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musher_420
Stranger

Registered: 08/01/05
Posts: 2,691
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Re: Seriously: How CLEAN is your cultivation process...REALLY? [Re: EntheogenicPeace]
#6790010 - 04/15/07 10:25 AM (17 years, 9 months ago) |
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I agree with Agar and Hyphae on the point that the cleaner the better.
But in some cases people will spend a lot of time trying to make things clean and yet they still haven't obtained aseptic conditions. Lets face it, to get even a small room free of all contaminating spores it would require a pretty big investment. HEPA filtering of the air in the room, probably an air lock to enter the room and one of those cool bio-hazard protection suits.
But that's why we use glove boxes and flow hoods. When we really need to be anal about your sterility like working with any opened sterilized media like agar or open grain jars. Also making spore prints or syringes. You can never get too clean for that work.
But when doing work that isn't practically done in a glove box or doesn't really require a flowhood, like spawning to bulk. We can get away with just doing it in a room that is clean but not aseptic.
Don't lose sight of the MOST important aspects before spending extra time on the smaller ones.
I consider the important aspects to be:- Correct water content of jars to ensure quick colonization.
- Using proper filters in lids and sterilizing for long enough.
- Proper pasteurization
- Correct Ph of casing layer <-- Important not just for pinsets but also because green mold thrives in acidic conditions.
- Not over-heating colonizing trays promoting the growth of bacteria.
- Checking jars to be sure their is no unnoticed bacteria or mold.
You can spray all the oust and use all the bleach you want, put on surgical face masks and the whole bit. But if you overlook the stuff above it will all be a waste.
Edit: While I was busting my balls trying to spit out the point I'm making on not overlooking the important aspects RR pretty much covered all my points..and then some..haha..Oh well.
Edited by musher_420 (04/15/07 10:28 AM)
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SmellyMushroom
Top Hat Mushroom


Registered: 02/15/07
Posts: 463
Last seen: 11 years, 11 months
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Re: Seriously: How CLEAN is your cultivation process...REALLY? [Re: Oatman2000]
#6790014 - 04/15/07 10:25 AM (17 years, 9 months ago) |
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I just spray some lysol around in my bathroom, and inoculate my jars without a glovebox, with a dustmask and gloves on.
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hyphae
born to grow



Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 8 months
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Re: Seriously: How CLEAN is your cultivation process...REALLY? [Re: Oatman2000]
#6790044 - 04/15/07 10:45 AM (17 years, 9 months ago) |
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Quote:
Several of my terrariums sit within 18" of a wall that has black mold growing on it, yet it NEVER grows on my cakes.
And it never will. There are a couple of reasons for this for those interested, number one Aspergillus niger is most prevelent in agar and grains not fully colonized cakes which are pretty much resistant to most contams that is one of the beauties of the PFTek it's overall success rate is almost guaranteed if you do it correctly. Trich as well as a few other strains of pathogens are much more an issue when doing other teks and can certainly become a problem. I'm saying this only to enlighten those who maybe wondering why.
-------------------- Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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Sillicybin
Registered: 02/14/05
Posts: 2,134
Loc:
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Re: Seriously: How CLEAN is your cultivation process...REALLY? [Re: RogerRabbit]
#6790077 - 04/15/07 11:02 AM (17 years, 9 months ago) |
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Quote:
RogerRabbit said: When it comes to spawning a bulk substrate, who gives a shit if you pick your nose while working? It doesn't matter. If the grains were properly prepared and colonized in sterile conditions to full colonization, you can spawn to bulk outdoors if you want to.
Yep, this is the main thing. If you have unsuccessful bulk spawning, just use more spawn next time.
If you want to use 1 quart of spawn for 20 quarts of bulk, yes, you'll want to keep it as sterile as possible. If you're doing 1:4 spawn to bulk, you don't really need to go overboard.
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hyphae
born to grow



Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 8 months
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Re: Seriously: How CLEAN is your cultivation process...REALLY? [Re: Sillicybin]
#6790092 - 04/15/07 11:07 AM (17 years, 9 months ago) |
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Quote:
Sillicybin said:
Quote:
RogerRabbit said: When it comes to spawning a bulk substrate, who gives a shit if you pick your nose while working? It doesn't matter. If the grains were properly prepared and colonized in sterile conditions to full colonization, you can spawn to bulk outdoors if you want to.
Yep, this is the main thing. If you have unsuccessful bulk spawning, just use more spawn next time.
If you want to use 1 quart of spawn for 20 quarts of bulk, yes, you'll want to keep it as sterile as possible. If you're doing 1:4 spawn to bulk, you don't really need to go overboard.
Great point bro!
-------------------- Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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coAsTal
Friend



Registered: 04/04/06
Posts: 2,970
Loc: 8a
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Re: Seriously: How CLEAN is your cultivation process...REALLY? [Re: hyphae]
#6790106 - 04/15/07 11:12 AM (17 years, 9 months ago) |
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Every time I read posts like these with the words of the forum masters weighing in, I feel like I did in a math class class when I heard one especially good teacher explain confusing, deeply complex mathematic processes to us in a way that was so..."Well, DUH" that I left completely amazed that a hamster hadn't figured it out by now.
Bravo, good forum people .
-------------------- I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination-- John Keats
Spore Trading List
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Terillius
Renaissance Man


Registered: 07/21/06
Posts: 1,301
Loc:
Last seen: 2 months, 21 days
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Re: Seriously: How CLEAN is your cultivation process...REALLY? [Re: Eraserhead]
#6796360 - 04/16/07 07:21 PM (17 years, 9 months ago) |
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Quote:
Eraserhead said:I'm working on some archaic Fedora Core 3 system right now.
You shouldn't cultivate contams.
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Eraserhead
Lost Soul



Registered: 05/26/06
Posts: 1,363
Loc: Earth
Last seen: 15 years, 2 months
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Re: Seriously: How CLEAN is your cultivation process...REALLY? [Re: Terillius]
#6797599 - 04/16/07 11:12 PM (17 years, 9 months ago) |
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Trust me, that wasn't the goal
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BarakaZulu
Silent Walker


Registered: 04/07/07
Posts: 46
Loc: Darkest Afrika
Last seen: 16 years, 11 months
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Re: Seriously: How CLEAN is your cultivation process...REALLY? [Re: agar]
#6797723 - 04/16/07 11:39 PM (17 years, 9 months ago) |
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Quote:
agar said: The cleaner you can do most of the mush/cult process's involved. The better off you are. Minimal sanitation usually gets you minimal success. (quite often utter failure)
There are a LOT of short cuts. That work sometimes. But, MOST CERTAINLY NOT, all the time.
In a hobby that REQUIRES at least a modicum of patience. It seems many RUSH through things.
In mush/cult the better you do things. Most often get you better results.
Rushing spawn wbs/grain hydration is a mistake. (like ya, just boil the stuff for a 1/2 hour)
Rushing PC sterilization is a mistake. (like ya, 45 minutes @ 12 psi will be okay)
Not letting spawn jars cool to room temps is a mistake. (like ya, they still felt warm, but not real hot when I innoc'ed)
Inoculation should be done as sanitary as possible. (like ya, I just knock-em up on my dirty kitchen counter)
Proper pasteurization is a necessity. (like ya, I just bake it in the oven, or nuke the stuff)
Spawning should be done in most sanitary conditions possible. (like ya, I do it in my dirty kitchen, no mask, gloves, nada)
On & on & on.
Ponder, the bulk of mush/cult posts are about FAILURES. The reason is their own half/ass way of doing things.
Do it right, most often gets you a mushroom crop. Doing things half/ass, gets you a crop of MOLD.
Failure to heed these words is a recipe for a *learning experience* that could have been more fruitful.
- knee deep in fresh crop w00t!
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