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OfflineSuchSmartMonkeys
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how much from each syringe in each jar?
    #6813322 - 04/20/07 03:09 PM (17 years, 8 months ago)

I'm about to inoculate my quart jars, and this is my first time growing...i have live culture syringes, not spore syringes, and i hear you need less. I used RR's rye tek, and have my jars all PCed up, and ready to go, i just need to know how much i should put in from each syringe into each jar?


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Offlinedizzy_jester
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Re: how much from each syringe in each jar? [Re: SuchSmartMonkeys]
    #6813365 - 04/20/07 03:28 PM (17 years, 8 months ago)

that is up to you, the more you put in the faster it will colonize. most poeple put in around 1-3 cc's of LC, just make sure you dont create a pool of water at the bottom of the jar and to shake the shit out of that jar to spread out the LC to as much rye as possible

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InvisibleRoachMan
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Re: how much from each syringe in each jar? [Re: dizzy_jester]
    #6813404 - 04/20/07 03:44 PM (17 years, 8 months ago)

Like he said...try to avoid a pooling.

If you are inoculating grains then a tiny pool is more forgiving as you can shake the jar to coat every kernal.
This, IME, leads to the fastest colonization times from LC.

If using PF tek style jars then only inoculate enough so that the fluid runs down almost to the bottom of the jar,
but falls about 1/4-1/2" short of the bottom (in each hole).


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OfflineSuchSmartMonkeys
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Re: how much from each syringe in each jar? [Re: RoachMan]
    #6813448 - 04/20/07 04:15 PM (17 years, 8 months ago)

hmm, maybe i hould have put in a little more. i ended up putting in about 1.5 CCs in each, maybe a little less, but well over 1 for each... I made sure to shake the shit out of the jars a good bunch so the grains looked all mixed up and there was no pooling anywhere. There was slight condensation on the sides after shaking...i just got tired of waiting, and went ahead and did it. hopefully it works out. well! i'm offto smoke some more herb!! happy 420 everyone!!


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OfflineCantiSama


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Re: how much from each syringe in each jar? [Re: SuchSmartMonkeys]
    #6813462 - 04/20/07 04:29 PM (17 years, 8 months ago)

i've inoculated with as much as 10cc of live culture in a quart of rye. although in retrospect this probably did hinder growth a tad by altering the moisture level the jars colonized just fine.


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OfflineSuchSmartMonkeys
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Re: how much from each syringe in each jar? [Re: CantiSama]
    #6824621 - 04/23/07 07:42 PM (17 years, 8 months ago)

at the amount of spores i put in there, how long should it be before i see signs of colonization in my jars? i did it on friday, 4/20...


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InvisibleOptx
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Re: how much from each syringe in each jar? [Re: SuchSmartMonkeys]
    #6825062 - 04/23/07 09:28 PM (17 years, 8 months ago)

should see growth some time today-wednesday


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OfflineSuchSmartMonkeys
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Re: how much from each syringe in each jar? [Re: Optx]
    #6827722 - 04/24/07 02:00 PM (17 years, 8 months ago)

it's only tuesday 4/24 here man....i don't know where you're at. I don't know if i have them in a warm enough area, it's probably around 67-70 degrees about...should i just move them to my bedroom and but them on a high up shelf and leave the heat on pretty low for a few days, see if that helps?


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OfflinePeteomighty
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Re: how much from each syringe in each jar? [Re: SuchSmartMonkeys]
    #6827971 - 04/24/07 03:05 PM (17 years, 8 months ago)

i think i should have shaken mine up after innoculating, because right now it's only colonised at the bottom and it's really white, but it's taking a long time to spread. will it eventually colonise the whole jar though?


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OfflineSuchSmartMonkeys
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Re: how much from each syringe in each jar? [Re: Peteomighty]
    #6828145 - 04/24/07 04:06 PM (17 years, 8 months ago)

i spoke a little too soon! i just went and took a look at my jars, and there is without a doubt nice white mycelium strands starting to form!! Nohing looking like contams, and it seems to be pretty evenly mixed, there is multiple spots throughout each jar that it seems to be colonizing from...I got through looking at all of the jars, and didn't notice anything until the last one (one of my P. ostreatus jars) had a nice big spot that seemed to be casting out strands, so i went back and looked at the others and found a bunch more mycelium that i hadn't seen before. i'll have a pic up in a little bit here...


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OfflineSuchSmartMonkeys
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Re: how much from each syringe in each jar? [Re: SuchSmartMonkeys]
    #6828177 - 04/24/07 04:14 PM (17 years, 8 months ago)

Here it is! my first signs of life!


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InvisibleBlutjager
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Re: how much from each syringe in each jar? [Re: CantiSama]
    #6828206 - 04/24/07 04:22 PM (17 years, 8 months ago)

Quote:

CantiSama said:
i've inoculated with as much as 10cc of live culture in a quart of rye. although in retrospect this probably did hinder growth a tad by altering the moisture level the jars colonized just fine.




I have done this too,more just for the hell of it than for any other reason,I find 4 cc's will do a nice job on qts of rye:thumbup:

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OfflineSuchSmartMonkeys
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Re: how much from each syringe in each jar? [Re: Blutjager]
    #6828553 - 04/24/07 05:54 PM (17 years, 8 months ago)

i only had 10 CCs of each, and was hoping to possibly get one more grow out of it...it seems to be working fine though, got my first signs of life today...i'm kind of half thinking about trying to PC the last 4 jars i have with some more rye and then just use the amount i used for the first 8 on just 4 (so twice as much) and try to get them to catch up...might just wait to see if this is a successful mission as well.


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