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Offlinefromthemoon
ESP Major


Registered: 08/02/06
Posts: 353
Last seen: 17 years, 6 months
Making Prints
    #6827306 - 04/24/07 12:03 PM (17 years, 8 months ago)

Hey all,

I'm working on my spore print technique and have a few questions.

Sterility is always a passion for me, mainly to make sure my hard work doesn't go to waist. In following the teks, spore printing does not feel very serial to me.

I understand wiping down materials w/ alcohol, wearing gloves, mask, hair net, running a hepa, spraying room with oust.

The hard part is getting the actual cap.

It seems like allowing a shroom to mature up to the point of dropping spores, it is already exposed to air in the terrarium.

How much do I need to be worried about that?

Picking, cutting the stem, adding the push-pin, placing on the foil, and covering also seems like a lot of exposure to open air.

I started with spore prints and never ever was it exposed to open air. In fact, the only steps in this process where anything gets exposed to air is during spawning, casing, and fruiting.

It's hard for me to wrap my mind around making a spore print (the first step of the process) in open air.

I do have some small casings made specifically for printing that I plan to place in a monotub-like terrarium (sealed until ready to pick/print.

Even still, taking the cap and placing it on foil seems like a lot of time exposed to open air. (even if it is only one minute)

Any advice or input?

Thanks


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Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!

Edited by fromthemoon (04/24/07 12:06 PM)

Extras: Filter Print Post Top
InvisibleRoachMan
Old Man
Male User Gallery


Folding@home Statistics
Registered: 03/06/05
Posts: 2,083
Loc: Midwest
Re: Making Prints [Re: fromthemoon]
    #6827344 - 04/24/07 12:12 PM (17 years, 8 months ago)



--------------------
"The mistake that I make is to try to come awake in a place you're just supposed to get shit faced or baked."

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Offlinejohnnyboy
Bastard Son

Registered: 04/02/07
Posts: 644
Last seen: 12 years, 8 months
Re: Making Prints [Re: fromthemoon]
    #6827348 - 04/24/07 12:12 PM (17 years, 8 months ago)

you could do it in a glove box.


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I keep the bible in a pool of blood so that none of it's lies will affect me

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Offlinefromthemoon
ESP Major


Registered: 08/02/06
Posts: 353
Last seen: 17 years, 6 months
Re: Making Prints [Re: johnnyboy]
    #6827403 - 04/24/07 12:26 PM (17 years, 8 months ago)

I like Agar's tek, I'm afraid my glove box is too small to do all of that, but at least it deals with the sterility.

I'm going to try that today since I have a small tray 1 day from breaking the veils.


--------------------
Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!

Extras: Filter Print Post Top
InvisibleBridgeburner
Not spiritual at all.
Male


Folding@home Statistics
Registered: 09/16/06
Posts: 20,010
Re: Making Prints [Re: fromthemoon]
    #6827441 - 04/24/07 12:36 PM (17 years, 8 months ago)

rip out the mushroom, lay it on a wiped surface, cut through with a scalpel, pick cap up with pincers and place on a pre-sterilized foil w/ pre-sterilized glass that goes over it. and done.


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Invisibleagar
old hand
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Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
OG Cultivator
Re: Making Prints [Re: fromthemoon] * 1
    #6827484 - 04/24/07 12:47 PM (17 years, 8 months ago)

There are a few tricks about spore printing that come with experience.

I tend to print from second flushes.
Simply because fruit/caps are larger.
Which makes them easier to work with.
And, second flush caps seen to print FAR DARKER.


As fruits in a tray mature.
Watch them VERY CAREFULLY.

Just BEFORE veils break, drape a thin clear plastic bag over the tray.
(I use the thin see/through small waste paper trash can liners)

Doing so prevents FAE blowing contaminates into gills.
(as the veils tear open)
Remove the tray from the fruiting chamber.

Sanitize the exterior parts of the tray.
Place the covered tray in a sanitized still air glove box.
(assuming you have a large glove box).

Usually, in 24 hours, veils will tear & caps flatten out.

Have sanitized tools & a/foil squares prepared.
Stored in a large new baggy.

Have sanitized containers ready.


Inside glove box.
Lay a/foil squares in sanitized printing container.
Clip caps & place on a/foil squares.
Add lid, with gas exchange filter provision.
I use clear Sterilite containers, with lids.
I drill holes in the lids & cover holes with micropore tape.

Allow prints to SET 24 hours.

I, then remove print laying trays from glove box.
And, place containers in laminar flow-hood.


Then, cut, sanitize & clear laminate material into small squares.
Slightly larger than a/foil squares prints are layed on.


Then, remove cap from a/foil square.
Allow prints to dry 24 hours, after caps are removed.

Then, in F/H cover print with sanitized laminate material.
Then, quickly impulse seal each print.


After impulse sealing prints, I trim off excess laminate.

Finished.


--------------------

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InvisibleRoachMan
Old Man
Male User Gallery


Folding@home Statistics
Registered: 03/06/05
Posts: 2,083
Loc: Midwest
Re: Making Prints [Re: agar]
    #6827520 - 04/24/07 12:54 PM (17 years, 8 months ago)

You must have missed it; I already had a link to ya', buddy. :wink:

J/K, thanks for elaborating. :laugh:


--------------------
"The mistake that I make is to try to come awake in a place you're just supposed to get shit faced or baked."

Extras: Filter Print Post Top
Invisibleusg543
◕‿◕


Registered: 02/11/07
Posts: 5,192
Re: Making Prints [Re: agar]
    #6827530 - 04/24/07 01:00 PM (17 years, 8 months ago)

Quote:

agar said:






beautiful, i want!!

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Offlinefromthemoon
ESP Major


Registered: 08/02/06
Posts: 353
Last seen: 17 years, 6 months
Re: Making Prints [Re: usg543]
    #6831797 - 04/25/07 11:23 AM (17 years, 8 months ago)

Thanks Agar,

The new post is slightly updated from the link.

Thankfully I have a few trays to play with. I do have enough room in my glove box. But... I made some errors:

1) I sprayed down my glove box with alcohol and left wet (which I normally do)

2) I had some Damp Rid in my box that was not covered

It took only 3 hours before my shrooms turned dark blue - he he

Quote:

There are a few tricks about spore printing that come with experience.




That experience includes failures, like turning my shroomies blue; but now I know what not to do.

This tek should be added to the main site IMHO. It's the most serial tek for spore printing I've seen.

Thanks


--------------------
Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!

Edited by fromthemoon (04/25/07 11:24 AM)

Extras: Filter Print Post Top
Invisibleagar
old hand
 User Gallery


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
OG Cultivator
Re: Making Prints [Re: fromthemoon]
    #6831879 - 04/25/07 11:42 AM (17 years, 8 months ago)

Most folks don't have a flowhood to work in.
Not to mention an impulse sealer & laminating material.

A flowhood and impulse sealer is not required.

Simply use a glovebox.
And, fold squares of aluminum foil, to seal the prints.

The bag over unopened caps helps.
Having some sort of G/E filter, on the print container helps.
(caps perspire moisture)
Once caps are removed, don't forget to allow ample drying time.
Otherwise, wet prints can breed bacteria.

It all takes some practice.
But, once you get the hang of it.
It's fairly easy.


--------------------

Extras: Filter Print Post Top
Offlinefromthemoon
ESP Major


Registered: 08/02/06
Posts: 353
Last seen: 17 years, 6 months
Re: Making Prints [Re: agar]
    #6832202 - 04/25/07 12:41 PM (17 years, 8 months ago)

I figured the flow hood and laminator were optional - but it help to see a tek that is as sterile as one can get.

So far in my experiments:

The darkest prints I've created (although I don't trust those to be sterile) were created with the help of damp rid.

I placed an inch of damp rid in the bottom of a 1/2 pint jar. Then I placed a wire mess 1/2 inch over the damp rid. On that I placed my sterile foil, cap on top, and screwed on the lid.

It was air tight, but because of the damp rid it didn't need air exchange. - I guess


--------------------
Look at the atoms in the air and allow your mind to see the other planes that coexists in the same physical space where you sit. There are vibrational strings in each and every atom containing infinite realities, universes, and dimensions. Multidimensional beings and entities are RIGHT IN FRONT OF YOU!!! Only gravity and mental energy can pass through these different atomic branes and mushrooms can break the mental/spiritual membranes separating one reality from the other. TAKE A LOOK!

Extras: Filter Print Post Top
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