
Re: improper LC???(help with WBS+LC) [Re: MrCubensis]
    #6836814 - 04/26/07 01:02 PM (17 years, 8 months ago)

bump, anyone?


--------------------


"The Golden Rule: He who has the gold, Creates the rule"

http://educate-yourself.org/ct/

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OfflineMrCubensis
shut em down!
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Registered: 02/12/07
Posts: 287
Loc: some where in the ocean
Last seen: 11 years, 10 months
Re: improper LC???(help with WBS+LC) [Re: MrCubensis]
    #6837601 - 04/26/07 04:16 PM (17 years, 8 months ago)

no body on these great forums can help a brotha out?


--------------------


"The Golden Rule: He who has the gold, Creates the rule"

http://educate-yourself.org/ct/

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Offlinekyleda1kid
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Registered: 03/27/07
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Re: improper LC???(help with WBS+LC) [Re: MrCubensis]
    #6837663 - 04/26/07 04:34 PM (17 years, 8 months ago)

dude, wait longer than 3-4 days! More like 3-4 weeks!!

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OfflineMrCubensis
shut em down!
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Registered: 02/12/07
Posts: 287
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Last seen: 11 years, 10 months
Re: improper LC???(help with WBS+LC) [Re: kyleda1kid]
    #6838004 - 04/26/07 05:39 PM (17 years, 8 months ago)

3-4 weeks for first signs of mycelium growth...i think you miss read

not 3-4 days until full colonization
haha

most LC's are done colonizing within a week to 10 days, its been half that and i only have specks of myc in like 3 places..??


--------------------


"The Golden Rule: He who has the gold, Creates the rule"

http://educate-yourself.org/ct/

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Offlinekyleda1kid
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Re: improper LC???(help with WBS+LC) [Re: MrCubensis]
    #6838024 - 04/26/07 05:45 PM (17 years, 8 months ago)

who are you talking to. it will be 3-4 weeks for full colonization.

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OfflineJazzCatCF
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Re: improper LC???(help with WBS+LC) [Re: MrCubensis]
    #6838038 - 04/26/07 05:48 PM (17 years, 8 months ago)

Yea it takes me 3 weeks with a spore syringe.It should be way faster witha lc.Im getting ready to do my 1st lc,and from what I read 3 to 5 days youll start seeing growth,then it will explode,and youll have mycelium everywhere in the jar.


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"There is a world beyond ours, a world that is far away, nearby, and invisible. And there it is where God lives, where the dead live, the spirits and the saints, a world where everything has already happened and everything is known. That world talks. It has a language of its own. I report what it says. The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me. When I return from the trip that I have taken with them, I tell what they have told me and what they have shown me."

The Mazatec Wise Woman, Maria Sabina (1894-1985)

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OfflineWronguy

Registered: 03/05/05
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Trusted Cultivator
Re: improper LC???(help with WBS+LC) [Re: kyleda1kid]
    #6838156 - 04/26/07 06:30 PM (17 years, 8 months ago)

I'm sorry kyleda1kid, but you're wrong. You will easily have full colonization within 7-10 days using a liquid culture inoculate. Obviously the time depends on the amount of liquid culture used, but that's a pretty fair average.

Mr.Cubensis, your problem may have to do with the moisture content. If your moisture content is too high or too low you are likely to see slow colonization times. I always run my WBS on the dry side when I'm using a liquid culture, as the addition of the culture will add what I need.

There is also some more information that could be used here such as technique for WBS preparation, amount of liquid culture used, and incubation temps. What are you using to allow CO2 expulsion. Poor gas exchange is also a leading cause of slow colonization.

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Offlinekyleda1kid
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Registered: 03/27/07
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Re: improper LC???(help with WBS+LC) [Re: Wronguy]
    #6838181 - 04/26/07 06:42 PM (17 years, 8 months ago)

yeah, i do appologize i was thinking of cakes, pf-tek style. listen to Wronguy.

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Offlinedizzy_jester
I aint noclown...


Registered: 03/16/07
Posts: 158
Last seen: 15 years, 10 months
Re: improper LC???(help with WBS+LC) [Re: kyleda1kid]
    #6838205 - 04/26/07 06:51 PM (17 years, 8 months ago)

what is your incubation temp at and what type of jars are you using ( tyvek, open holes with poly)

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OfflineMrCubensis
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Registered: 02/12/07
Posts: 287
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Last seen: 11 years, 10 months
Re: improper LC???(help with WBS+LC) [Re: dizzy_jester]
    #6838402 - 04/26/07 07:48 PM (17 years, 8 months ago)

dont worry kyledakid its cool, anyways im actually expiramenting with different things,

i have to jars at room temp wich ranges from low to high 70's depending on the weather (my room has horrible insilation)

and the other jars i have in my incubator at around 77-80, most of my jars are on the dry side, they lida are with 2-4 holes aind a coffee filter undernieth the lids

i was thinking that in a few days if things are still slow in the dryer jars im gonna inject a few cc's of sterile water or maybe a little bit more LC but im unsure any sudjestions?

if you guys want in a few days i can post pics of the LC and the jars if you need to

thanks in advance


--------------------


"The Golden Rule: He who has the gold, Creates the rule"

http://educate-yourself.org/ct/

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