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OfflinesyntheticTHC
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Registered: 12/29/02
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Cooler, B+, First Time, Bags, Help?
    #6787393 - 04/14/07 03:10 PM (17 years, 9 months ago)

Hello friends,

This is my first time. I have a B+ syringe and 4 grain mycobags. I inoculated 2, put them in a blue rubbermaid with an aquarium heater in a large water-filled, with a dash of H2O2, glass jar-thing. The heater wasn't enough in the rubbermaid so I transferred them to a medium plastic cooler which then kept it at around 83 degrees. After awhile they started to grow, then they started to green, then they were completely green and trashed.

For my next 2 bags, I want to make sure I didn't do something wrong. I anti-bacteria my hands, burned and alcoholed the syringe (the syringe has a more blunt sized tip than I thought it would and when I first pushed it through the self healer it got plugged up, so i took it out and had to push the crud inside of it out with a tiny pin. This happened to both bags. I assume this may be the problem.), i covered the self-healer with tape after a quick alcohol rubdown, and then hoped it would work.

I am guessing that it was either the needles blunt tips fault or the fact that they started off a week in a 75 degree tank then moved to an 85 degree tank.

Should I find a pinpoint needle head to replace this slightly larger one, or will my 85 degree cooler setup do the trick? Am I missing something here?

Thanks.


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InvisiblePremedman1
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Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity
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Re: Cooler, B+, First Time, Bags, Help? [Re: syntheticTHC]
    #6787563 - 04/14/07 04:04 PM (17 years, 9 months ago)

Your syringe could be contaminated. What's its source?

75 degrees is fine for colonization. It couldn't have negatively affected your grow.

>>>Should I find a pinpoint needle to replace this slightly larger, blunt one?

Yes.


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OfflinesyntheticTHC
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Registered: 12/29/02
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Last seen: 9 years, 8 months
Re: Cooler, B+, First Time, Bags, Help? [Re: Premedman1]
    #6787596 - 04/14/07 04:11 PM (17 years, 9 months ago)

The syringe is over a year old and I received it in the marketplace. Would it be in my best interest to invest in an entirely new syringe?


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Offlinemockeylock
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Registered: 02/07/04
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Re: Cooler, B+, First Time, Bags, Help? [Re: syntheticTHC]
    #6787606 - 04/14/07 04:13 PM (17 years, 9 months ago)

Yes.


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OfflinesyntheticTHC
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Registered: 12/29/02
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Re: Cooler, B+, First Time, Bags, Help? [Re: mockeylock]
    #6787641 - 04/14/07 04:22 PM (17 years, 9 months ago)

Thanks for your simplistic and easy to understand answers.

Much appreciated!:heart:


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Offlinemockeylock
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Re: Cooler, B+, First Time, Bags, Help? [Re: syntheticTHC]
    #6787854 - 04/14/07 05:21 PM (17 years, 9 months ago)

No, but seriously...you didn't make it yourself and it didn't come from a vendor....

Not that that's a bad thing, necessarily, but if you feel you were sterile in your techniques and both contamed, then I'd try another source.

You'll never know for sure unless you keep playing with them, but why waste time, money, and effort if there's a decent chance the sringe is less than clean?

So, in short...
yes.


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OfflinesyntheticTHC
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Registered: 12/29/02
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Last seen: 9 years, 8 months
Re: Cooler, B+, First Time, Bags, Help? [Re: mockeylock]
    #6824453 - 04/23/07 06:49 PM (17 years, 8 months ago)

I wasn't trying to be sarcastic, I was actually thankful for the short answer. I took the advice and trying again with a new one today. Thanks again for the help.


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