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theanarchyway
funknchunkbuds


Registered: 02/28/07
Posts: 70
Last seen: 17 years, 4 months
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need some advice on what to do next...
#6790372 - 04/15/07 12:50 PM (17 years, 9 months ago) |
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Ok I have a colonized pint jar of wbs in the fridge. I have ingredients to do pf jars. Whats a good way to transfer the mycelium. I was thinking of the mycelium inoculation tech. What you guys think. Im going to get a pressure cooker and items for a glove box here in a bit. I could do more wbs but I really want to do pf tek jars too. Any ideas, anything what be helpful thank you.
-------------------- Everything I say on here is Fictional.
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theanarchyway
funknchunkbuds


Registered: 02/28/07
Posts: 70
Last seen: 17 years, 4 months
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Re: need some advice on what to do next... [Re: theanarchyway]
#6794852 - 04/16/07 12:50 PM (17 years, 9 months ago) |
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bump anyone?
-------------------- Everything I say on here is Fictional.
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Pr0_X
CultivationLifer


Registered: 01/18/06
Posts: 617
Loc: Igloo's and polar bears
Last seen: 16 years, 11 months
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Re: need some advice on what to do next... [Re: theanarchyway]
#6795071 - 04/16/07 01:53 PM (17 years, 9 months ago) |
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G2G transfers are very inpractical to do for the average home cultivator. you can not transfer grains to a pfcake anyways since there is no way to get the grains into the cake after sterilization without contaminating the substrate.
You could however use oven bag's and put your pfcake substrate into it and wrap it tight with eletrical tape and doing the transfer in a glovebox but even this does not guarntee anything. I've attempted several G2G in a GL without much success. Your best course of action is to spawn your WBS to straw and or straw/wbs and take spore prints and use those to inoculate the cakes.
Have fun experimenting.
-------------------- It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake
- Support the FSR at www.fsrcanada.com and www.fsre.nl
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theanarchyway
funknchunkbuds


Registered: 02/28/07
Posts: 70
Last seen: 17 years, 4 months
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Re: need some advice on what to do next... [Re: Pr0_X]
#6810592 - 04/19/07 09:42 PM (17 years, 8 months ago) |
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really what I was asking was to attempt something like the mycelium inoculation technique, like in a glovebox take a chunk of the colonized mycelium after dunking and put into h202 water for like 5 minutes then put it into a jar of sterilized water shake around with something in it to break the chunk apart, suck up water into sterilized syringe, use as is. Is this logical? sounds logical to me, but I could be wrong.
-------------------- Everything I say on here is Fictional.
Edited by theanarchyway (04/19/07 09:44 PM)
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theanarchyway
funknchunkbuds


Registered: 02/28/07
Posts: 70
Last seen: 17 years, 4 months
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Re: need some advice on what to do next... [Re: theanarchyway]
#6821216 - 04/22/07 10:42 PM (17 years, 8 months ago) |
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bump
-------------------- Everything I say on here is Fictional.
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agar
old hand



Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: need some advice on what to do next... [Re: theanarchyway]
#6821277 - 04/22/07 11:02 PM (17 years, 8 months ago) |
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