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Offlinesomebody041
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Re: HYDRATING & PASTEURIZING BULK SUBSTRATE PROPERLY [Re: KaptKid]
    #7836441 - 01/05/08 04:54 PM (16 years, 2 months ago)

captain cubensis why did you bump a thread without adding anything relevant at all?

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OfflineNibin
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Re: HYDRATING & PASTEURIZING BULK SUBSTRATE PROPERLY [Re: Captain Cubensis]
    #7838200 - 01/06/08 04:18 AM (16 years, 2 months ago)

Fourth (or maybe fifth) ancient thread you have bumped by quoting someone else in the thread without adding any new info.


What are you doing captain cubensis? Trying to increase your post count? Go to the pub or OTD for that.


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Re: HYDRATING & PASTEURIZING BULK SUBSTRATE PROPERLY [Re: Nibin]
    #8036638 - 02/18/08 04:47 AM (16 years, 1 month ago)

I just want to know if you can do this with coir and amendments and if so, what do you need to do with the coir first off? Should you let it expand with hot water and then bag load and pasteurize? Any help would be appreciated as I'm getting ready to do a spawn run in a bit. Thanks all!

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Re: HYDRATING & PASTEURIZING BULK SUBSTRATE PROPERLY [Re: BigBlack81]
    #16114081 - 04/19/12 10:37 PM (11 years, 10 months ago)

Yes this thread is old. Why your not supposed to post in still relevant amazing old posts is a mystery to me. Not sure on the etiquette but dammit man this is just to good to not have in my thread list for the rest of my existence on planet earth. I need it, I want it, and fuck yeah I have it! FYI the best post Ive seen on the subject yet!

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Re: HYDRATING & PASTEURIZING BULK SUBSTRATE PROPERLY [Re: KaptKid]
    #16950788 - 10/02/12 09:07 AM (11 years, 5 months ago)

I'm beginner, please don't judge my questions:
1. what is substrate?
2. where I can find step by step instructions how to get mushrooms from bulk grain? (I'm planning to use brown rice grain).

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Re: HYDRATING & PASTEURIZING BULK SUBSTRATE PROPERLY [Re: Legend9123]
    #18295017 - 05/20/13 09:24 PM (10 years, 9 months ago)

Great post very informative.  I'm hoping someone can clear something up for me.  In an effort to bulk grow using the techniques above the following steps should be followed. 

1.  Colonize Rye berries
2.  Pasteurized bulk substrate.  (I would like to use the method above with the following recipe 65% Coir / 20% Vermiculite(for better water retention, it also fluffs up the substrate a bit more and lets the mycelium rip through it faster) / 10% Coffee (just for a diversified substrate) / 5% Gypsum (this improves fruit body formation and has needed nutrients
3. (and this is when I get fuzzy) I need  to sterilize a room before opening the bag (or lid when using pot to pot)so that I can add the Rye berries to the substrate correct? Or is that not an issue as the Rye seed is already colonized. 
4.  After they Rye seed and the bulk substrate are mixed I must I must incubate with a casing layer correct? (or is this not added until fruiting stage?) I'm thinking of using vermiculite for this but I'm wondering if it needs to be sterilized\pasteurized\pressure cooked as well? 
5.  I need to improve upon the fruiting conditions I provided my last time out the gate.  My biggest issue is how to I keep a contained unit (for stealth) the has a 12 hour light cycle but ensure the temp doesn't exceed 72-74 degrees


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Re: HYDRATING & PASTEURIZING BULK SUBSTRATE PROPERLY [Re: mrmcchickentrouser]
    #18295063 - 05/20/13 09:34 PM (10 years, 9 months ago)

1 and 2 sound good.  Just be sure to pasteurize properly.  3.) When spawning to a bulk substrate it's good to alcohol bomb the room, take a shower, wear clean clothes, and brush your teeth right before beginning to build substrates.  4.)  NO need for incubation.  Room temperatures are best for colonization, and cubensis fruit just fine with out a casing layer.  4.)  Use a air conditioner.  The light cycle doesn't have to be exactly a 12 on and 12 off photo-period.  There just needs to be some dark and light time on a routine schedule to establish a good circadian rhythm.

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Re: HYDRATING & PASTEURIZING BULK SUBSTRATE PROPERLY [Re: hamloaf]
    #18295104 - 05/20/13 09:42 PM (10 years, 9 months ago)

Quote:

hamloaf said:
1 and 2 sound good.  Just be sure to pasteurize properly.  3.) When spawning to a bulk substrate it's good to alcohol bomb the room, take a shower, wear clean clothes, and brush your teeth right before beginning to build substrates.  4.)  NO need for incubation.  Room temperatures are best for colonization, and cubensis fruit just fine with out a casing layer.  4.)  Use a air conditioner.  The light cycle doesn't have to be exactly a 12 on and 12 off photo-period.  There just needs to be some dark and light time on a routine schedule to establish a good circadian rhythm.




3) I'd say sterile procedure doesn't matter much for spawning, fully colonized spawn is contam resistant n properly pasteurized sub is contam resistant.

Also. Mrmcchickentrouser try not to resurrect old thread u can just make a new one and link it if u have questions about an old thread


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Re: HYDRATING & PASTEURIZING BULK SUBSTRATE PROPERLY [Re: smegzilla69]
    #18295123 - 05/20/13 09:45 PM (10 years, 9 months ago)

4)welcome to shroomery :headbang3:


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Re: HYDRATING & PASTEURIZING BULK SUBSTRATE PROPERLY [Re: smegzilla69]
    #18295259 - 05/20/13 10:07 PM (10 years, 9 months ago)

Quote:

smegzilla69 said:

3) I'd say sterile procedure doesn't matter much for spawning, fully colonized spawn is contam resistant n properly pasteurized sub is contam resistant.

Also. Mrmcchickentrouser try not to resurrect old thread u can just make a new one and link it if u have questions about an old thread



5.)  Sterile procedure matters enough when spawning to bulk that he alcohol bomb the room, take a shower, wear clean clothes, and brush his teeth right before beginning to build substrates though and is why I prescribed it.

6.)  This is great thread and I for one am glad it was bumped and that I could be a part of it.  Also, it shows that some of the new guys are still using the search function.

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Re: HYDRATING & PASTEURIZING BULK SUBSTRATE PROPERLY [Re: hamloaf]
    #18295276 - 05/20/13 10:10 PM (10 years, 9 months ago)

Quote:

hamloaf said:
Quote:

smegzilla69 said:

3) I'd say sterile procedure doesn't matter much for spawning, fully colonized spawn is contam resistant n properly pasteurized sub is contam resistant.

Also. Mrmcchickentrouser try not to resurrect old thread u can just make a new one and link it if u have questions about an old thread



5.)  Sterile procedure matters enough when spawning to bulk that he alcohol bomb the room, take a shower, wear clean clothes, and brush his teeth right before beginning to build substrates though and is why I prescribed it.

6.)  This is great thread and I for one am glad it was bumped and that I could be a part of it.  Also, it shows that some of the new guys are still using the search function.




sorry u just seem to disagree with most of the TCs on the site... I'm simply parroting.

I don't alchi bomb or take a shower or brush my teeth b4 spawning
I wear glove because I don't really care for horse shit all love my hands n that's about it... My grows do fine...


--------------------

EVERYTHING I SAY IS A LIE!!! N ALL MY PICS R STOLEN
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SO DOPE
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Re: HYDRATING & PASTEURIZING BULK SUBSTRATE PROPERLY [Re: smegzilla69]
    #18295325 - 05/20/13 10:19 PM (10 years, 9 months ago)

Quote:

smegzilla69 said:
sorry u just seem to disagree with most of the TCs on the site... I'm simply parroting.




Oh?  When was the last time you've got me disagreeing with a "TC"?  Do you have a link or an example?  Besides, not advising someone to take any precautionary measure that they can because your grows supposedly turn out fine without them is in my opinion bad advice giving and slightly apathetic.

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Re: HYDRATING & PASTEURIZING BULK SUBSTRATE PROPERLY [Re: smegzilla69]
    #18295330 - 05/20/13 10:20 PM (10 years, 9 months ago)

agar was a great man and it's nice to see one of his posts up:thumbup:


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Re: HYDRATING & PASTEURIZING BULK SUBSTRATE PROPERLY [Re: cronicr]
    #18295365 - 05/20/13 10:27 PM (10 years, 9 months ago)

Very nice!!!!!!

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Re: HYDRATING & PASTEURIZING BULK SUBSTRATE PROPERLY [Re: caveman5055]
    #18295385 - 05/20/13 10:31 PM (10 years, 9 months ago)

Quote:

FrankHorrigan said:
Yes, you can. Similar yields though, that's real hard to say. I would hedge my bets on better yield from two different smaller tubs though.

And the shroomery is being weird for some pages today so here's my guide for ya:

Quote:

FrankHorrigan said:
This is part of my series on how I get things done. I hope it helps!

Quote:

RogerRabbit said:
Once your spawn jars are fully colonized, you can scratch your butt while you inoculate the coir if you want.




I will be using 11 quart jars of substrate I pasteurized using my pasteurization tek.
I will be using 7 quart jars of fully colonized WBS I prepped using my WBS tek.
This fills my 18gal monotubs up to the 3" mark, which I find is perfect. You can use a deeper substrate but I find 3" substrates perform as well as 4".

Let's clear one thing up right away:

Quote:

RogerRabbit said:
When it comes to spawning a bulk substrate, who gives a shit if you pick your nose while working?  It doesn't matter.  If the grains were properly prepared and colonized in sterile conditions to full colonization, you can spawn to bulk outdoors if you want to.  When you go to the Stamets seminars, you'll see Paul build a straw log or other project outside in the open air and it works fine.  However, you can bet your ass the spawn wasn't produced outdoors in the open air.




If your grain spawn is clean and fully colonized and you have properly pasteurized your substrate, you will not see a contam before the first flush.

Therefore, I spawn in open air with minimal precautions. I put on a pair of disposable gloves (non-sterile) and that is that. You can spawn clothed or you can spawn naked, it doesn't matter. No lysol, no oust, no extra cleaning beyond the usual that gets done once or twice a week in my house. I turn off the fans in the room but even that is unnecessary :thumbup:

I should mention that if you use the bucket tek, it is possible that you will see a contam before the first flush even though your spawn was clean. I never trust the bucket tek, since I live in a house with many pets and have had contam problems in the past.


First, wash the monotub out with warm soapy water. I wipe down the sides and bottom and lid. I also wipe down the outside. Dry it off with clean paper towels.

I line the inside of my tubs to prevent side pinning. Some will argue whether side pinning is acceptable or not, but I've found it's a real PITA for harvesting without ripping chunks from the substrate. Even when cutting at the base (which I recommend), side pins make everything messy.

I use thick plastic sheeting found in the paint section of any hardware store. I like a thick liner (3.5mil usually) to prevent tears.

Cut your liner to shape and place it in the tub. Make sure to use extra so you can mix your substrate up without spilling any. You'll be able to trim it after spawning.


I put my duct tape on the bottom holes of the mono and use it to hold the liner in place while working.


Take your colonized spawn jars, check for visible problems and break each one up. Pound it on something with a little give- I use the carpet, RR uses a bike tire.


Open each one and give it a good smell to make sure it is clean.
MAKE ABSOLUTELY SURE YOU SMELL EACH AND EVERY JAR OF SPAWN BEFORE DOING ANYTHING ELSE. This is the most important part.
You will not always be able to ID contaminants visually by inspecting the spawn jars. You will always be able to smell a problem though.
If it smells like ANYTHING except fresh mushrooms, DO NOT USE THE JAR. Toss it. Common smells that indicate contamination include the smell of dirt, a sour smell, a sweet smell, and/or a disgusting smell.

After smelling your jars, we will re-hydrate the grains before spawning. This improves the yield and quality of the fruits, and provides a quicker colonizing time.

Fill each jar with cold water (not distilled). Let it soak for 5 to 10 minutes.


Drain by placing the lid over the top and leaving a gap between the lip of the jar and your lid. Drain it well. The grains will be quite wet at this point.


For spawning my tubs, I layer my substrate and spawn, then mix it. This way I can minimize how much time I spend turning the substrate before it is evenly mixed.

Some will argue that layering is more ideal, but I disagree. Mixing evenly gives much speedier colonization, which is always a plus. Shroomerite toxetel provides a nice demonstration of this:
Quote:

toxetel said:
Here's a visual aid. It's a very basic simulation of spreading mycelium. Every iteration, each colonized piece has a chance to colonize its neighbors. The white is colonized; the black is uncolonized:






Start by dumping in 3 quarts of pasteurized substrate. Spread it evenly.


Pour the grains into the tub and gently spread them until you have an even layer. I use 3-4 spawn jars for this layer.


I add three more layers- 3 quarts substrate, 4 quarts spawn, 3 quarts substrate.


Now get down there and mix it all together. Make sure to get the corners and then even it out as best you can. It doesn't need to be perfectly level but it doesn't hurt to try :shrug:


Don't use a top layer to cover the grains. It's pointless for cubes and only serves to extend your colonization times.

Cut the liner down to size. You don't want to have any of it hanging over the top of the substrate or covering the bottom holes.


If you haven't already, duct tape your bottom and top holes for colonizing. GE is provided by the lid of the tub.


Put the lid on, label and date with species/variety and let it colonize in a 70-75 degree room with minimal air movement.


Bulk substrates produce a lot of heat while colonizing so you won't want your room temps to get above 80 for any reason.

Depending on your spawn to substrate ratio, colonization should be finished in 7-21 days :thumbup:

Ready to fruit? Here's how I stuff my monos with polyfill for fruiting.

:cheers:







http://www.shroomery.org/forums/showflat.php/Number/18287434#18287434

FH RR both TCs... This is what I was parroting.


I'm not trying to be a dick... I was just sayin.


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EVERYTHING I SAY IS A LIE!!! N ALL MY PICS R STOLEN
WORTH EVERY PENNY!!!!watch it pay for it self in # of high yield flushes!
SO DOPE
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Edited by smegzilla69 (05/20/13 10:33 PM)

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Re: HYDRATING & PASTEURIZING BULK SUBSTRATE PROPERLY [Re: smegzilla69]
    #18295399 - 05/20/13 10:33 PM (10 years, 9 months ago)

:thumbup:


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Re: HYDRATING & PASTEURIZING BULK SUBSTRATE PROPERLY [Re: cronicr]
    #18295520 - 05/20/13 10:55 PM (10 years, 9 months ago)

Those RoggerRabit quotes are funny seeing as how he preaches to do what I'm talking about in his videos and practice on a monthly basis as well, and is where I got my information from.  I still don't see any evidence of me "mostly disagreeing with the "TC's" though.

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Re: HYDRATING & PASTEURIZING BULK SUBSTRATE PROPERLY [Re: hamloaf]
    #18295572 - 05/20/13 11:05 PM (10 years, 9 months ago)

Quote:

hamloaf said:
Those RoggerRabit quotes are funny seeing as how he preaches to do what I'm talking about in his videos and practice on a monthly basis as well, and is where I got my information from.  I still don't see any evidence of me "mostly disagreeing with the "TC's" though.



What I ment was is ur statement contradicted what I have read From TCs

How long ago do u think thos vids were filmed.


--------------------

EVERYTHING I SAY IS A LIE!!! N ALL MY PICS R STOLEN
WORTH EVERY PENNY!!!!watch it pay for it self in # of high yield flushes!
SO DOPE
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Re: HYDRATING & PASTEURIZING BULK SUBSTRATE PROPERLY [Re: agar]
    #18296405 - 05/21/13 04:00 AM (10 years, 9 months ago)

Quote:

agar said:
Quote:

The cooler and pillowcase seems easier...




It does seem that way.
Except, moving / draining is a pita.
Plus, it leaches out lots of optimal nutrients.
(into the water)

More importantly:
With a pillow case of h/poo in a water filled cooler.
You would have to drain/change/add HOT water.
About 3 times during 90 minutes.
For the cooler content to maintain a TRUE CONSTANT 165F temperature.

That's fact.

Poor pasteurization, most often leads to POOR results.
Why risk it?




Agar I want to start by saying you are one of my faves here on the shroomery.  Lots of respect goes out to you.  If I see a thread started by you I know I am bound to learn something invaluable in this great hobby.  Having said that I do not like the statement about coolers not holding their temperature.  Agreed unsuitable for pasteurizing hpoo because of leeching, but they do hold their temps quite nicely therefor it is not fact that you would have to change water three times.  I have been brewing beer for years now using a cylindrical liquid cooler (same as any cooler but with a spigot) for a hot liqueor tank and a mash/lauter tun.  In the mash tun we take a quantity of grain and mix in a quantity of water and hold it at a certain temp for minimum of 90 minutes.  The temp needed (anywhere from 148 to 155 depending on how dry or sweet you want your final product) needs to be maintained for the whole 90 minutes (you mash in, stir, check temp.  wait 45 mins and stir and check temp, then after 90 minutes you check temp and start your sparge).  I have experienced at most a three degree drop in temps during a 90 minutes mash (during a cold winter month if i remember correctly.)  Coolers are great for holding temps and furthermore RR notes that anything above 140 is ok for pasteurization of bulk.  If you got the temp to 165 I would have a hard time believing it would ever go below 160 in 90 minutes unless you had it in a 0 degree celcius environment.  So if one was able to calculate in weight how much hpoo to water one needed for optimal moisture content, you can use online calculators to figure out a "strike temp" for your water (probably around 12 degrees higher than you need it to be to compensate for the cool hpoo) and mix it in well and leave be.  Therefore no leeching of nutrients and proper pasteurizing temps.  That is all theory though.  I still pasteurize in qt jars ontop of the stove anyhow checking for proper moisture content every step of the way like you describe (especially after my poor luck with proper moisture content of coir I have had lately)  Check, then check, then recheck.  Always the way to go!


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Re: HYDRATING & PASTEURIZING BULK SUBSTRATE PROPERLY [Re: agar]
    #24981564 - 02/10/18 11:51 AM (6 years, 1 month ago)

So I'm noticing that when I follow directions, i end up getting different results than the person who wrote up the directions.

I just did my first bulk substrate yesterday. I was in a big time crunch, and the substrate was too wet (I think).

Is there any way to safely dry out the substrate a little to make a better growing environment? The spawn was already added...

I've heard that it's the evaporation that starts the pinning process, so I'm nervous to just crack the monotube to let it dry out that way...

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