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InvisibleArp
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Registered: 04/20/98
Posts: 2,191
Loc: in a van by the river
fractal contamination picture
    #6734418 - 04/01/07 10:07 AM (17 years, 1 day ago)



Got this fractal contamination from a syringe I bought.

Guess it is some kind of bacteria. Is it possible to identify?
Perhaps I need to get a microscope :blush:

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InvisibleArp
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Re: fractal contamination picture [Re: Arp]
    #6734564 - 04/01/07 11:16 AM (17 years, 1 day ago)

Pseudomonas aeruginosa? :tongue:

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OfflineRogerRabbitM
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Re: fractal contamination picture [Re: Arp]
    #6735661 - 04/01/07 05:59 PM (17 years, 1 day ago)

I long ago gave up on trying to identify bacteria. I have a bunch of microscope slides I purchased from Boreal Labs for just that purpose, but it's a real pain in the ass. It IS bacteria though, so the plate is useless.

If you'll inoculate several dishes, you might find one where some mushroom spores germinate, and you can then transfer them to a new dish. By repeating the process a few times, you can usually isolate healthy mycelium away from contaminanants that way. I've been able to get a lot of my strains from contaminated syringes.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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InvisibleArp
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Re: fractal contamination picture [Re: RogerRabbit]
    #6737095 - 04/02/07 03:04 AM (17 years, 17 hours ago)

Yeah I squirted some more spore-solution onto a couple of dishes. Going to isolate the fuzz :smile:

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InvisibleAndroctonus
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Re: fractal contamination picture [Re: Arp]
    #6737101 - 04/02/07 03:12 AM (17 years, 17 hours ago)

I wonder if we dare ask where the syringe came from ?  :rolleyes:


--------------------
The red grass, up to my knees
An aura comes towards me

Someone's changed the formula
Un-chaining another law

The structures, the colors
Uniform chaos is alternating

The nature of things

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InvisibleArp
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Re: fractal contamination picture [Re: Androctonus]
    #6737206 - 04/02/07 05:35 AM (17 years, 14 hours ago)

I'll keep that a secret :smile:

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InvisibleArp
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Re: fractal contamination picture [Re: Arp]
    #6762002 - 04/08/07 04:45 PM (16 years, 11 months ago)



Here is a new one with more spore solution. I managed to isolate some mycelium and later rhizo! :thumbup:

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OfflineRogerRabbitM
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Re: fractal contamination picture [Re: Arp]
    #6762923 - 04/08/07 09:14 PM (16 years, 11 months ago)

Most of that mycelium looks more like mold. Are you sure it's rhizo? If it came from that, it's doubtful. The large spot at 7 o'clock looks like the best choice, but I dunno man. Doesn't look good.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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InvisibleArp
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Re: fractal contamination picture [Re: RogerRabbit]
    #6764027 - 04/09/07 04:31 AM (16 years, 11 months ago)

Yeah it looks pretty bad and wispy (cobweb?) but on another plate just
like this I isolated a part and took the rhizo on a new one :smile:


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OfflineRogerRabbitM
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Re: fractal contamination picture [Re: Arp]
    #6764675 - 04/09/07 10:40 AM (16 years, 11 months ago)

That looks better. It usually takes a few transfers to get completely away from the mold. Don't stop at one.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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InvisibleCorporal Kielbasa

Registered: 05/29/04
Posts: 17,235
Re: fractal contamination picture [Re: RogerRabbit]
    #6828980 - 04/24/07 07:49 PM (16 years, 11 months ago)

yeah i would only section of the couple tips on the few long strands that get out further then the wispy radial growth.

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InvisibleArp
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Re: fractal contamination picture [Re: Corporal Kielbasa]
    #6832554 - 04/25/07 01:42 PM (16 years, 11 months ago)

Seems like some strains I get won't rush into a fullfledge rhizo on
my MEA dishes no matter how many transfers I do, starting from clone or
multispore. Sometimes they revert to non-rhizo stage as well.
Maybe I should try a different agar recipe?

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InvisibleCorporal Kielbasa

Registered: 05/29/04
Posts: 17,235
Re: fractal contamination picture [Re: Arp]
    #6833278 - 04/25/07 04:26 PM (16 years, 11 months ago)

Try a few!

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Offlinenolongerinuse
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Re: fractal contamination picture [Re: Corporal Kielbasa]
    #6834501 - 04/25/07 09:21 PM (16 years, 11 months ago)

looks like you got Antarctica there buddy, You're gonna want to release mass amounts of greenhouse gases to rid yourself of that little problem.

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