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Offlineflipside13
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can you innoc agar from a pin or myc chunk?
    #6772496 - 04/10/07 09:12 PM (16 years, 11 months ago)

I just birthed a couple of cakes and got a myc chunk and an inch long pin that broke off. Can I plop them on agar or something and grow myc?
Flipside13


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Flipside13
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OfflineCarharttKidd
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Re: can you innoc agar from a pin or myc chunk? [Re: flipside13]
    #6772520 - 04/10/07 09:15 PM (16 years, 11 months ago)

If it broke off in front of a flow hood and fell into an pitri dish maybe.

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OfflineBlehMaestro
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Re: can you innoc agar from a pin or myc chunk? [Re: CarharttKidd]
    #6772583 - 04/10/07 09:25 PM (16 years, 11 months ago)

It would have to take place under those sterile conditions via glove box or flow hood. However, cloning from a pin from the first pinset is the way to go from what I learned in one of RR's threads today.

I was searching the forum for some topics and somehow I stumbled onto an experiment he ran where he put some pins into agar and showed us how they break down into mycelium very aggressively.

He said to use pins instead of mature fruit. I think you can also put a pin straight into grain and it will colonize it.

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OfflineCarharttKidd
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Re: can you innoc agar from a pin or myc chunk? [Re: BlehMaestro]
    #6772673 - 04/10/07 09:42 PM (16 years, 11 months ago)

An invitro pin from a PF cake would be perfect for this.

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OfflineRogerRabbitM
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Re: can you innoc agar from a pin or myc chunk? [Re: CarharttKidd]
    #6772748 - 04/10/07 09:55 PM (16 years, 11 months ago)

These pins below were from a normal indoor flush. They need not be sterile. Rapidly growing mycelium such as developing pins will outrun all contaminants. To perpetuate this strain, a tiny piece of mycelium from the leading edge of these growths was then transferred to new petri dishes to grow out.
RR

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OfflineBlehMaestro
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Re: can you innoc agar from a pin or myc chunk? [Re: RogerRabbit]
    #6772780 - 04/10/07 10:00 PM (16 years, 11 months ago)

Quote:

RogerRabbit said:
These pins below were from a normal indoor flush. They need not be sterile. Rapidly growing mycelium such as developing pins will outrun all contaminants. To perpetuate this strain, a tiny piece of mycelium from the leading edge of these growths was then transferred to new petri dishes to grow out.
RR

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That's sick RR! I thought I had read it was sterile procedure, but my bad!

I can't wait to try this...weren't you saying something about taking pins before something happened? I believe it was the transition where the mycelium decides which pins to mature and which not? I may be delusional, but I recall reading something about that.

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Offlineflipside13
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Re: can you innoc agar from a pin or myc chunk? [Re: BlehMaestro]
    #6773108 - 04/10/07 11:07 PM (16 years, 11 months ago)

So, I have one pin that broke off after dunking and rolling and each cake has 3-5 one inch pins that had grown invitro. They are hanging by strings of myc and don't look very viable. I am thinking I should pick them off and use them to grow in agar. What say you RR? You still out there? Also, I will be making up p-dishes in the next few days - can I just put the pins in the fridge to keep em dormant?
Flipside13


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OfflineRogerRabbitM
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Re: can you innoc agar from a pin or myc chunk? [Re: flipside13]
    #6773478 - 04/11/07 12:28 AM (16 years, 11 months ago)

No, they need to right from substrate to agar while stil in a rapid state of growth. If you put them in the refrigerator, they'll slow down their rate of growth, then when you put them on agar, the molds will recover faster. Next time, have some petri dishes already poured and ready to go.
RR


--------------------
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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Offlineflipside13
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Re: can you innoc agar from a pin or myc chunk? [Re: RogerRabbit]
    #6774267 - 04/11/07 09:18 AM (16 years, 11 months ago)

Gotcha. I will take a few pins direct from the cakes when I get my plates made up. Thanks again for the guidance.
Flipside13


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Offlineflipside13
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Re: can you innoc agar from a pin or myc chunk? [Re: flipside13]
    #6820971 - 04/22/07 09:30 PM (16 years, 10 months ago)

Hey RR,

I have a bunch of pins starting in a couple of jars that I am about to birth and I have not had great luck in those pins turning into viable umbrella tops, so I am thinking of using them for agar plates. Are invitro pins likely to work as well as pins from birthed cakes?
Flipside13


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OfflineRogerRabbitM
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Re: can you innoc agar from a pin or myc chunk? [Re: flipside13]
    #6820987 - 04/22/07 09:37 PM (16 years, 10 months ago)

Yes, but it sounds like bad genetics if they don't develop. Perhaps they're not the best choice for agar.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Offlineflipside13
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Re: can you innoc agar from a pin or myc chunk? [Re: RogerRabbit]
    #6821296 - 04/22/07 11:10 PM (16 years, 10 months ago)

hmm...good point. Would part of the problem with dying pins be dunking? My plan this time was to pull the pins of the cake for agar plates then dunk the cake. Whadda ya think?
Flipside13
BTW-Thanks for the quick reply!


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OfflineRogerRabbitM
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Re: can you innoc agar from a pin or myc chunk? [Re: flipside13]
    #6821375 - 04/22/07 11:40 PM (16 years, 10 months ago)

I've dunked cakes and even bulk substrates many times with pins on them. In fact, I've had a pan stipticus sawdust/woodchip block with hundreds of pins dunking all evening this very day. They usually double in size overnight during the dunk. I've never seen a pin abort from dunking, provided you allow plenty of air exchange after the dunk, so they can dry off over the first hour or two.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Offlineflipside13
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Re: can you innoc agar from a pin or myc chunk? [Re: RogerRabbit]
    #6822210 - 04/23/07 08:20 AM (16 years, 10 months ago)

Gotcha. Thanks again.
Flipside13


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